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Protease enzyme and trypsin inhibitor: Novus’s results

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June 24, 2020
Dr Piotr Stanislawski thanks alot sir
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Pirzado M Zakria Pirzado M Zakria
Veterinary Doctor
May 7, 2020
How can we calculate matrix values when we use protease, phytase and NSPs in combination?
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Dr Piotr Stanislawski Dr Piotr Stanislawski
Nutritionist
DSM DSM
May 7, 2020

Pirzado M Zakria Using "cocktail" of some enzymes there is not additive effect. As the general rule, NSP are calculated as 100% for energy, phytase 100% for minerals and protease 100% for protein/AA (I mean 100% as declared). Other effect of all enzymes are calculated partly or neglected (eg. AA for NSP or energy for phytase). This is not just simple adding. Each enzyme company have own research and recommendations.
Pls ask your enzyme/premix supplier for details.

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Haroon Mushtaq Haroon Mushtaq
PostDoc in Poultry Nutrition
June 8, 2020
Pirzado M Zakria Also need to know which enzyme is contributing more in that cocktail (e.g.) if its NSPase base then you can consider ME, primarily, and ignore other factors. Do likewise for other enzymes.
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May 7, 2020

Raquel Araujo, interesting and coherent information. However, I would like to ask a question about the nature of the protein, specifically that of soy. It is known that the gastric digestion of soy protein, produces a series of peptides with an action of importance for the health of the animal, as an antioxidant, of increasing the phagocytic capacity of macrophages, among others. Thus it can be deduced that the protein source goes beyond the simple supply of amino acids. It turns out that when we replace one protein source with another, we simply rely on the amino acid composition. And when we analyze the results, we usually associate the differences in animal performance, the possible differences in the digestibility of amino acids. This reality also has implications when we use amino acid supplementation, reducing the protein source. In this context, how is the use of proteases? I will be reasoning correctly.

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Gemma González Ortiz Gemma González Ortiz
Research Manager
May 8, 2020
A work published in 2017 (Santos et al., 2017) http://dx.doi.org/10.1016/j.aninu.2017.02.001 showed there is no additivity in the combination of phytase superdosing, xylanase and protease. Something to take into account when formulating diets and accept that having more enzymes in the formula does not mean they are going to work with the same efficiency. There is a nutrient limitation as key nutrients are digested first by the dominant enzyme leaving less space to work for other enzymes! So, it must to be accepted that working with enzymes does not mean 1+1+1 is 3!
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May 28, 2020
Gemma González Ortiz thanks
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June 2, 2020
Dear Gemma González Ortiz, yes your are right but we can get 1+1+1=3, if we supplement enzymes with their best, and the enzymes best is to provide enough room(substrate) to enzymes. Moreover, enzymes are meant to be cost saving with sustaining performance of birds. i suggest we should follow the bell shaped curve for enzymes. reducing the nutrient specs of feed, supplement the enzymes you will notice the 1+1+1 is 3 effect. Thanks
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Guillaume Trepo Guillaume Trepo
Global Enzyme Lead, DSM Animal Nutrition & Health
DSM DSM
June 1, 2020

Thanks all for the good discussion.
I would like to confirm a few points mentioned above:

Indeed exogenous Proteases do contribute to mitigate variability in quality from SBM (and protein sources) by improving aa digestibility and degrading Anti Nutritional Factors like Trypsin inhibitors and as well as lectin. True that the benefits are all the more important that digestibility of the amino acids of the raw material is suboptimal.
As also mentioned previously, exogenous Proteases also contribute positively to Gastrointestinal Functionality by reducing the risk of development of enteric pathogens.

Furthermore, exogenous proteases have a proven compatibility and even clear additivity with other feed enzymes such as Phytases, Xylanases & Amylases.
In a recent collaboration with Massey University, New Zealand, we at DSM have also observed synergistic effect on a.a. digestibility between DSM ProAct and DSM HiPhos:
A.J.Cowieson, J.O.B.Sorbara, G.Pappenberger, M.R.Abdollah, V.Ravindran. Toward standardized amino acid matrices for exogenous phytase and protease in corn–soybean meal–based diets for broilers. Poultry Science.
Available online 25 March 2020. https://doi.org/10.1016/j.psj.2019.12.071

