Artificial Insemination: Troubleshooting to Improve Fertility

Published on: 06/13/2018
Author/s : Murray R. Bakst / Consultant in poultry fertility and early embryonic mortality.

Artificial insemination (AI), the foundation of modern-day turkey breeding, was first developed in 1937 at the Bureau of Animal Industry (now the Agricultural Research Service, US Department of Agriculture) by W. Burrows and J. Quinn. The procedure consists of two steps: first, the semen is collected from the tom and second, the semen is inseminated into the hen. What may transpire between collect...

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MUKHTAR AHMAD MUKHTAR AHMAD
Doctor of Veterinary Medicine
June 13, 2018

How long turkey and chicken semen can be stored ...and how many times can be diluted?

Reply
June 15, 2018
MUKHTAR AHMAD
Thank you for the question. Duration of semen storage and subsequent fertility is dependent on the experience of the crew handling the semen and inseminating the hens. With the proper semen diluent and under the right storage conditions, diluted chicken or turkey semen can be stored for 4 hr at around 10C without a negative impact on hen fertility. There are numerous commercially prepared semen diluents to choose from and I would suggest that you follow the accompanying directions with regard to the semen dilution rate (I usually use 1 part raw semen to 1 part diluent), storage temperature, and duration of storage. Remember that when storing turkey semen for longer than 30 min the semen must be agitated (in a shaker at 80rpms) as turkey sperm need to be oxygenated to survive. For more detailed information go to Google Scholar and search poultry semen storage.
Reply
June 15, 2018
MUKHTAR AHMAD: Thank you for the question. Duration of semen storage and subsequent fertility is dependent on the experience of the crew handling the semen and inseminating the hens. With the proper semen diluent and under the right storage conditions, diluted chicken or turkey semen can be stored for 4 hr at around 10C without a negative impact on hen fertility. There are numerous commercially prepared semen diluents to choose from and I would suggest that you follow the accompanying directions with regard to the semen dilution rate (I usually use 1 part raw semen to 1 part diluent), storage temperature, and duration of storage. Remember that when storing turkey semen for longer than 30 min the semen must be agitated (in a shaker at 80rpms) as turkey sperm need to be oxygenated to survive. For more detailed information go to Google Scholar and search poultry semen storage.
Reply
MUKHTAR AHMAD MUKHTAR AHMAD
Doctor of Veterinary Medicine
June 15, 2018
Murray R. Bakst Thank you for your valuable time and information. What are the fertility and hatchability results if we will follow the procedure in chicken.
Reply
MUKHTAR AHMAD MUKHTAR AHMAD
Doctor of Veterinary Medicine
June 15, 2018
Is it economical fr chicken
Reply
June 18, 2018
MUKHTAR AHMAD
If you would like a 115 page manual covering important aspects of poultry artificial insemination and fertility evaluation techniques please email me so I can send a pdf to you..

Actually it is free to anyone who requests it.
Murray Bakst
Reply
MUKHTAR AHMAD MUKHTAR AHMAD
Doctor of Veterinary Medicine
June 18, 2018
Murray R. Bakst
I have gone through some of your research articles regarding AI in poultry. You have done very interesting work on it.
Thank you for sharing knowledge regarding it.
You may reach me by
ahmadmukhtar854@gmail.com
Reply
Sarzamin Khan Sarzamin Khan
PhD, post doc
June 19, 2018
Murray R. Bakst

Thanks sir for this generous offer. Would you please send me a copy on dr.zamin@aup.edu.pk.
Reply
June 22, 2018
MUKHTAR AHMAD
sent manual as requested...good luck
Murray
Reply
June 22, 2018
Sarzamin
I get an error message using dr.zamin@aup.edu.pk. to forward manual.
murray
Reply
MUKHTAR AHMAD MUKHTAR AHMAD
Doctor of Veterinary Medicine
June 22, 2018
Murray R. Bakst
Thank you sir for sharing such a good manual with all of us.
Mukhtar Ahmad
Reply
Silvateam S.p.a. Silvateam S.p.a.
Piemonte, Italy
June 22, 2018
Murray R. Bakst Hi Murray, glad to see you still on the pitch promoting the other "AI". Would you please send me a copy of your PDF containing artificial insemination and fertility evaluation techniques? It occurs to me that an economic evaluation of AI in commercial broiler production may be in order as the production of fertile eggs and baby chicks is a often a bottleneck in the field. All the best, Ernie
Reply
June 23, 2018
Murray R. Bakst its good information sir please send .pdf if possible .
My mail id is jodhsinghtoor@gmail.com
Reply
Harun Ur Rashid Harun Ur Rashid
Doctor of Veterinary Medicine,Post Graduate Diploma in International Poultry Husbandry from Barneveld College The Netherlands.Post Graduate Diploma in Animal Feed Training Program fom Barneveld Colleg
June 23, 2018
Murray R. Bakst
Greetings!
I would like to get one copy.
harun@index-agro.com
Thank you.
Reply
June 24, 2018
E. Ernest M. Pierson hey Ernie, hope all Is good for you and yours. An economic evaluation of artificial insemination has not been done, to my know knowledge, since the 90s. Please forward your email to me and I will forward the workbook to you. Murray
Reply
Sarzamin Khan Sarzamin Khan
PhD, post doc
June 24, 2018

