The interaction between betaine and crude protein and broiler feed

Dear Mr. Dubois, thank you for your comment! Please be informed that the lowered CP treatments (starter and grower) were not deficient on methyl groups, comparing to the normal CP groups (starter and grower). For example in the lowered CP group in the starter phase, methionine was supplemented in higher amount, having a higher level on dMet 5,57 g/ kg, comparing to normal CP treatment with dMet 5,33 g/ kg. The dM+C level was equal 8,4 g/ kg in both (normal CP and lowered CP), also the d.M+C/ d.Lysin ratio was the same in both 0,7; and with the same level on Choline in both starter groups. The lowered CP diet was deficient in d.G+S level. The same situation in the grower groups, with adapted levels for the grower phase. Fact is, that betaine as TMG donates its one methyl group in the Hcy- Met cycle, The second and third methyl groups of betaine, will form DMG-> Sarcosine-> Glycine (as shown in the fig.1 according to Craig, 2004); therefore the affirmation, that indirectly betaine is also a potential source of glycine, supported by the dG+S levels. As far as I know, in the EU is no authorized feed grade glycine product available, but it is a good idea for a future trial to include a glycine group. Thank you and best regards, Ana P.S. I have a MSc degree in zootechnical engineering ??.