Rudolf Hein

Bouayad,I have def. never implicating that the insert does always interfere with HVT protection,However myself involved in RD HVT vector development have shown that in several cases depending on the construct of rHVT efficacy is less than the parent HVT. Particularly in early challenge in those cases it is recommended to add SBB 1 or CVI 988.In this forum we should focus on administration failures ...

Two different vaccination proceedings “in Ovo” and day old s.c hatchery vaccinationCan draw a list of application problems,of coarse quality of vaccine (e.g PFU titer etc) May play a role

MD vaccination failures may occur due to several factors This will also an important fact in the failures of the response to the immune foreign gene inserts. using recombinant HVT vector vaccines

Puneet Jindal You have to be carefully in combining different type of activated vaccines are not the antigens but the emulsion(Adjuvant) be may not compatible, What do you mean with:" after gap of 40-50" ?

Puneet Jindal Yes no problem there will be no interference .

Puneet JindalI am not related to CEVA but according me there would be no any problem to combine the rHVT/H5 with SB1 .

I agree with comments made by Michel Bublot Mixing any recombinantHVT vector vaccine with a conventional HVT vaccine will interfere with the protection of the insert like in your case with the AI/H5 protection As mentioned will not effect the MD protection To improve protection of the recombinant for MD you can if manufacture recommend to combine the recombinant HVT vector vaccine with SB1 or Ris ...

Karel A Schat yes manuscript has been submitted

Regarding recombinant vector CIAV vaccine construction rHVT to be used as a hatchery vaccination , no success yet, inserts expression cassettes VPs , no in vivo expression.Investigation using the CRISPR/Cas9 tool may be a possible solution to generate in the future rHVT/CIAV.

Very useful, excellent