Murray R. Bakst
Thanks. One of my PhD student has worked on economic evaluation and hope I will share that soon after get published.I am working on the same project for looking the feasibility of shifting broiler breeder in cages to reduce health problems, feed cost and augment farmer's income through bio gas production and organic manure for orchards. AI will be the only option for fertility in that case. We have large number of no or low educated labor who could be trained and employed as AI technicians. Your inputs through my E mail will help me please
My email ID is: dr.zaminaup@gmail.com

Reply
June 28, 2018
Murray R. Bakst

Thank you for offer. kindly send me a pdf copy of Poultry artficial insemination and fertility evaluation manual. I will be grateful
Reply
Ugur Akbas Ugur Akbas
Veterinary Doctor
June 29, 2018
MUKHTAR AHMAD

Thanks a lot for sharing this valuable information.

I'd like to have the copy.

akbasugur@gmail.com
Reply
June 30, 2018
Peter Njuguna Kamau ...Please send your email address.
Reply
July 3, 2018
Please send me a copy @ tuyosi1706@yahoo.co.uk
Reply
Ashra Ali Qureshi Ashra Ali Qureshi
Doctor of Veterinary Medicine
June 15, 2018

Ideally, semen storage of cockrals will reduce the ability to fertilize germinal disc even if dilution or extender is used to store semen in a refrigerator. For best results use fresh semen mixed with 30% extender immediately and inject within 7-10 minutes of collection in summer and 10 to 15 minutes of collection in winter.

Reply
June 16, 2018
Does anyone can advice AI on swine?. Sorry
Reply
June 22, 2018
David Hoo
Use the internet ... all that is printed is there. Try google or google scholar...Good luck. Murray
Reply
Carlos Avilan Carlos Avilan
Community Manager
June 22, 2018

David Hoo You can read this technical article on Engormix: https://en.engormix.com/pig-industry/articles/artificial-insemination-pigs-research-t40422.htm.

If you need more information on the subject, click here: https://en.engormix.com/pig-industry/articles/swine-genetics-reproduction-103-324/.

Kind regards.

Reply
mst.dilruba begum mst.dilruba begum
Bsc and Msc
June 19, 2018
Dear sir, summer greetings from bangladesh and many more thanks for your consistent efforts. point to be noted that i have done MS degree for frozen semen production in black bengal goat with the assistance finance by USDA project. pls give me your valuable pdf file for research. SIR you will reach me through fk_ruba@yahoo.com

With regards
fazlul karim
poultry development officer
govt. poultry farm, rangpur, bangladesh.
Reply
June 22, 2018
mst.dilruba begum
Forwarded to your email as requested...Murray
Reply
June 26, 2018
I have been teaching poultry production for Veterinary students. Your Information may be helpful. Send at- zaman_65@hotmail.com
Reply
June 27, 2018
sent copy to
zaman_65@hotmail.com
Reply
Narayan Reddy Dasari Narayan Reddy Dasari
Veterinary Doctor
June 28, 2018
Murray R Bakst sir can you please send copy of A I in poultry by mail My mail ID is. narayandasari@hotmail.com
Reply
Ashra Ali Qureshi Ashra Ali Qureshi
Doctor of Veterinary Medicine
June 28, 2018
Murray R Bakst

Please send me a copy as well. My email is aaqureshi6666@hotmail.com. Thanks
Reply
Greta Van de Sompel Greta Van de Sompel
Doctor of Veterinary Medicine
June 28, 2018
Dear Sir,Thank you very much for your offer.
Can you please send a copy of AI in poultry to my email adress:
johan.van.der.cruyssen@telenet.be
Kind regards
Reply
June 29, 2018
Hello Murray great article
Reply
June 30, 2018

William, thanks. Murray.

Reply
David S. O. K. Quartey David S. O. K. Quartey
B.Sc (Agric), M.Phil (Animal Nutrition)
July 17, 2018
Ineresting. Could you please send me a copy?
davidquartey61@gmail.com
Thank you
Reply
Hanaa Amin Hanaa Amin
Research
September 29, 2019
Dear Dr Bakst, thank you for your article. Could you please send me a copy to my email address:
ahanaa43@yahoo.com
Thank you
Reply
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