Dear,
the safety of the vaccination was evaluated looking at clinical signs in all groups every day for 11 days (the end of the trial).
The aim of our paper was to evaluate the spreading of the vaccine during standard operation at the hatchery where, in some cases, chicks need to be vaccinated with different vaccines and also transported together with animal vaccinated with different vaccines. For this reason, we keep the different vaccinated chicks in contact for 5 hours.
In the field in the same flock vaccinated birds could stay for longer time in contact with unvaccinated birds (it's difficult to reach 100% of vaccine coverage) and so the spread of the vaccine could, of course, be different.
I hope to answer your question and if you need more clarification do not hesitate to contact me.