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Evonik Animal Nutrition

Evaluation of methionine sources on performance and carcass traits of broilers at different dietary sulfur amino acid levels under northern European and middle Eastern conditions

Published: August 18, 2023
By: Zeyang Li 1, Juliano Cesar De Paula Dorigam 1, Ali Afsar 2, Andreas Lemme 1, Gabriel da Silva Viana 3, Ehsan Musharbash 4. 1, Evonik Operations GmbH, Hanau-Wolfgang, Germany 2, Evonik Iran AG, Teheran, Iran 3, Natural Resources Institute Finland (Luke), Jokioinen, Finland 4, Alestesharia research farm, Amman, Jordan
DL-Methionine (DL-Met, 99%) and liquid DL-2-hydroxy-4-methylthio butanoic acid (methionine hydroxy analogue-free acid, MHA-FA, 88%) are often used in commercial poultry feeds to meet the requirements for Methionine+Cysteine (M+C). However, differences in chemical properties and absorption decrease the relative bioavailability value (RBV) of MHAFA, which corresponds to 65% of DL-Met for performance .
This study aimed to confirm that MHA-FA can be replaced with DL-Met at a weight-to-weight ratio of 100 to 65 under different rearing conditions. In total, 720 (Trial 1: Finland, 5 treatments with 9 replicates and 16 males per replicate, wheat soybean based feeds) or 2500 (Trial 2: Jordan, 5 treatments with 10 replicates and 25 males and 25 females per replicate, corn-soybean based feeds) day-old Ross 308 broilers chicks (~41 g) were used.
In both trials, broilers received a basal diet (BD) formulated to meet the nutritional requirements except for additional Met sources (60-66% of M+C requirements), or BD supplemented with MHA-FA or DL-Met (added at 65% of MHA-FA, w/w), to reach 75% (75MHA-FA, 75DL-Met) or 100% (100MHA-FA, 100DL-Met) of M+C requirements. Broilers were fed ad libitum a 3-phase program during day (d) 0-35 (Trial 1) or d 0-32 (Trial 2) under standard housing conditions. Bodyweight (BW), feed intake (FI), daily feed intake (DFI), bodyweight gain (BWG), daily weight gain (DWG) and feed conversion ratio (FCR) were measured in Trials 1 and 2. Carcass (CY) and breast (BY) yields were measured on d 32 in Trial 2. Data was analyzed using one-way ANOVA with GLM procedure of SAS (ver. 9.4).
Significances were considered if P< 0.05 (Tukey test). In Trials 1 and 2, MHA-FA and DL-Met improved (P< 0.01) FI, DFI, BW, BWG, DWG and FCR compared to BD, without any differences between MHA-FA and DL-Met groups within each M+C level. In trial 1, the 100% M+C groups had higher BW (+4.68%, P< 0.01), DWG (+4.76%, P< 0.01) and lower FCR (-4.04%, P< 0.01) than 75% M+C groups, while DFI did not differ between 75% and 100% M+C levels during d 0-35.
In Trial 2, the 100% M+C groups had higher (P< 0.01) BW (+4.27%) and BWG (+4.34%), and similar FI (P> 0.1) compared to 75% M+C groups, whilst FCR was only lower (-3.54%, P< 0.01) in 100MHA-FA vs. 75MHA-FA groups. There was no difference in CY and BY between MHA-FA and DL-Met groups within each M+C level. Breast yield was higher (P< 0.01) in 100% and 75% M+C vs. BD groups (+16.62%, +8.40%) and in 100% M+C vs. 75% M+C groups (+7.58%), whilst CY was only higher (+3.44%, P< 0.01) in 100% M+C vs. BD groups. In conclusion, the results confirm that 100 units of MHA-FA can be substituted by 65 units of DL-Met for broilers at the same M+C level, regardless of the regional differences.
Keywords: Nutrition, poultry, amino acids, performance, methionine
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Authors:
Juliano Dorigam
Evonik Animal Nutrition
Evonik Animal Nutrition
Ali Afsar
Evonik Animal Nutrition
Evonik Animal Nutrition
Dr. Andreas Lemme
Evonik Animal Nutrition
Evonik Animal Nutrition
Dr. Zeyang Li
Evonik Animal Nutrition
Evonik Animal Nutrition
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Dr Valeriy Kryukov
3 de enero de 2024
Dear sirs!
In general, researchers correctly establish in specific experiments the comparative effect of D,L-methionine and its hydroxy analogue, However, as I wrote earlier, it must be taken into account that D,L- unlike L-methionine stimulates the xenobiotic metabolism system, that is, it increases the body's protection from harmful substances entering the feed as "pollutants" and even an excess of vitamins.the effect of the hydroxyanalog of methionine in this direction has not been studied.
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Mike Stahl
2 de enero de 2024

Would anybody be able to show any differences in DL vs MHA in layers?

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Alireza rostamkhani
20 de agosto de 2023
there is nice report. We replaced in diet 65 part of DL-MET with 100 part of MHA in trial on 6000 broilers in iran. Results indicated that body weight and FCR were the same in both groups confirming 65% RBV of MHA.
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