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Life Rainbow
Annual survey of mycotoxin in feed in 2018-Taiwan
Published: May 22, 2019
By: Life Rainbow
In 2018, 430 randomly collected feed samples of raw materials and feed mills in farms were analyzed. Samples were tested for aflatoxins (B1, B2, G1, and G2), zearalenone, fumonisins (B1, B2, and B3) and deoxynivalenol by the ELISA Mycotoxin analysis kit (Romer Labs®).
Results
Of 430 feed samples collected, 90.9% were contaminated with deoxynivalenol (table 1). The maximum concentration of deoxynivalenol was over the limit of quantification in this survey.
Data on corn meal, nursery, and pregnant diet were conducted to give the prevalence of mycotoxins. Out of total 430 samples, 84 samples from corn meal were analyzed that observed 84.5% of feed were contaminated with deoxynivalenol (table 2).
Of 111 samples of nursery feed collected, 91.0% of samples were contaminated with deoxynivalenol (table 3). The occurrence of mycotoxin in pregnant diet also analyzed. Deoxynivalenol and aflatoxins were most prevalent mycotoxins in pregnant diet, detected in 91.9% and 80.5% of samples respectively. (table 4).
Conclusion:
In 2018, the most prevalent mycotoxin in Taiwan was deoxynivalenol, detected in 90.9% of samples including raw materials and finished feeds. Aflatoxins were found in more than 50% of samples in 2018, which showed higher occurrence compared to previous year. Co-occurrence was also observed in this survey. A full of 79% samples contained at least two or more mycotoxins, that may give synergistic complications on animal performance and immunity that cause financial loss of producers. Mycotoxin contaminated feedstuffs will raise the risk to impact animal production and economic profit. According to the reginal survey, feed manufacturers and animal producers can be aware of the occurrence of mycotoxins and implemented actions into mycotoxin management.
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9 de julio de 2019
I agree that it needs to be validated with traditional methods as stated in my earlier comments, but there are companies that have validated their tests kits for feeds including raw materials. There are exception that some foreign material may interfere with the test, but again, for field testing is one of the best methods, since there isn’t any traditional method to handle in the field.
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Alltech
9 de julio de 2019
ELISA is not validated for finished feed, we do not even recommend ELISA for testing of finished feed. Never less, it is the best method for testing raw materials. You can always validate your ELISA equipment and method against reference methods as LC MS/MS or HPLC.
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8 de julio de 2019
Dear Rena. I have been working with ELISA since the 1980’s, I understand that you may find some false positives, but they are better than false positives and make you recheck the sample with traditional methods. Remember, that in the field, ELISA’S are the most reliable of test kits that we can despond on to either buy grain, feed, other materials.
I have worked I Philippines many times and I understand your dilemma, but in reality, you should rely on these tests. There are many ELISA kits in the market and I like to know if you used any of the gold label antibodies rapid tests or you use the conjugate, competition for the antigen bound to the plastic.
Best of luck and do rely on these tests.
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9 de julio de 2019
It is necessary to use the lc ms/ms method because it is more accurate as well as multi-mycotoxins and not a single mycotoxins
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8 de julio de 2019
Depends on the kit assay. Sometimes screening kits may provide false positive reactions. But you can use positive and negative controls along with calibration standards and QC for more accurate results. You can also compare with cross reference method of LC-MS/MS which is more expensive. Hope this will be helpful.
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8 de julio de 2019
The results should be fairly accurate since Elisa is antigen-specific. Interference from other components in the substrate is unlikely.
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Ariela Marketing Co
2 de julio de 2019
How accurate is testing done in finished feeds with ELISA? Does not the different components affect the accuracy of the test? Thanks!
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