Essential Oil-Based Nanoparticles as Antimicrobial Agents in the Food Industry

Q: What are the advantages of encapsulating essential oils (EOs) in nanosystems for food industry applications?

The application of EO encapsulated by nanosystems should offer a set of advantages that ensures its use as promising nanostructured formulations in FI application. The use of EOs in the food chain is beneficial to inhibit microorganisms contaminations, which justifies its use.

Q: What regulatory aspects and challenges are associated with the use of nanotechnology-based products in food and feed industries?

According to Brazil, a robust international regulatory framework for the evaluation of nanotechnology-based products is necessary for approval of food and feed containing nanoparticles. Despite the advances already reported, there is no standard protocol for the toxicity testing of nanomaterials that assesses, for instance, the NP excretion mechanisms after digestion. There are still no global regulations for manufacturing, labeling, or in vivo and in vitro toxicity testing, nor for potential environmental harm from nanoparticle residues.

Published: August 19, 2024

By: Micaela Guidotti-Takeuchi 1, Lígia Nunes de Morais Ribeiro 2, Fernanda Aparecida Longato dos Santos 1, Daise Aparecida Rossi 1, Flávia Della Lucia 3 and Roberta Torres de Melo 1.

Summary

Author details:

1 School of Veterinary Medicine, Federal University of Uberlandia, Uberlandia 38402-018, Brazil; 2 Institute of Biotechnology, Federal University of Uberlandia, Uberlandia 38405-320, Brazil; 3 Nutrition Faculty, Federal University of Alfenas, Alfenas 37130-001, Brazil.

1. Introduction

Recently, the interest in “green food products” based on natural compounds and minimal processing of edible products [1,2] has gained attention in determining products’ acceptance [3]. These characteristics are crucial factors that reflect the current trends and economic growth in this sector [4], which is estimated to exceed USD 13 billion by 2024, according to reports from Global Market Insights, Inc. (Selbyville, DE, USA) [5]. From this point of view, researchers and the food industry (FI) have been looking for sustainable alternatives, such as the use of essential oils (EOs), to replace synthetic additives [6], such as sorbic, benzoic, propionic acids, and sodium diacetate preservatives [7].

Despite that the microbiological control of contamination in fresh or processed foods is a topic of great interest, this is still a challenge for safe food-based systems developments. There are some pathogens that can cause food spoilage and affect nutritional and sensory qualities, as well as causing foodborne infections and toxicosis. Food contaminated with pathogenic microorganisms causes food spoilage, with consequent economic losses and undesirable effects on product quality and safety [8]. Recently, a report by the Centers for Disease Control and Prevention (CDC) (2021) demonstrated an increase of foodborne diseases related to microbial contamination during the periods 2004–2008 and 2015–2016, by nontyphoidal Salmonella, with around 159,000 and 222,000 infections, respectively [9].

Resistance modeling studies estimate an increase in resistance for priority antibiotic bacterial combinations of 17–31% between 2000 and 2030 for 52 countries, including hotspots related to Brazil [10]. With this current scenario, a thorough analysis of changes and health impacts will help to establish goals and priorities for infection prevention [9]. More efforts in differentiated approaches to ensure and maintain food safety are urgently needed.

The EOs are plant-derived compounds with complex aromatic structures and high volatility. The volatilomes [11] are presented as the total fraction of strong-smelling molecules produced in specialized plant cells (oil cells, ducts, or glands) [12]. They are secondary plant metabolites, which play essential roles in the dynamics of plants with their habitat through protection against pathogens and attraction of polarizers [13]. They can be extracted from all parts of plants (from leaves to roots) by using different methodologies. The presence, yield, and chemotype of EOs are influenced by many climatic and plant nutritional factors, as well as stress conditions. For commercial EO production (where increases in the yield are desirable), breeding and selection programs are implemented as a measure to foster specific compositions [14]. The structural profiles of commercial EOs have been previously provided by gas chromatography and mass spectrometry analysis [8,15,16]. In general, their molecular structure reveals two or three major compounds, corresponding to more than 20% of the full molecule, usually responsible for their biological properties [17–19].

