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Phileo by Lesaffre
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Phileo by Lesaffre

Effect of selected yeast fraction on the growth of Clostridium perfringens: Quantitative determination of growth inhibition and adsorption capacity

Published: August 11, 2020
Summary
To provide in vitro evidences on the antimicrobial effect of yeast cell wall (YCW), the effectiveness of YCW fractions in inhibiting the growth of several C. perfringens strains was quantitatively determined. The bacterium was grown in the presence of different YCW fractions at different concentration levels. The effect of YCW fractions on the growth parameters was analyzed. One product out o...
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Authors:
Alain Riggi
Phileo by Lesaffre
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Ruth Raspoet
Phileo by Lesaffre
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Virginie Marquis
Phileo by Lesaffre
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Mike Stahl
15 de septiembre de 2023
Who is the western canadian rep for lesaffre

thanks
Alain Riggi
Phileo by Lesaffre
15 de septiembre de 2023
@Mike Stahl
Hi
Our contact in Canada is Ron Gauthier
R.gauthier@phileo.lesaffre.com
Kind regards
Alvaro Dubois
Cargill
21 de septiembre de 2023
Interesting evaluation. There is a published paper with this evaluation?
Luc Goethals
Sanluc International nv
26 de octubre de 2023
Indeed, a new and quite particular approach. I would also be interested to receive the full study or publication (luc@sanluc.be). The main question will raise how appropriate such IN VITRO data are for the field and in VIVO. Since it's extensively described that especially for Clostridium perfringens, data from in vitro studies have poor or no value for in VIVO, because Clostridium perfringens is anaerobic, houses in the distal part of the intestine and under particular conditions (interaction with host , microbiota and diet), impossible to mimic in the laboratory. Low M.I.C. values in a petri dish, might mean nothing in the animal.
I wonder if I read the numbers correctly (will verify in the detailed study), but 1,25 mg/ML would mean 1250 ppm, which is very high. If 104 cells are adsorbed by 1 mg of product, so LOG 2, then for a "normal" contamination of Clostridium Perfringens in the gut of LOG 8, (per gram intestinal content), one would need 1 KG product ?
Furthermore in the distal part of the intestine, around LOG11 "clostridium" types are present, many beneficial clusters, few pathogenic. How selective is the product to bind only the pathogenic ones ?
Thanks for the clarification and eventual correction.
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