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Claudio Afonso likes the comment:
Claudio Afonso Thanks for your comment! We used the 16S RNA amplicon sequencing analyses. I've attached the link for the papers related to this research. If you would like to take a look at the overall results. https://www.frontiersin.org/articles/10.3389/fphys.2020.00020/full https://www.frontiersin.org/articles/10.3389/fmicb.2019.02858/full
Participation in Forum on February 28, 2020
Nice work. Wondering if you are planning to try using just total RNA random sequencing instead of 16S variable region. In our hands when we use RNA, we obtain almost complete 16S, 18s and 23S for species level resolution and in some cases and viral RNAs as well. Since you are dealing with RNA random amplified you may obtain a sense of what bacteria/viruses are transcriptionally active.
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Participation in Forum on February 28, 2020
Nice and important work! I am wondering if you are using metagenomics the 16S variant region of Ribosomal RNA or just random sequencing of total RNA. This last one because it may provide specie resolution for bacterial ribosomal in addition of information on viral microbiota including bacteriophages.
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Denise Russi Rodrigues (USDA, Ohio State University) talked about probiotics and early colonization as means of improving gut health and poultry performance, during IPPE 2020 in Atlanta, USA.
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Introduction Significant economic losses in the poultry industries due to moderate to high mortality and decreased egg production have resulted from H9N2 low pathogenic avian influenza virus (LPAIV) infections across North Africa, the Middle East and Asia (Fusaro and others 2011, Lee and Song 2013). The currently circulating Eurasian H9N2 LPAIV has rapidly spread to become the most prevalent LPAI ...
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