The EO bioactive compounds present simultaneous antibacterial, antifungal, antiprotozoal, and antiviral properties. Synergistically, the association of Eos with conventional antimicrobials can result in the improvement of the antimicrobial activity, especially useful for prevention and control programs [20]. These plants also comprise an important part of the human diet, and are used to enhance the flavor, color, and aroma of foods [21], potentially providing safer natural preservatives alternatives [21,22] over synthetic chemical preservatives [23]. In addition, phytogenic additives are vegetal compounds or ingredients incorporated in animal feed that aid in the performance and health of animals and, consequently, to the quality of animal and plant-derived foods [24,25].

However, EOs show a set of undesirable physiochemical properties that prevent their widespread use. They are highly volatile, light-sensitive [26], can produce off-flavors, modify the color of food products [27], interfere with food texture, and have lower bioavailability [28]. In this sense, some nanotechnological approaches are currently applied to overcome these disadvantages without affecting the therapeutic properties. The nano-encapsulation of EOs by different nanocarriers (Figure 1), mainly those with affinity for hydrophobic molecules such as liposomes, nanoemulsions, solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC), polymer nanocapsules, cyclodextrins, and chitosan-based system (CHT), among others, is a promising alternative for advances in the preservation and microbial control (Table 1) of food products [29–31]. Moreover, the different nanocarriers designed for food applications have to be biocompatible, biodegradable, scaled up, and classified as GRAS (generally recognized as safe) [28], which requires technical evidence of safety [32].

This paper provides an overview of EO-related nanosystems with application for different food matrices. The selection criteria of data were focused on the robust works that provided structural characterization of nanoparticles, physicochemical stability, and efficacy assays against food-related pathogens. The resulting data compilation is available in Table 1, containing the composition, types of bioactive compounds, and bactericidal activity against different strains of interest in the FI, as well as the structural properties of the developed nanosystems.

Essential Oil-Based Nanoparticles as Antimicrobial Agents in the Food Industry - Image 1

Figure 1. Illustrative chart regarding the use and advantages of different organic nanoparticles loading essential oils (EOs) throughout the food production process. EO: essential oil; SLN: solid lipid nanoparticle; NLC: nanostructured lipid carriers.

2. Production Chain in the Food Industry (FI)

All steps of the food production and processing system (farm to fork) are prone to microbial contamination, with direct consequences to the final consumer, causing different foodborne diseases. Therefore, FIs have a huge challenge in searching new strategies for pathogen control. The misuse of antimicrobials has resulted in a selection of bacteria that are more tolerant and resistant to chemical agents, causing risks to human health, due to ineffective therapeutic efforts [33]. In addition, consumers are concerned about the adverse effects of synthetic components used in food [34]. Regulatory issues related to banning the use of these compounds in animal production [35] are in line with the trend towards consuming foods treated with safe and natural antimicrobials [36].

Antimicrobial resistance has been known since the discovery of antibiotics. The continuous and rapid evolution of microorganisms overcome the effect of antimicrobial agents. Therefore, the drug resistance is a major concern, as human and animal health, food production, and environment are interconnected [37]. Compared to traditional antimicrobial drugs, EO can reach different microbial targets [38], such as binding bacterial proteins and lowering pH [39] that improve the therapeutic effect against multidrug-resistant bacteria present in food [40], such as Klebsiella pneumoniae, Campylobacter jejuni, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella spp., Shigella sp., Enterococcus sp., and Escherichia coli [41], and can be used from animal production to the finished product.

