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Efficacy evaluation of topical herbal spray in subclinical Mastitis

Published: June 18, 2018
By: Dr. Amit Kumar Pandey Ph.D. (VPT), Dr. Praful Kumar. Ayurvet.
In today’s world safe and wholesome milk production is a challenge for the farmers with increasing incidences of different types of diseases and emergence of new and resistant pathogens due to indiscriminate and unjustified uses of antibiotics/ antimicrobials. When we are talking about the prevalent diseases Mastitis one of the major disease which cause trouble not only to animals and farmers but significant loss to the economy of the farm, society and ultimately to the country.
Bovine mastitis defined as ‘parenchymal inflammation of the mammary gland’ is characterized by a range of physical and chemical changes of the milk and pathological changes in the udder glandular tissues. According to the clinical symptoms mastitis may be classified as clinical mastitis or sub- clinical mastitis. Subclinical mastitis usually leads the clinical form as it is of longer period, difficult to diagnose, adversely affects milk production and quality and comprises a reservoir of pathogens that lead to disease of other animals within the herd. Mastitis is the most cost intensive production disease in dairy industry, causing a considerable financial burden. According to a recent report an annual economic losses sustained by dairy industry in India on account of udder infections have been projected about Rs. 6053.21 crores. Out of this, loss of Rs. 4365.32 crores (70%-80% loss) was credited to sub clinical version of udder infections. Subclinical mastitis is important due to the fact that it is 15 to 40 times more prevalent than the clinical form.
Control of bovine mastitis is challenge because of multiple etiological agents. Mostly antibiotics are used for the treatment and control of mastitis, but intra-mammary infusion of antibiotics for mastitis therapy was cited as a major reason for milk contamination and frequent use of antibiotic therapy leads to antibiotic resistance. Increasing emergence of antibiotic resistant pathogens is further suspected to complicate the effectiveness of the mastitis treatment. WHO has also emphasized on the use of medicinal plant as an alternative to antibiotic. Several herbal extracts have shown in vitro antibacterial activity versus major mastitis pathogens. Some of these are Cedrus deodara, Curcuma longa and Eucalyptus globules which is having anti-inflammatory effect also. Detecting the mastitis in early stage and keeping the animal udder in the outmost healthy condition is the only way to prevent the physical and economic losses due to mastitis. There should be a solution which is giving all round protection to udder not only from the pathogens but also relief the pathogenic effects to the udder. All the above mention recommendation was fulfilled by a Mastilep which is an herbal spray. We keep continuing our research studies through clinical and field trial to ascertain the efficacy of products at different situations. On this context we are having a clinical trial report in which the efficacy of the herbal spray for the treatment of mastitis was compared with another herbal product which is very much known in the market.
Plan of Trial:
A total of 30 cows were screened as per the guidelines of International Dairy Federation (IDF) having 10 healthy and 20 cows were exhibiting the signs of subclinical mastitis, divided in to 3 groups. Control; having 10 healthy cows, Second group having 10 cows suffering from SCM and treated with ‘Brand A’ (gel), applied gently by massaging the udder after each milking, BID for 5 days. Third group is also having 10 cows suffering from SCM and treated with Mastilep spray BID for 5 days. Therapeutic efficacy was determined on the basis of Modified California Mastitis Test (MCMT) readings, improvement in the somatic cell count and milk yield.
Result:
Different
Modified California Mastitis Test (MCMT)
Table 1: MCMT Test.
Efficacy evaluation of topical herbal spray in subclinical Mastitis - Image 1
MCMT at day 0, 5th, 14th and 21st. In the ‘Brand A’ treated group after the treatment on 5th day, 14th day and also on 21st day, 60% animals were found to be positive. In the Matilep treated group after the treatment on 5th day 60% animals were positive and only 20% remained positive at 14th day whereas on day 21st it was only 10% remained positive. Data shows the high antimicrobial potential and anti-inflammatory properties of Mastilep against the subclinical mastitis.
Table 2: Average Somatic cell count (SCC X105).
Efficacy evaluation of topical herbal spray in subclinical Mastitis - Image 2
In the control group the average Somatic cell count (x105) ranges between1.15 to 1.67. Average SCC (x105) of ‘Brand A’ treated group there is 16.94 % decrease in the SCC from day 0 to 21st whereas in Mastilep treated group there is 78.52% decrease in the SCC from day 0 to 21st. Decrease in the average SCC is due to the antimicrobial and anti-inflammatory properties of the herbal ingredients of Cedrus deodara, Curcuma longa and Eucalyptus globules plants which are the component ingredients of the herbal spray Mastilep.
Milk Yield
Table 3: Milk yield during the study period between the treatments.
Efficacy evaluation of topical herbal spray in subclinical Mastitis - Image 3
There was a significant increase in the average milk production of Mastilep treated group, from 9.040 L /day on day 0 to10.63 L /day on day 21st. Whereas there was no significant increase in the average milk production of ‘Brand A’ treated group and control group. Significant increase in the average milk production is may be due to anti-inflammatory and antimicrobial property of the ingredients of herbal spray Mastilep which leads to quick recovery of the mammary glands from the infection and inflammation consequent upon which mammary gland becomes healthy and more milk produced by the gland.
CONCLUSION:
Result shows that the efficacy of the herbal spray ‘Mastilep’ was better than that of ‘Brand A’ treated group. Herbal spray Mastilep has proven excellent in the form of improved SCC and increased milk yield. Therefore application of the herbal spray.
Mastilep may be recommended to cure subclinical mastitis.
This article is based on report ‘Efficacy evaluation of topical herbal spray in subclinical Mastitis’. Trial study was done at TANUVAS, Chennai, India published in wjpmr, 2017
For further information please Contac below: Praful Kumar, or AMit Kumar Pandey.
Related topics:
Authors:
Dr. Amit Kumar Pandey
Ayurvet Ltd.
Praful Kumar
Influencers who recommended :
Dr. Amit Kumar Pandey
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Jasmer Singh
3 de marzo de 2022
Well continue this vague conversation and the best would be expected after a long wait...?
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Sanjay Patil
dsm-firmenich
4 de noviembre de 2019
Supplementation of Vit A 1lac IU, D3 20000 IU with Vit E 4 gm and organic Zinc @4gm /day would be beneficial in reduction of subclinical mastitis by improved immune status. Also regular inclusion of these will be effective to reduce SCC.
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Dr. M.N.balakrishnan Nair
17 de octubre de 2018

