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How should we evaluate soybean meal quality?

Ureatic activity and protein solubility in KOH of soybean meal samples obtained in poultry feed mills

Published: April 23, 2020
By: Mihayr Morais Jardim 1, Emmanuel Arnhold 2, Fabyola Barros de Carvalho 3, Roberto de Moraes Jardim Filho 4, Jose Henrique Stringhini 5. / 1 Nutrial Insumos Agropecuários; 2 Departamento de Zootecnia, EVZ/UFG, Brazil; 3 Departamento de Zootecnia, EVZ/UFG, Brazil; 4 São Salvador Alimentos; 5 Departamento de Zootecnia, EVZ/UFG; CNPq, Researcher, Brazil.
INTRODUCTION
The use of soybean meal in poultry diets can be tested for antinutritional factors, which is easily identified with ureatic activity and protein solubility in KOH analysis. This research aimed to evaluate soybean meal quality measured by ureatic activity and KOH solubility in poultry feed mills.
MATERIAL AND METHODS
For this analysis, 283 samples were obtained from a broiler feed mill located in Goiás, Brazil. Soybean meal samples were categorized by the KOH protein solubility in 5 groups: < 75%; 75-80%; 80-85%; 85-90% and > 90% and 5 other categories regarding ureatic activity: 0; 0.01; 0.011-0.05; 0.051-0.1 and > 0.1. Descriptive statistics was used to evaluate results.
 
RESULTS
The percentage of samples obtained were classified in 5 categories of protein solubility in KOH (%) and are presented in Table 1.  For the categories of Ureatic activity index (Δ pH), the percentual values found for each of the 5 categories evaluated and are presented in Table 2. For protein solubility in KOH, it was observed that 60.41% of the samples analyzed presented levels varying from 75 to 85% of protein solubility in KOH, that could be considered as a secure index to identify any change in soybean meal processing.  But, it is necessary to pay attention to the 39.59% of these samples that the results were outside of the safe range. On the other hand, 87.0% of soybean meal samples had an ureatic activity index lower than 0.1 ΔpH, which can be considered adequate for chickens. But it is important to consider that 13.0% of these samples can be classified as low quality samples. Another value that draws attention is 10.25% of samples with ureatic activity value higher than 0.1 Δ pH and protein solubility greater than 85%. Another value that draws attention is 10.25% of samples with ureatic activity value higher than 0.1 Δ pH and protein solubility greater than 85%. This number of samples exceeding 10% means the possibility of reducing the ingredient quality soybean meal and eventual hazards in broiler performance when this ingredient is added to diets.
CONCLUSION
it is important to investigate the possibility of errors in sampling or analysis to ensure effective quality control of the ingredients.
Ureatic activity and protein solubility in KOH of soybean meal samples obtained in poultry feed mills - Image 1
 
Ureatic activity and protein solubility in KOH of soybean meal samples obtained in poultry feed mills - Image 2
Published in the Proceedings of the 2020 International Poultry Scientific Forum, p. 101, abstract P330. Presented at IPPE 2020.

Burnham, L.L. et al. Effects of sodium sulfate and extrusion on the nutritional value of soybean products for nursery pigs. Asian- Aust. J.Anim.Sci., 13:1584-1592, 2000.

Liener, I.E. Implications of antinutritional components in soybean foods. CRC Critical Reviews in Food Science and Nutrition, 34(1):31-67, 1994.

LIMA, M.R. et al. Atividade ureática. Rev. Eletr. Nutrit., 145(5)1606-1611, 2011.

SERRANO, M.P. et al. Influence of feed form and source of soybean meal of the diet on growth performance of broilers from 1 to 42 days of age. 1. Floor pen study, Poultry Science, 91(11):2838–2844, 2011 https://doi.org/10.3382/ps.2012-02371

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Authors:
Fabyola Carvalho
José Henrique Stringhini
Universidade Federal de Goiás - UFG
Universidade Federal de Goiás - UFG
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Markus Wiltafsky
Evonik Animal Nutrition
Evonik Animal Nutrition
12 de octubre de 2022

Dear Luis,
in the last few years, we had a close look at the quality of different protein-rich feed ingredients and we developed NIR calibrations which can now be used locally to estimate the quality. The reactive Lys to Lys ratio is a good indicator for over-processing and it works in Soybean products, but also in Canola Meal and DDGS. In Canola it might be worth to check in addition the levels of Glucosinolates and Erucic acid. It should be low, but it's better to check it.
All the best,
Markus

