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September 4, 2020
Indianapolis, Indiana, United States

Protease enzyme and trypsin inhibitor: Novus’s results

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Dr. RAVI KUMAR AMERNENI Dr. RAVI KUMAR AMERNENI
Masters in Aquaculture
May 3, 2020

I am from shrimp farming industry. Most of the farmers are using fermented soya in the feed. Trypsin inhibitor is one of the anti nutritional factor. Can we use any protease enzyme to counter this and which product is suitable for this purpose?
Thank you

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Novus International Novus International
Missouri, United States
May 5, 2020
Dr. RAVI KUMAR AMERNENI Not all proteases are effective in hydrolyzing trypsin inhibitors. Some of them have low specificity for TIs, others are severely inhibited by TIs and hence are not effective. Cibenza DP100 is highly effective in hydrolyzing soybean TIs.
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May 4, 2020

Morning, hello there. Very interesting discussion, thanks for illustrating us. I have a question for Mercedes and Juxing; your answers, reflections, I assumed, are considering enzymes addition in dry form. What would be the effect if we would spray proteases + phytases + NSPases, let´s say more or less during a short period of time, or even at the same time over the pellet? Any experience? Many thanks! All the best to everyone.

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Mercedes Vazquez-Anon Mercedes Vazquez-Anon
Senior director of animal nutrition
Novus International Novus International
Missouri, United States
May 4, 2020
Rafael Duran Hi Rafael, our experience is with dry form of enzymes.
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May 6, 2020
Hi every one
Mercedes Vazquez - Anon
I wanted to know about flavoring compounds. Is there any product in the market for poultry, and what is the acceptance for sucrose octa acetate?
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May 6, 2020
Why is this difficult to construct matrix valves for xylanases and Nspase?
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Dr Piotr Stanislawski Dr Piotr Stanislawski
Nutritionist
DSM DSM
May 6, 2020
Sadaqat there are matrixes for NSP enzymes used by part of nutritionists/companies. In practice we use usually at least 2 enzymes: carbohydrase (xylanase and/or glucanase) and phytase. Then using matrix for phytase we add only ME uplift for cereals with carbohydrase ( eg.5-6% for wheat and/or 8% for barley).Other nutrients for carbohydrases are neglected.
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Pirzado M Zakria Pirzado M Zakria
Veterinary Doctor
May 7, 2020
How can we calculate matrix values when we use protease, phytase and NSPs in combination?
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Dr Piotr Stanislawski Dr Piotr Stanislawski
Nutritionist
DSM DSM
May 7, 2020

Pirzado M Zakria Using "cocktail" of some enzymes there is not additive effect. As the general rule, NSP are calculated as 100% for energy, phytase 100% for minerals and protease 100% for protein/AA (I mean 100% as declared). Other effect of all enzymes are calculated partly or neglected (eg. AA for NSP or energy for phytase). This is not just simple adding. Each enzyme company have own research and recommendations.
Pls ask your enzyme/premix supplier for details.

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May 7, 2020

Raquel Araujo, interesting and coherent information. However, I would like to ask a question about the nature of the protein, specifically that of soy. It is known that the gastric digestion of soy protein, produces a series of peptides with an action of importance for the health of the animal, as an antioxidant, of increasing the phagocytic capacity of macrophages, among others. Thus it can be deduced that the protein source goes beyond the simple supply of amino acids. It turns out that when we replace one protein source with another, we simply rely on the amino acid composition. And when we analyze the results, we usually associate the differences in animal performance, the possible differences in the digestibility of amino acids. This reality also has implications when we use amino acid supplementation, reducing the protein source. In this context, how is the use of proteases? I will be reasoning correctly.

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Gemma González Ortiz Gemma González Ortiz
Research Manager
May 8, 2020
A work published in 2017 (Santos et al., 2017) http://dx.doi.org/10.1016/j.aninu.2017.02.001 showed there is no additivity in the combination of phytase superdosing, xylanase and protease. Something to take into account when formulating diets and accept that having more enzymes in the formula does not mean they are going to work with the same efficiency. There is a nutrient limitation as key nutrients are digested first by the dominant enzyme leaving less space to work for other enzymes! So, it must to be accepted that working with enzymes does not mean 1+1+1 is 3!
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Amin Nahal Amin Nahal
Student
May 28, 2020
Gemma González Ortiz thanks
Reply
June 2, 2020
Dear Gemma González Ortiz, yes your are right but we can get 1+1+1=3, if we supplement enzymes with their best, and the enzymes best is to provide enough room(substrate) to enzymes. Moreover, enzymes are meant to be cost saving with sustaining performance of birds. i suggest we should follow the bell shaped curve for enzymes. reducing the nutrient specs of feed, supplement the enzymes you will notice the 1+1+1 is 3 effect. Thanks
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Guillaume Trepo Guillaume Trepo
Global Enzyme Lead, DSM Animal Nutrition & Health
DSM DSM
June 1, 2020

Thanks all for the good discussion.
I would like to confirm a few points mentioned above:

Indeed exogenous Proteases do contribute to mitigate variability in quality from SBM (and protein sources) by improving aa digestibility and degrading Anti Nutritional Factors like Trypsin inhibitors and as well as lectin. True that the benefits are all the more important that digestibility of the amino acids of the raw material is suboptimal.
As also mentioned previously, exogenous Proteases also contribute positively to Gastrointestinal Functionality by reducing the risk of development of enteric pathogens.

Furthermore, exogenous proteases have a proven compatibility and even clear additivity with other feed enzymes such as Phytases, Xylanases & Amylases.
In a recent collaboration with Massey University, New Zealand, we at DSM have also observed synergistic effect on a.a. digestibility between DSM ProAct and DSM HiPhos:
A.J.Cowieson, J.O.B.Sorbara, G.Pappenberger, M.R.Abdollah, V.Ravindran. Toward standardized amino acid matrices for exogenous phytase and protease in corn–soybean meal–based diets for broilers. Poultry Science.
Available online 25 March 2020. https://doi.org/10.1016/j.psj.2019.12.071

Best regards,

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Amin Nahal Amin Nahal
Student
June 1, 2020

Can i reduce crude protein in diets when i used protease enzyme (cibenza d100) in corn soya diets

Reply
June 1, 2020

Amin Nahal In general, yes. However, as a nutritionist, the focus needs to be on the digestibility of Amino Acids (AA) and energy value (ME) of the protease in use. It will depend on many factors mainly related to the quality of SBM used in the diets and the formulation (AA/ME ratio!) and many other factors. I will recommend you reach out to one of the R&D team members at Novus International, and they will be very happy to help you. You can also start with the Novus representative/distributor in your Region/Country!

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Amin Nahal Amin Nahal
Student
June 1, 2020

Nasser H Odetallah thanks happy eid

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Novus International Novus International
Missouri, United States
June 1, 2020

Amin Nahal Yes, you can reduce crude protein levels in the diet when you use protease in corn soya diets, but the matrix will depend on many factors including quality of soybean meal

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June 2, 2020

Guillaume Trepo, message of interest for understanding the matter at hand. However, with regard to the compatibility of the action of the protease with the different carbohydrates, exactly one of the ones that has the greatest action on the soy fiber, mannanase, was missing. The relevance of this detail is related to the fact that the fiber soybean, admittedly, has a negative effect on the swine microbiota. I believe that this enzyme may also be compatible with protease.

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