Best regards,

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Novus International Novus International
Missouri, United States
June 8, 2020
We at Novus observed a similar synergistic behavior of proteases and phytases. We ran a 42d experiment to evaluate the effects of supplementation of CIBENZA® PHYTAVERSE® G10 in combination with a protease CIBENZA® DP100 in broiler chicks. It was evident that supplementing with both phytase and protease with their full nutrient values led to improved performance beyond that achieved with either enzyme supplemented separately.
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June 1, 2020

Can i reduce crude protein in diets when i used protease enzyme (cibenza d100) in corn soya diets

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June 1, 2020

Amin Nahal In general, yes. However, as a nutritionist, the focus needs to be on the digestibility of Amino Acids (AA) and energy value (ME) of the protease in use. It will depend on many factors mainly related to the quality of SBM used in the diets and the formulation (AA/ME ratio!) and many other factors. I will recommend you reach out to one of the R&D team members at Novus International, and they will be very happy to help you. You can also start with the Novus representative/distributor in your Region/Country!

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June 1, 2020

Nasser H Odetallah thanks happy eid

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Novus International Novus International
Missouri, United States
June 1, 2020

Amin Nahal Yes, you can reduce crude protein levels in the diet when you use protease in corn soya diets, but the matrix will depend on many factors including quality of soybean meal

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June 2, 2020

Guillaume Trepo, message of interest for understanding the matter at hand. However, with regard to the compatibility of the action of the protease with the different carbohydrates, exactly one of the ones that has the greatest action on the soy fiber, mannanase, was missing. The relevance of this detail is related to the fact that the fiber soybean, admittedly, has a negative effect on the swine microbiota. I believe that this enzyme may also be compatible with protease.

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Mangalmurti Pathak Mangalmurti Pathak
MVSc & A.H. Animal Nutrition
June 9, 2020
It is likely that addition of protease enzyme in feed with the reduction of protein in the feed could help in better balance between essential and non essential amino acids there by reducing the endogenous losses.
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June 18, 2020
In one of my experment, we observed that the protease has no significant improvement in the amino acids digestiblities in corn, SBM and Wheat based diet. It was an independent university research. There is a lot of discussion about the matrix values of the enzymes which are advocated by the producers. In fact, it need to be critically evaluted by the feed mill nutritionists and try to establish their own matrix for each product they wanted to use under their own raw material and production conditions.
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Joshua Jendza Joshua Jendza
Animal Nutritionist
July 20, 2020
Daulat Rehman Khan
I have a similar experience. Often times non-protease products such as phytase will have a greater and more consistent uplift in amino acid digestibility than proteases. In these situations it is likely more about reduction in endogenous losses, or breakdown of complexes (phytate interactions for example).

I do believe in the POTENTIAL for proteases, but not in any commercially available product. For me this comes down to target substrate. Phytase has a well defined substrate, that is largely indigestible in the absence of exogenous enzymes. Protein on the other hand has generally high digestibility. Some individual amino acids are digested with over 95% efficiency. And this high digestibility is due to an abundance of endogenously produced proteases.

Therefore, if we are to push protein digestibilty higher we need to develop a protease that targets resistant substrates, which are not consistent. Unfortunately, all commercial proteases of which I'm aware are not designed for animal feed, but adapted from proteases developed for other applications. They are generally non-specific, and will as a result be more likely to spare the need for endogenous acitivity, than to act as a supplement to it.

Furthermore, when proteases are used with other enyzmes, I've seen data suggesting that the first substrate for these added proteases are *THE OTHER* added enzymes (Phytase, carbohydrases, etc.), thus reducing the impact of these other enzymes insead of enhancing the response.
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Arne Korsbak Arne Korsbak
Marketing manager, Feed enzymes Europe
DSM DSM
June 29, 2020

Dear Prof. Daulant Rehman Khan

Please make sure the protease you are using is low pH gut stable. From my experience there are many so called proteases av. in the market but some of them is not pH stable. As the protease will first start function in small intestine it has to pass the stomach. From the nature point of view Proteases have the best working condition at pH 8 to 10.
Producing Feed Proteases need to take this into consideration and create them gut stable and lowering the optimum Ph.
A different thing could be your diet formulation has been too concentrated and the birds didn't need this higher digestibility and thereby didn't make use of it.
Running a trial with protease you need to be around 15 % below breeder recommendation to see the effect, that is my experience.

Rgds. Arne Korsbak

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