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Luis Fernando Vergamini Luna
Opta Alimentos e Insumos
27 de septiembre de 2022
Thanks a lot for the great discussion. Especially for Dr. Nelson Ruiz. Nowadays we also have some different sources of protein, besides Soybean Meal still the main one and varies from 44% PB and going down to 48% PB. These different sources like DDG, Canola, and SPC are also variable. How to correctly measure and check their quality in the plant. KOH for Canola Meal seems to be different from Soybean meal, maybe KOH 0,05. The DDG initially with high moisture, is dried with lower control on checking the effect on protein quality. SPC, that is a Soybean meal without molasses, which would be a fast check when receiving at the feed mill? Thanks, gentlemen!
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Jose Henrique Stringhini
Universidade Federal de Goiás - UFG
Universidade Federal de Goiás - UFG
17 de septiembre de 2022

Dear friends
thanks for the discussion, I am very impressed with the quality of the information. Recently, we have tested different soybean meals available in our region, and it was possible to see this difference including AME, AMEn and other digestibility variables. This makes very important the information presented by Dr. Ruiz. This makes me more interested in continuing the studies about soybean meal quality.
Prof. José Henrique Stringhini (UFG, Brazil).

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Alfredo Irazusta
Alinat
16 de septiembre de 2022

As always Dr. Nelson Ruiz is very clear in his explanation of methods on how to measure SBM at industrial process. By the way, I do not think that a very short time ( less than 14 seconds ) in the conditioner before pelleting could affect protein solubility.

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Alfredo Irazusta
Alinat
16 de septiembre de 2022

As always Dr. Nelson Ruiz is very clear in his explanation about SBM and how to measure correct industrial process. By the way a very short time about 14 seconds resident in the conditioner (before pelleting) is unlike to affect solubility of the protein. Alfredo Irazusta , Alinat SRL. Argentina.

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dan hofer
26 de agosto de 2022
We got own roaster urease was .02 KOH was 77 birds seem to like it they get 1.56 Fcr
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Mario E Tobar Jaramillo
24 de agosto de 2022

Nelson, good morning. Excellent your explanation. Best regards

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Luis Fernando Vergamini Luna
Opta Alimentos e Insumos
23 de agosto de 2022

Gentlemen, thanks for the excellent discussion.
Going deeper, the KOH solubility evaluation is based on how much the protein was exposed to a Maillard reaction. Urease activity is an indirect analysis of how protease has been affected in the thermal treatment. Reading the comments seems to be correlated, but are different evaluations, or not?

Regarding the Maillard reaction, how much the protein fraction of feed could be impacted by an expander before pelleting, could it be a relevant issue?

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Nelson Ruíz
Nelson Ruíz Nutrition LLC
Nelson Ruíz Nutrition LLC
3 de junio de 2020

Dear Dr. Stringhini et al., in my assessment your data on soybean meal quality are very interesting. Based on our experience, and considering also several reports in the scientific literature, the range of adequacy for KOH protein solubility in soybean meal is in the range of 80-85%. Your data based on the analysis of 283 samples from the commercial reality show that 52.64% of the samples or 189 out of 283 analyzed are exactly in the range 80-85%. On the other hand, 68.64% of the samples or 210 out of 279 analyzed displayed a urease activity at or below 0.05 pH units. Since 2012 (Ruiz, New insights on the urease activity range for soybean meal: A worldwide opportunity for the poultry industry. Arkansas Nutrition Conference) we have demonstrated that for solvent-extracted soybean meal (not for full-fat soybeans) the urease activity range consistent with tolerable levels of trypsin inhibitors for broiler chickens is the range 0.000-0.050 pH units. It is likely that the 36.39% of the analyzed samples that resulted in KOH protein solubility above 85.1% may somehow correspond with the 30.74% of the analyzed samples that displayed urease activity above 0.05 pH units. All these comments are, of course, approximations. Now, it is important to realize that there is also 10.95% of the samples that tested below 80.0% KOH protein solubility which may eventually be compromised as overprocessed (defining "overprocessed soybean meal" as containing less than 88% in vivo digestible lysine). Therefore, all in all, in my assessment 52.64% of the samples most likely are within the estimated adequate specs of 80-85% KOH protein solubility AND less than 0.05 pH units of urease activity (which in turn means being in the range of approx. 1.65-2.35 mg of trypsin inhibitors/g, or approx. 3.0-4.0 TIU/mg) assuming that the 52.64% from the KOH solubility evaluation is contained within the 68.64% of urease activity values below 0.05 pH units. For practical purposes this show the importance of ADJUSTING FORMULATION for specific soybean meal lots correctly evaluated for their quality parameters within the concept of precise nutrition. Nelson Ruiz Nutrition, LLC, Suwanee, GA, USA.

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Syed Farjad Zaidi
16 de octubre de 2022
Unlocking the different parameters to know the exact matrix of soya and other meals.ad vomment by Markus NIR evaluation is fast correct way to assess quality. Higher inclusion rate of meals could make a negative effect if the quality varies, in current days and time to come as the price reduction is not in sight.
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