Protease enzyme and trypsin inhibitor: Novus’s results

Thanks all for the good discussion.
I would like to confirm a few points mentioned above:

Indeed exogenous Proteases do contribute to mitigate variability in quality from SBM (and protein sources) by improving aa digestibility and degrading Anti Nutritional Factors like Trypsin inhibitors and as well as lectin. True that the benefits are all the more important that digestibility of the amino acids of the raw material is suboptimal.
As also mentioned previously, exogenous Proteases also contribute positively to Gastrointestinal Functionality by reducing the risk of development of enteric pathogens.

Furthermore, exogenous proteases have a proven compatibility and even clear additivity with other feed enzymes such as Phytases, Xylanases & Amylases.
In a recent collaboration with Massey University, New Zealand, we at DSM have also observed synergistic effect on a.a. digestibility between DSM ProAct and DSM HiPhos:
A.J.Cowieson, J.O.B.Sorbara, G.Pappenberger, M.R.Abdollah, V.Ravindran. Toward standardized amino acid matrices for exogenous phytase and protease in corn–soybean meal–based diets for broilers. Poultry Science.
Available online 25 March 2020. https://doi.org/10.1016/j.psj.2019.12.071

Best regards,

Can i reduce crude protein in diets when i used protease enzyme (cibenza d100) in corn soya diets

Amin Nahal In general, yes. However, as a nutritionist, the focus needs to be on the digestibility of Amino Acids (AA) and energy value (ME) of the protease in use. It will depend on many factors mainly related to the quality of SBM used in the diets and the formulation (AA/ME ratio!) and many other factors. I will recommend you reach out to one of the R&D team members at Novus International, and they will be very happy to help you. You can also start with the Novus representative/distributor in your Region/Country!

Nasser H Odetallah thanks happy eid

Amin Nahal Yes, you can reduce crude protein levels in the diet when you use protease in corn soya diets, but the matrix will depend on many factors including quality of soybean meal

Guillaume Trepo, message of interest for understanding the matter at hand. However, with regard to the compatibility of the action of the protease with the different carbohydrates, exactly one of the ones that has the greatest action on the soy fiber, mannanase, was missing. The relevance of this detail is related to the fact that the fiber soybean, admittedly, has a negative effect on the swine microbiota. I believe that this enzyme may also be compatible with protease.

Daulat Rehman Khan
I have a similar experience. Often times non-protease products such as phytase will have a greater and more consistent uplift in amino acid digestibility than proteases. In these situations it is likely more about reduction in endogenous losses, or breakdown of complexes (phytate interactions for example).

I do believe in the POTENTIAL for proteases, but not in any commercially available product. For me this comes down to target substrate. Phytase has a well defined substrate, that is largely indigestible in the absence of exogenous enzymes. Protein on the other hand has generally high digestibility. Some individual amino acids are digested with over 95% efficiency. And this high digestibility is due to an abundance of endogenously produced proteases.

Therefore, if we are to push protein digestibilty higher we need to develop a protease that targets resistant substrates, which are not consistent. Unfortunately, all commercial proteases of which I'm aware are not designed for animal feed, but adapted from proteases developed for other applications. They are generally non-specific, and will as a result be more likely to spare the need for endogenous acitivity, than to act as a supplement to it.

Furthermore, when proteases are used with other enyzmes, I've seen data suggesting that the first substrate for these added proteases are *THE OTHER* added enzymes (Phytase, carbohydrases, etc.), thus reducing the impact of these other enzymes insead of enhancing the response.

Dear Prof. Daulant Rehman Khan

Please make sure the protease you are using is low pH gut stable. From my experience there are many so called proteases av. in the market but some of them is not pH stable. As the protease will first start function in small intestine it has to pass the stomach. From the nature point of view Proteases have the best working condition at pH 8 to 10.
Producing Feed Proteases need to take this into consideration and create them gut stable and lowering the optimum Ph.
A different thing could be your diet formulation has been too concentrated and the birds didn't need this higher digestibility and thereby didn't make use of it.
Running a trial with protease you need to be around 15 % below breeder recommendation to see the effect, that is my experience.

Rgds. Arne Korsbak

What will be the units that need to add in feed if combine with charcoal? What is the result?

Hello,
can I have the cibenza dp100 matrix?
thank you.