Engormix/Poultry Industry/Technical articles

Influence of Iron Source (Organic vs Inorganic) on Broiler Performance Under a Pathogen Exposure

Published on: 8/21/2019
Author/s : Jack Garrett 1, Greg Nunnery 1, James McNaughton 2. / 1 QualiTech Inc., Chaska, MN, USA; 2 AHPharma, Inc., Salisbury, MD, USA.
Summary

Iron is a trace mineral supplemented in broiler diets. Bacterial pathogens, such as E. coli, C. perfringens and E. acervulina have an essential requirement for iron such that they have multiple sequestering mechanisms to obtain iron. This study was designed to evaluate the hypothesis that an organic form of iron (SQM Iron®, QualiTech, Inc.) could limit the availability of iron to such pathogens and reduce their impact on broiler performance. A total of 2496 mixed sex broiler chickens (Ross 708) were randomly assigned to one of 48 pens (12 pens/replicate). Diets (corn-soy based) were formulated to meet industry standards for a Prestarter (0-7d), Grower (8-35d) and Finisher (36-42d) program. Treatments included 1) iron sulfate as the sole supplemental iron source (IONC), 2) SQM Iron instead of iron sulfate (OINC), 3) IONC receiving a pathogen exposure on day 7 (IOPC), and 4) OINC receiving a pathogen exposure on day 7 (OIPC). Broilers received Cocci-Vac day 0. Sub-clinical exposure was provided by obtaining used litter containing E. acervulina (>50,000 oocysts/bird), C. perfringens (>104/bird) and E. coli (>106/bird). Performance was measured every 7 days; lesion scoring, and bacterial counts were done on day 21 and 42. Broiler growth rate and efficiency was significantly (p<0.085) impaired by the pathogen exposure every week of the study. Iron source had a significant (p<0.05 improvement on weight and efficiency through week 3 with the organic iron source. Week 4-6 had strong trends for organic source improving bird weight and efficiency. Bird mortality showed a significant (p<0.05) interaction between source and expose with those bird receiving the organic source reducing mortality under the exposure conditions. Lesion scores were significantly (p<0.01) reduced by feeding the organic iron source (exposed or unexposed). Iron source had no influence on E. coli counts. Feeding the organic iron source significantly (p<0.01) reduced C. perfringens and cocci counts at all times of the study. Results of this study showed that feeding SQM Iron as an organic iron source reduced the growth rate of pathogens. This reduction in growth rate appears to be due to limiting the accessibility of iron for growth of pathogens.

Introduction: Pathogens and Iron Requirement
  • E. coli accumulate large amounts of iron via multiple transport systems including enterochelin transport system (McIntosh and Earhart, 1977).
  • Three tetracyclines were found to possess iron-chelating activity as part of the antibiotic activity. (Greinier et al., 2000)
  • Clostridium perfringens produced larger colonies, with…ferredoxin, than that on the control medium. (Osman et al. 2013)
  • Proposed that increasing iron concentration may thus lead to an increase in E. coli growth. (Appenzitter et al. 2005)
  • Research showed that maximum growth for E. coli occurred at 0.6 mg. ferrous ion/l. medium. (Pappenheimer & Shaskan, 1944)
  • E. coli growth was not affected by increases beyond 0.6 mg/l up to 90 mg./l. (Fuchs and Bonde, 1957)
  • Numerous studies have assessed the potential viability of iron-chelators as therapeutic agents against various microbes…. (Thompson et al., 2012)
Influence of Iron Source (Organic vs Inorganic) on Broiler Performance Under a Pathogen Exposure - Image 1
 
Influence of Iron Source (Organic vs Inorganic) on Broiler Performance Under a Pathogen Exposure - Image 2
 
Influence of Iron Source (Organic vs Inorganic) on Broiler Performance Under a Pathogen Exposure - Image 3
Objective
Determine the influence of iron source on broiler performance when exposed to contaminated used litter.
Materials and Methods
  • 2x2 factorial arrangement of treatments (Iron source x bacterial exposure)
    • Iron sulfate vs SQM Fe; 20 ppm
    • Control litter vs contaminated litter
  • 2,496 mixed sex broiler chicks
    • 48 pens total (52 chicks/pen)
  • Starter (0-7 day), Grower (8-35 day), and Finisher (36-42 day).
  • Mash form were based on corn and SBM.
  • 42-d study, measurements 7, 14, 21, 35 and 42 days.
  • The sub-clinical exposure (built-up litter and moderate stress conditions via bacterial and coccidial exposure), relating to age of litter (a minimum of three previous flocks), was obtained with built-up litter, containing a mixture of coccidia E. acervulina (>50,000 oocysts/bird), Clostridium perfringens (>104 per bird) and E. coli (>106 per bird) bacteria were administered into the litter on Day 7.
Influence of Iron Source (Organic vs Inorganic) on Broiler Performance Under a Pathogen Exposure - Image 4
 
Influence of Iron Source (Organic vs Inorganic) on Broiler Performance Under a Pathogen Exposure - Image 5
Conclusions
  • SQM Iron maintained the performance of broilers when exposed with microbial pathogens.
  • The mode of action appears to be providing a highly bioavailable source of iron to the broilers while reducing the ability of microbial pathogens to utilize that same iron source for growth.
  • Therefore, to reduce the impact of a subclinical microbial infection include SQM Iron in your trace mineral fortification program.
Presented at IPPE 2019 in Atlanta, USA.
 
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