Colibacilosis, a scary and economically devastating disease
Published: July 14, 2021
By: PlusVet Animal Health
With this post, a general introduction to the disease, we start a series of articles about avian colibacillosis.
Colibacilosis is one of the most common infectious bacterial diseases in the poultry industry and one of the main causes of economic loses. Such losses are caused by increased mortality and morbidity, lack of uniformity, decreased production, treatment costs and increased condemnation at the slaughterhouse. It affects broilers, layers and breeders of all ages, although younger animals are generally more susceptible to the disease.
Colibacillosis is defined as an infection or disease caused by colon bacilli, especially E. coli. E. coli is a normal inhabitant of the digestive tract and it is widely disseminated in the bird’s skin and feathers, feces, litter, dust and in general throughout the environment of the poultry house. Moreover, feedstuffs, drinking water, rodents, mites and insects all can be a source of E. coli. However, only 10 to 15% of the E. coli strains are pathogenic and are called Avian Pathogenic Escherichia Coli (APEC) strains. The main serotypes involved in colibacilosis are O78, O2 and O1.
Colibacillosis starts whenever APEC enters the body and begins a localized or general infection. Healthy animals do not develop colibacilosis: the disease happens when the birds are debilitated due to a previous infection, poor nutrition, toxins, or environmental stress.
Routes of entry of Avian Pathogenic Escherichia Coli
APEC may enter the body through 6 main routes:
1. Respiratory tract
The main transmission route of APEC in poultry is through the inhalation of contaminated air or dust.
APEC can start a respiratory infection by itself or, most commonly, lead to a secondary bacterial infection, especially whenever there is an existing infection by virus or bacteria (Newcastle disease virus, infectious bronchitis virus, M. gallisepticum, P. multocida, infectious laryngotracheitis), irritation or secondary reactions to vaccines.
Moreover, as we explained in this article, in order to allow a faster oxygen exchange, the respiratory membranes are thinner in avian lungs than in mammals. A thinner interface makes gas exchange more efficient, but at the same time it is easier for airborne pathogens like APEC to cross the barrier and start a systemic or localized infection inside the body
2. Digestive system
An imbalance of gut flora caused by bacterial infections, coccidia, mycotoxins, poor water or feed quality, feed changes, stress or the administration of antibiotics may lead to the overgrowth of APEC.
Whenever there is a disruption of the gut barrier, APEC is able to cross the digestive epithelium, arrive to the circulatory system and start a localized or systemic infection.
3. Skin
The skin is the body’s first line of defense. When the skin is broken or disrupted, such as in case of footpad dermatitis, wounds or scratches, microorganisms colonizing the skin or introduced to the wound can cause an infection.
Pathogenic bacteria can also spread through the tissues and cause cellulitis (bacterial infection of the dermal or subcutaneous layers of skin), osteomyelitis (bacterial infection of the bone) or septicemia (bacterial presence in the blood that can lead to a whole-body inflammatory state). The risk is higher when the bird’s immune system is compromised.
Cannibalism and vent peaking have been proved to be linked to the onset of colibacilosis.
4. Reproductive system
APEC ascending from the cloaca, coming from the environment or from the digestive system, travels up through the oviduct to the ovary and causes infections in the reproductive system or in the body cavity.
Whenever the oviduct is infected, yolks may be laid outside the oviduct and lead to egg yolk peritonitis.
5. Navel infection
Navel infection, also called omphalitis, affects day-one-chicks and is caused by a number of bacterial species, including Staphylococci, Proteus, Pseudomonas and E. coli.
The navel is inflamed and doesn’t close, opening the door to infections in the abdominal cavity and to septicemia.
6. Vertical transmission
As we described in this article, APEC can be transmitted from breeders to chicks in two different ways:
>Vertical transmission
During the egg formation, the albumen or the egg membranes (rarely the yolk itself) become contaminated as a result of an existing APEC infection in the ovaries or in the oviduct. The eggs can also be contaminated during the passage through the cloaca.
>Horizontal transmission
APEC arrives inside the egg from the outside environment by penetrating the eggshell. Although huge microbial contamination of the eggshell is not common at the time of oviposition, avian faecal material and other environmental sources in the laying house, during transport, storage or incubation can rapidly introduce bacteria into eggs.