Food-grade nanoparticulate products are already available on the market and include nano-additives and nano-ingredients made from-3 fatty acids, incorporated into yo gurts [42] and fruit juices [43]. They have the function of not only enriching the product, but also promoting the sensory acceptability of fish oil [44]. Other products developed by Aquanova® include nanoemulsions loading vitamins. Other nanoencapsulated vitamins (Lypo-Spheric Vitamin, Livon Laboratories, Inc. Company Profile, Henderson, NV, USA) manufactured by LivOn Labs offer protection against their degradation in the gastrointestinal system, increasing the bioavailability [45]. There are also some reports related to the NP application for sensory enhancements, to inhibit flavor loss (infused pure cocoa nanoclusters, RBC Life Sciences ® Inc, Irving, TX, USA) [46], incorporate natural colorants (-carotene, curcumin; NutraLease ®, Ma’ale Adumim, Israel) [47], and offer nanotechno logical advantages, such as Nestlé® patented water-in-oil emulsion (10–500 nm), to facilitate microwave thawing of frozen products [48]. The possibilities of applications are wide, including expressive fat reduction (from 16% to 1%) in ice cream production (Unilever ®, London, UK) [49] and incorporation of antimicrobial compounds [49] in packaging, and in decontamination of industrial equipment [47], demonstrating the diversity of application in food matrices and a dynamic approach to food and feed quality assurance [33].

Table 1.The main properties of nanostructured delivery systems (NDSs loading different essential oils and their uses in the food industry.

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Essential Oil-Based Nanoparticles as Antimicrobial Agents in the Food Industry - Image 3

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Essential Oil-Based Nanoparticles as Antimicrobial Agents in the Food Industry - Image 5

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2.1. Essential Oils with Antimicrobial Activity in Edible Products

Lately, “green nanotechnology” has gained attention, being a recurrent demand of industry and consumers. Minerals, vitamins, antioxidants, natural dyes, micronutrients, and EOscan be exploited using these systems. The uses of EOs as natural antimicrobials and preservatives in FI is growing [37], also aiming at controlling biofilm formation [77,78] without affecting the intrinsic sensory properties of edible products [79]. Several EOs metabolites possess antimicrobial activity, differing in their composition, location, and mechanismsofaction [80]. They can be loaded by different nanoparticles, mainly composed of lipids and/or polymers, and prepared by different methods (supercritical fluid emulsion extraction, spray-dryer, homogenization, emulsification–solvent evaporation, and ultrasonication) [81–84].

In general, it is formed an interface between the EO-based nanosystems and the hydrophobic food matrix, resulting in a synergistic complex, increasing the stability and sustaining the EO release specifically to the target site [3]. In this sense, the bacteria cytoplasmic membrane remodeling is among the most sensitive of cell stabilization in response to biochemical events. Such interactions can promote stretching and compression forces that generate important modifications and deconfiguration in their structure, directly related to the shape of the NP and that allows a higher affinity of interaction with the membrane, causing diffuse elongation and its rupture, due to a mechanical process, induced by tension [85]. As a result, an exacerbated permeability process can occur [86] that affects cellular metabolism and ion leakage, destroys genetic material, and inhibits bacterial quorum sensing signaling [87], also promoting the release of microbial cellular contents, leading to the death of the pathogen [88]. Preventive mechanisms against reactive oxygen species, protein oxidation, and mitochondrial alterations complement the cell damage, promoting injury and interruption of microbial development.

It is important to note that other mechanisms of action include access to the periplasm[89], adenosine triphosphate ATP alteration and cellular hyperpolarization [90], modification of the melting point of membrane microenvironment [91], reduction of the membrane protein interaction, interference in flagella formation, and microbial movement [92] and alteration of the fatty acid organization of the membrane [93]. The barrier imposed by the type of cell membrane, especially in Gram-negative species, is almost completely impermeable to the hydrophobic molecules. More studies are still required of the unique components of these oils (location of hydroxyl groups, alkyl groups, type of aromatic rings) in multiple strains of microorganisms, even belonging to the same family. Thus, the EO therapeutic potential is determined by the spectrum of microorganisms it can inhibit [5]. This includes evaluating whether the action of the active compounds is the same [94], which allows incipient promotion, in consideration of a standardized tetrad of action: nano–EO–bacteria–food matrix. Furthermore, EO can also act as a powerful tool to reduce bacterial resistance [95], evidenced by increased research in various food fields, such as f ish [96], chicken pâté [56], milk [79], pork [51], and beef [97], revealing the urgent demand for innovation in the field of food safety. In order to make the use of EOs viable at a large scale, it is necessary to include data such as how much demand there will be for the product; whether this will be seasonal or not; check where, exactly, the customers are; what the market outlook is; check the existence and availability of resources; define what taxes will have to be paid; and who will work in the production and management process.

