Mastitis in buffalo

Mr Fabiola
The treatment with tri-Sodium citrate is equally effective in cows rather in other milk producing species too. This is due to the fact that the synthesis and production of milk is remarkably similar in all these species. 
Dr Dhillon and Dr Jasmer

Dr Neelesh Sharma
Your views on finding control measures for mastitis through molecular and stem-cell research appear to be well thought of but treatment with tri-Sodium citrate also works at the molecular level. The treatment of mastitis with tri-Sodium citrate in dairy animals was first time reported by us in 1989 followed by some more publications (see references in our paper above). Further, you have rightly pointed out the irrational use of minerals and vitamins in animal as it might result in toxicity.Moreover, the imbalance of trace minerals like copper, zinc, selenium, iron etc., has already been vastly reported in the literature extant for which we need to be careful. 

Dr Jasmer Singh

Dear Sir
I know use of Tri-sodium citrate in India started from PAU, Ludhiana, thatswhy it is very frequently using in Jammu also. No doubt it has good results as supportive therapy in mastitis particularly during antibiotic therapy in the field even some times we used as such juice of citrus fruits. Everybody know that each chemical or element works at cellular level and target cell signaling for the its action. Therefore, as I mentioned that its (use of Tri-sodium citrate) not new findings because it is most commonly using in India since last very long time (or since your discovery). But now need to findout some more effective solution for mastitis. No doubt developing countries are trying lot to findout some suitable solution for the control of mastitis. Even recently Merk comp in Netherland working on "Evasion molecules" to get success in mastitis vaccination. Although mastitis is non ending field of research............

Dr Neelesh Sharma

We would like to put the things straight regarding the use of tri-sodium citrate alone for the treatment of 'acute, subacute, recurrent and even resistant to currently used antimicrobials. This salt is not used as a support for antimicrobials rather it is solely best curative measure in comparison to antibiotics/sulpha drugs etc., in controlling most commonly encountered cases of mastitis in dairy animals ( cows and buffaloes). Moreover, this treatment (tri-Sodium citrate) alone has been recorded to be the best in restoring the normal pH, biochemical constituents and alleviation of clinical signs of mastitis in affected animals. Treatment with this salt is versatile due to the fact that it preserves the normal milieus of the udder in which the commonly isolated environmental organisms ( E. coli, Strept. Staph. etc) can not thrive. See refs.

Dr KS Dhillon and Dr Jasmer Singh ( Rtd. Profs. PAU ,Ludhiana)

Dr KS Dhillon and Dr Jasmer Singh (Rtd. Profs. PAU, Ludhiana).

Dr Dhillon and Dr Jasmer ( Rtd. Profs.PAU Ludhiana).

Dear Dr.
Practically and Personally I do not agree with the statement that only Tri-sodim citrate is enough for the treatment of mastitis in bovine because I have used in so many cases. If I have worked on the topic, after that I do not believe on reference or publications. I have many papers even not only in mastitis they are showing good results although practically results are Zero. Even you can find many papers on Homeopathy treatment in mastitis and showing high efficacy, while on real ground efficacy is ZERO.
Conclusion is that I do not want to discuss more on this topic because I have enough knowledge on this aspect particularly in India. I now very well about mastitis situation and its treatment in field conditions.
Thanx for sharing your knowledge and views.

The article about mastitis therapy in buffalo is good and the cost of treatment is cheap/economical

Hi WH You have got the right formulae of tri-Sodium citrate i.e., Na3 C6 H5 O7 . 2H2O. Pl note whenever you use this salt for mastitis treatment in dairy animals inform me about the results. We can reschedule the medication dosage. Kindly do reply.

with regards Jasmer

Dear Dr Singh,
Thanks a million & one for your rely.
I am very interested in using this salt for the treatment of mastitis in dairy cattle.
The option of using a non antibiotic to treat dairy cattle mastitis is very appealing to me.
It would be an honour to be able to inform you of the results.

One other question, can you provide some information about how to prepare the sterile 5% solution in saline for intravenous administration?

Thanking you advance, I look forward to your reply with interest.

wh
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Dear Dr Singh,
Thanks for your reply.
The solution would be prepared, as per the method you provided..
The results of treatment of cows with clinical mastitis would be communicated to you.
Looking forward to having interaction with you on this activity.

Many thanks and best regards.

wh.

HI WH -Pl see that you record the pH of milk on the spot with graded pH papers or with a pen-pH meter. The ideal thing would be to estimate the citrate content in milk before and after treatment to grade the severity of the disease. These parameters can be recorded daily to establish the extent of disease and duration of cure with/without any complication from the treatment. Moreover, the procedure of grading the severity of this disease is explained through pictures in our article in Engormix.com. The treatment scedule is 50ml of the above sol. I/V morning and evening which can be repeated or followed by oral administeration of 30gms of tri-Sodium citrate in ~100ml of water.daily till recovery.

The other biomarkers like- consistency, flakes,color , presence of blood, smell, condition of udder/teat/ quarter etc., are good parameters for determining the progress of the treatment. Any other criterion which you may deem valuable in this process can be included as per your fecilities available ar hand.

With regards Jasmer

Dear Dr Singh

The treatment of initial mastitis case with sodium citrate will begin today. Oral administration would be the method employed.

When the sterile IV solution become available, treatment would be IV for the first day follwed by oral treatment, if / as required. I would inform you of the result of this inituial case.

In an previous communication you stated " We can reschedule the medication dosage. Kindly do reply. "

My question is:-
Are there other suggestions for treatment apart from those reported in the article (ie 12.5 grams or 30 grams given once daily by oral route and the twice daily Intravenous administration of tri-Sodium citrate as 5% in normal saline)?

I look forward with interest to your reply.

wh