Localized and systemic infections caused by Avian Pathogenic Escherichia Coli
By definition, the term colibacilosis refers to any infection caused by APEC. The details of each type of infection will be described in coming articles, but in general we divide them as follows:
Localized infections
>Omphalitis (navel infection)
>Cellulitis (infection of the dermis)
>Swollen head syndrome
>Salpingitis
>Peritonitis
>Orchitis
>Airsacculitis and pneumonia
>Cellulitis (infection of the dermis)
>Swollen head syndrome
>Salpingitis
>Peritonitis
>Orchitis
>Airsacculitis and pneumonia
Systemic infections
Colisepticaemia is a generalized infection of the bird’s body by APEC. It affects chicken of all ages, but especially young chicks and hens at the onset of egg production. It causes depression, fever, dirty vents and often high mortality, with sudden death. The infection of the heart leads to cardiac failure.
Polyserositis (perihepatitis, pericarditis) and peritonitis can be observed in the necropsy.
The birds that survive may show sequelae such as osteomyelitis, synovitis, pericarditis or salpingitis.
Risk factors
As stated above, colibacilosis is a disease that happens in debilitated animals, as a result of a decline of immunity or when there is a high pathogen load in the environment. The main predisposing factors are the following:
>Respiratory challenges, such as viral diseases and mycoplasmosis
>Excessive concentration of dust and ammonia in the environment of the house, which harms the cilia of the upper respiratory tract and make the respiratory system more susceptible to APEC
>Secondary reactions to vaccines
>Deficient hygiene of the poultry house
>Stress, including heat or cold stress, overcrowding, noises and feed changes.
>Digestive infections, including parasitic diseases, coccidiosis and necrotic enteritis, as well as digestive imbalances
>Mycotoxicosis
>Poor microbiological quality of feedstuffs or drinking water
>Mites, flies, insects, and rodents
>Cannibalism, vent peaking, footpad dermatitis and other lesions on the skin
>Poor day-one-chick quality
>The strain and virulence of the APEC and the microbial load
Treatment
Colibacilosis is mainly treated with antibiotics.
While 10 years ago antibiotics used to be remarkably effective, due to antibiotic abuse nowadays many strains of APEC are resistant to multiple drugs.
APEC strains are especially resistant to tetracyclines, sulphonamides, streptomycin, trimethoprim, nitrofurans, nalidixic acid and ampicillin.
Amoxycillin, neomycin (digestive action only), gentamycin and fluoroquinolones show better effectiveness.
Prevention
The effective control and prevention of colibacilosis relays on identifying and eliminating the risk factors of the disease. By improving biosecurity, sanitation, air quality, nutrition, immunity and quality of the day-one chick, it is possible to reduce the level of exposure to APEC.
1. Biosecurity and sanitation
>Cleaning and disinfection programs to reduce the APEC microbial load in the farm.
>Improvement of the microbiological quality of the litter
>Protection against pathogens that promote infections with APEC, such as respiratory virus, Mycoplasma sp., coccidia.
>Design of a complete vaccination program
>Frequent collection of dead animals
>Monitoring of the birds for feather cover, footpad dermatitis, cannibalism, wounds.
2. Air quality
>Reduction of dust and ammonia
>Proper ventilation of the house
>Optimal environmental temperature and moisture
3. Nutrition
>Improvement of the feed digestibility
>Reduction of the bacterial load of the feed
>Control of the microbiological quality of drinking water
>Use of a water sanitizer, such as DigestoCid© together with chlorination
>Use of feed additives for gut health, to reduce the amount of APEC in the intestines and to reinforce the gut barrier
>Minimize feed changes and ingredient changes in debilitated or stressed flocks
>Control of the levels of mycotoxins
4. Immunity
>Prevention of diseases that lower immunity, such as IB, mycoplasmosis, mycotoxicosis
>Optimization of the vaccination program
>Control of the secondary reactions to vaccines
>Promotion of the healthy gut flora
>Use of feed additives with immune boosting effects
>Reduction of stress
5. Quality of the day-one-chicks
>Reduction of the exposure of the breeder to APEC, at the environmental and digestive levels
>Reduction of the number of APEC in the flora of the day-one-chick
>Control of the reproductive infections of the breeders
>Reduction of the fecal contamination of fertile eggs
>Improvement of the eggshell quality of the fertile egg, to prevent the entry of APEC
Vaccines do not offer complete protection due to the diversity of APEC strains. Whenever breeders are vaccinated, their offspring has efficient immunization until day 15 of age.
