Egg production and quality responses to increasing isoleucine supplementation in Shaver white hens fed a low crude protein corn-soybean meal diet fortified with synthetic amino acids between 20 and 46 weeks of age

Ilona Parenteau, As in the market we have synthetic lysine to valine, the study with isoleucine to define a better relationship with lysine is of fundamental importance, since this amino acid is the possible limiting factor after valine, so it is the isoleucine x lysine relationship that defines maximum level of protein reduction that can be used since there is no commercially available isoleucine. And of course, to use valine, this information is a reference.
As this amino acid is the limiting factor after valine. As a suggestion, I would like to comment on the results we obtained in studies conducted with laying hens, the ideal ratio between sulfur amino acids (MET + CIST) with lysine is 100: 100. Finally, I congratulate the authors for using a sub-optimal level of lysine, which is the most correct practice of determining the amino acid relationship between any amino acid and lysine.