Elisa report interpretation
Greetings. I would be grateful if you could interpret below Elisa report about a broiler breeder 46 week vaccinated on date 12/02/2022 with Ma5 and clone 30 spray. This farm is composed of 5 pens. I would like to know:
1- How can I specify validity of internal controls of the Elisa report. Is it necessary to be similar to that of certificate of analysis? If no what is the difference between them to be accept or shall I depend on the reader to validate it.
2- All the pens have the same mean titer and zero %CV although the mean titer within the expected range.
3- Why does that phenomena occur and what is the corrective action? Thanks and regards
Elisa report Mean titer 18937 Titer range reference control cr100 RF16 6000-12000 Min-max titer 18937-18937 Mean titer Ref Control 6983 GMT 18937 Internal controls .199 and .241 for negative %CV 0 .854 and .908 for positive control NO of samples 18 Raw OD for all samples 4.000 Neg/su/pos 0/0/18 Titer Group 11 Result positive
First of all mention
Is it ELISA of ND or IB? also mention name of Kit used for ELISA?
Then mention no of vaccine shots done from day one to 46 weeks of age, for which ELISA is required.
After that CV%age zero for 18 samples doesn't look possible, as same value for 18 samples is not possible practically.
Bacillus amyloliquefaciens CECT 5940 (Ecobiol®) alone or in combination with antibiotic growth promoters improve performance in broilers under enteric pathogen challenge
Respected Sir
if there is something wrong in protocol or reading that may be indicated by the reader. How can that happen since result was saved as usual?
I.B Elisa titers 18000 indicate exposure to disease.
Vaccine protective titers are around 7000.
Coefficient of variation (C.V%) is never negative and anything below 20% is considered good.
The results should be rendered back to the concerned lab. For clarity.
Test again elsewhere.