On the other hand, there are two kinds of vaccines intended for pullets, one with no cross-protection over APEC strains and another with cross-protection over several strains.
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Authors:
PlusVet Animal Health
Influencers who recommended :
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5 de agosto de 2022
very useful and practical information on APEC
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22 de noviembre de 2021
Any avian respiratory disease that causes ciliostasis of the respiratory tract gives APEC organisms greater opportunity to invade and wreak havoc eg Mycoplasmosis , aspergilosis etc
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2 de octubre de 2021
Where drugs are abused resistant pathogenic strains emerge. E. Coli is opportunistic pathogens. They become pathogenic when subjected to a lot of predisposing factors that already enumerated. The amino penicillins like ampicillin and amoxicillin are good threat to the pathogens. Areas where m. gallisepticum are recurrent based on poor parental and grand parental raising, makes the disease to be prevalent with disease complexed syndromes. Unfortunately, most of the revised pathogenic strains based on physical and chemical induced mutation subsists in poor sanitary drinking waters. Such strains are very difficult to treat unless with alternate medications. In good discuss biosecurity is the keyword especially in drinking water and feeds. Developing countries are worst hit by this disease because of less advanced poultry technology.
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28 de septiembre de 2021
A very good account on colibacillosis covering all the angles. Yes, ascending infection is a very high possibility especially in breeders under artificial insemination. In India, 95% of the breeding flocks are in cages under insemination. the hen is insemination on every 4th day and the instruments are used daily. if they are not sterilized and handled properly there is a risk of ascending infection for sure. many times this is manifested as egg yolk peritonitis.
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19 de agosto de 2021
E.Coli bacteria is omnipresent. it is there everywhere.
people who follow reasonable biosecurity and vaccination program for the common viral diseases know that e.coli lives with us unnoticed.
there is less scope for the presence of e.coli in borewell water drawn from 200ft and deeper, pumped to a good height and dispensed through nipple drinkers without outside contamination.
most of the feed now is steamed and dispensed through bulk tankers and mechanical feeding systems which goes to birds untouched.
sudden climate changes vaccination stress and attack of viral diseases make the bird weak and E.coli precipitates and takes an upper hand.
Farm planning and management systems should take care of sudden climate changes and proper vaccination programs besides reasonable biosecurity policies like all in all-out housing & watching the movement of men and material should keep this omnipresent GOD to be present but at its place.
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2 de agosto de 2021
E.Coli bacteria is omnipresent. we have to plan to keep it under control by reducing the further contamination through human hands. feed & water should go the birds with no human touch.
general cleanliness and good ventilation keeps the bacterial level low. variations in environment temperatures make the birds vulnerable for the infection.
Most important concerns to the veterinarian are predisposing factors like viral diseases and vaccination stress and mycoplasma. when these things are present the multiplication of e.coli is so fast that the primary treatment to kill the bacteria goes very slow.
Besides antibiotics bacteriophases are having a promising edge in the direct treatment of e.coli.
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2 de agosto de 2021
It is unthinkable that at this point in history, Escherichia coli continues to be a problem. Perhaps we have thrown antibiotics by the window prematurely, without having an effective alternative.
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31 de julio de 2021
Very good information on coli bacillosis for all involved in poultry production. Please continue the good work. Look forward to more information. It is true that contamination thru water feed litter air and in contact objects and people working on the premises cause this problem. These causes need to be identified and addressed at the management level. Treatment may or may not work, is expensive and may have to be repeated several times. Surviving birds may become carrier and may become culls or have poor performance
Biosecurity at all levels remains an effective tool.
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12 de junio de 2024
We r facing antibiotic resistant Colibacillosis in Pakistan and I got a great success against said bacteria by Esterified butyric acids.
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6 de mayo de 2024
We should also look at mutation of the E. Coli bacteria as a factor for the increased resistance to antibiotics.
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Shivaram Rao
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PhD Director Principal de Nutrición y Servicios Técnicos de Pilgrim’s Pride Corporation
United States
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