Avian Influenza H9N2 and biosecurity - Husam Bakri

Dear brother AHMED ANJUM First, I wish you a happy eid and I would like to apologize for delay to reply to you as I found it on my junk mail. First I prefer to do HI test not elias. HI test more accurate. In Elisa we did not see titer from the vaccine till 28 days. In HI test, usually, we see 3 log2 and some time is less but we have done challenge at 21 days after vaccination at day old we could see good protection even the titer was less than 3log2. In breeder and layer the most important to get 5log2 before production.

Prof. Dr. hussein H Hassanin. First, I wish you and your family a happy eid. Yes, I totally agree with you.

Thank you again for your comments.

I fully agree that broiler vaccination with inactivated vaccines is not the preferred way. However, as you know at this stage we do not have any live H9N2 vaccine and our only option is to use the inactivated vaccine. The usage of the inactivated vaccine in broilers together with the selection of the right clade solved the issue of H9N2 in Israel. Still you need to take into account that early vaccination will be less effective due to MAB effect.
For IBD, NDV and IB there are good and effective live vaccine.

Low pathogenic avian influenza is low pathogenic only in SPF and not in commercial poultry. So-called low pathogenic avian influenza is exacerbated by bacterial and viral agents resulting in mortality even>80%. Further feed and feed ingredients are heavily contaminated with e.Coli/coliforms and other microbial contaminants. Water is sanitized with sanitizing agents/ enviornment is disinfected with disinfectants. But no action for decontamination of feed. Realising the importance of feed decontamination of feed the European union permitted application of formalin 2.5 l per tonne of feed. Formalin 2.5 l+water 2.5 l total 5 l as a fine mist during mixing of feed in the mill. Efficacy of feed decontamination can be screened at feed mill itself as shown here under. Collect 10 g of feed sample morning -a/noon -b/ evening -c
prepare meat extract from day pm cases- 500 g meat add water 1l. Mix it in amixie. Filter through nylon filter. Adjust ph to 7.4 with 1normal sodium hydroxide[sodium hydroxide 4g per 100ml of water]after ph adjustment boil the extract. Filter through fine nylon filter. Sterilize the meat extract in pressure cooker. Weigh 1 g of ttc salt using sterile aluminium foil and sterile spoon and add the same to 100cc of sterile saline mix it well. By means of sterile syringe transfer 3cc of ttc solution to sterile penicillin vials with rubber cork. Store he same in refrigerater. To strile meat extract 1/add 3cc of saly solution mix it well. Distribute the solution in 100 cc penicillin vials as 50cc per vial. To ttc vials add ten g of he collected feed sample mix it well and incubate at incubater or at room temperature. Examine the sample at interval of 6 hours for colour change-pink colour. Colour change12 hours load -3+/>18 hours load is 2+/cplour change >24 hours load is 1+/>24 hours no colour change feed sample is satisfactory-venugopalan -13-8-18

Khalid Ibrahim first I wish you a happy eid. Honestly, mortality always depends on what other diseases playing a role with H9 at the same time. From my experience, the major singes in broiler breeder and layer is drop in production and very low mortality.

Dibesh Thapa salam,
From my experience vaccination in breeder and layer is a must. In broiler is also important but depends on the infection time. If it is before 3 weeks, vaccines will not help at all, if it after yes vaccine will help definitely yes. That why lab tests are important for 2 things:
1- to know what is going on with your farm and if other diseases playing a role to control them.
2- you well when the problem stats.

All the best.