The spray which we are using is now under field trial will be ready for manufacturing and marketing soon. The formulation which we are using is good for all type s of mastitis with 91.7 per cent efficacy. The study on the microbiome of the udder when treated with this formulation is underway.

I was trying to get the details of the Ghana conference but was not able to get it.
I shall be thankful do anybody who can provide the details to me.

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Viswanatha Reddy
21 de septiembre de 2018

Dr.Balakrishna Nair,

Probably after feeding lemon with an interval of an hour feeding 2 to 3 Garlic bulbs of 2 inch size (diameter) can further improve the results.
To prevent mastitis, few farmers use the practice of feeding garlic bulbs after triturating and feeding with grass or concentrates. Looking in to this Veterinarians do advice to feed Garlic bulbs in mastitis cases along with allopathic treatment.

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Dr. M.N.balakrishnan Nair
21 de septiembre de 2018

We have been using this with constant successful result if you follow the SOP.
We are working on a product, which will reduce the number of applications. Until that time one has to follow the protocol if using this formulation.

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Dr. M.N.balakrishnan Nair
20 de septiembre de 2018

300 grs of aloe Vera, 50 grs of turmeric 10 great calcium hydroxide, grind them well, divide it into 10 equal parts, take one part dilute it with 150ml of water.
Wash the udder, remove the milk completely apply the formulation all over the udder with a massage for 2 minutes
After one hour wash the udder take one part of the prepared medicine and dilute it with 150 my of water and apply it on the under repeat it for 10 times in a day.
This treatment has to continue for 5 days. Besides the application give two lemon in the morning, afternoon and evening by cutting it into 4 pieces touch it on salt and feed the animals within 3 days mastitis will be controlled.

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Dr. M.N.balakrishnan Nair
20 de septiembre de 2018

Dear Dr. Sharen.
We have trained some vets and farmers on Herbal formulation in Chandigarh.
I can send the list if you want.
NDDB is also now want to have the second phase of training where some of the people from your place participate.
Nair

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Dr. M.N.balakrishnan Nair
20 de septiembre de 2018

NDDB is running a Mastitis control popularization programme in which they tried trisodium citrate for controlling sub clinical mastitis and was able to succeed to certain level but they were not able to control the acute or chronic mastitis.
We have a joint programme with them now very successfully managing mastitis and other 14 conditions without intervention of any other chemical drugs. We have formulation for external application for mastitis (any type) and we give 2 lemons 3 times a day for 5 days with 92 % success. FMD 99%, diarrhea 100%, Udder pox 100%, Enteritis 100%, repeat breeding 60%
teat obstruction 99% and so on.
We have designed these formulations in such a way that the farmers can prepare these at home and treat the animals. we can use these formulations for preventing these conditions.
The formulation for mastitis is being made into a spray. Our intervention impact analysis shows 85% reduction of antibiotic residue in the milk in 10 units of milk collection center
We use then as Ayurveda products because we validate them using Ayurveda System. However, we also do study using western science protocol where ever it is possible.

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Viswanatha Reddy
17 de septiembre de 2018

From the literature referred by the veterinarians of Bangalore Milk Union they started testing the milk poured by each member of the Milk Co-op society with California Mastitis test in which they use equal quantity of milk and reagent. Whichever milk was found positive the animal or animals in his house are tested individually from each of the lactating teat. If the lactometer reading or fat test if they show below the mark then also individual animal is tested with California Mastitis Test. Animal found positive for the test is treated with encapsulated Tri Sodium Citrate at the rate of 30 mg per kg body weight once a day for 10 days. Usually, they give 20 grams of Ban Mast 50. On 11 or 12th day the CMT is repeated if positive another 10 days same dosage is repeated. Usually, 80 per cent of cases recover. Those which fail to recover are treated with antibiotics. Earlier the Clinical mastitis of emergency cases were 23 per cent, now it is between 4 to 6 per cent.

Few farmers do feed once or twice a week 500 grams of Germinated horse gram after grinding and boiling with salt or Jaggery with the idea of supplementing Vitamin E and selenium (legumes will have more selenium) The purpose of boiling the material is to destroy the urease enzyme which is proteolytic. Without boiling when fed it has shown a drop in the milk yield. Feeding 3 garlic bulbs of 2 inch size (triturated) is also practiced by few farmers once a week.

Dr.V.N.Viswantha Reddy
Former Professor Animal Reproduction
Veterinary College, Bangalore

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Dr. M.N.balakrishnan Nair
16 de septiembre de 2018

We are possible to explain with a substantial evidence how the herbal preparations work but as i mentioned not completely on western parameters. But I sincerely believe that evidence must be generated whether by Ayurveda or what ever possible way the western parameters fit into the regime
I respect all knowledge system and therefore. I would prefer not to comment on what I do not know rather than telling this is not science
I am now working on the microbiome of the udder before and after treatment of the herbal formulation which can be an indication of cure
We have done a clinical study with parameters like pH, Electrical conductivity and SCC which shows the formulation works well for controlling mastitis.

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