Enzymes and their impact on amino acid digestibility

Hello Dr. Cowieson, Hope you are doing well. I wondered whether you have any thoughts/insights on the effects of exogenous proteases (specific or generally) on proteinaceous, immunogenic, components of the microbiota or microbiome and whether exogenous protease use been associated with (e.g. downregulation of) immune markers in different gastrointestinal tissues? The performance response to doses of exogenous protease(s) generally appears to track its dietary substrate availability and/or there could be scope for a microbiome-mediated element? Many thanks

Thank you for the thoughtful and useful considerations, Dr. Cowieson. All the best.

Aaron Cowieson Thanks for your explanation. How if we have a combination of trypsin-like and chrmotrypsin-like proteases?

Hello Dr. Cowieson, Do you mind commenting on the following assertion in a different Engormix forum? "Trypsin and chymotrypsin inhibitors will also inhibit the activity of trypsin-like proteases, such as serine proteases, see Morita (1996) Journal of Biochemistry, 119(4), 711–718. In that respect, you will probably fare better with aspartic proteases, which are resistant to trypsin (Kunitz) and trypsin and chymotrypsin (Bowman-Birk) inhibitors" Thank you.

Thanks, Aaron Cowieson.

Thanks, Aaron Coweison.

Hello, Aaron Cowieson hope you will be fine. Have you any idea about the effect of exogenous protease enzyme on the endogenous secretion of protease enzyme? Exogenous protease enzyme increase or decrease the secretion of endogenous protease enzymes?