2.2. The Drawbacks of Industrial Use of Essential Oils (EOs)

There are relevant EOs limitations that prevent their large use in functional food developments, such as the strong aroma, instability, volatile compounds, sensitivity to processing conditions [98], and controlling to avoid degradation during the processing and consumption stages. Another problem is its storage, which should be kept in closed containers, away from the light and extreme temperatures to avoid oxidation, peroxidation (essential oils—ECHA) [99], and polymorphic rearrangement process [100]. Their effectiveness varies depending on the form of application, the concentration, and storage conditions [79].

Although there are criticisms of the addition of EO in dairy products, reports from the FI attributed several benefits, such as their incorporation as flavoring agents, improving functional properties of chocolate-based products with 0.1% cinnamon EO. However, the use of higher EO concentration (0.3% and 0.5%) will show limited acceptability [101]. This also occurs in food packaging, as the EO strong taste is a limiting factor that can alter the original taste of the food. Encapsulation is possible in order to overcome this adverse condition and widen the variety of food product applications [102]. The application of NPs for food delivery requires that this system should be economically feasible for scale-up of production [103,104], and offers specifics advantages, including improved food matrix compatibility, low-cost storage and simple use, delayed chemical degradation, prolonged release, softening of undesirable off-flavors, and increased antimicrobial potency [105].

3. Nanostructured Delivery Systems (NDSs)

The nanostructured delivery systems (NDSs) are molecularly composed of different biomaterials and are processed as several forms in order to interact specifically with the targets.

There are a lot of benefits of the EOs nanoencapsulation approach, such as bioactive protection to the external environment (e.g., products rich in unsaturated lipids can be peroxided over time); sustained release; desirable shelf time; to mask eventual bioactive unpleasant odor and/or flavor; and act as smart packaging with moisture maintenance, protection against pathogens, and monitoring of conditions during distribution [72,81,106]. Different NPs loading EOs have been proposed for pathogen control or incorporated as adjuvants in food-based products [41]. Furthermore, nanoencapsulation presents itself as a method to protect against formation of degraded compounds with toxic derivatives, such as the conversion of safrole (4-allyl-1,2-methylene dioxybenzene), derived from plants of the Lauraceae family (including nutmeg and black pepper) with hepatocarcinogenic effects [81,107].

Thus, the intended application of EO-loaded nanocarriers will direct the selection of the more appropriate nanosystems, excipients, and concentrations, followed by the most viable preparation method. This early planning is essential to ensure a successful preparation, long-term stability, and high rates of encapsulation efficiency of a bioactive [93]. Therefore, the most developed NDS loading EOs for FI uses are discussed in Table 1 below, with key biological results.

3.1. Liposomes

Liposomes are spherical vesicles composed of phospholipids spontaneously oriented as lipid bilayers (unilamellar or multilamellar) in aqueous solutions, given by the hydrophobic interactions of nonpolar acyl chains [108]. The amphiphilic nature of lipids is currently exploited to encapsulate food ingredients of different polarities, creating an efficient physical barrier system and protecting bioactives from environmental conditions [109]. The lipid bilayers allow interaction with the hydrophobic encapsulated compounds, being intrinsically related to NDS stability (fluidity, permeability, and polarity, among others), as well as encapsulation efficiency and sustained release profile [110].

In general, the analyzed reports showed that the EO-loaded liposomes provided optimized antimicrobial activity. Sebaaly et al. (2021) developed multilamellar liposomes composed of dipalmitoyl phosphatidylcholine (DPPC) encapsulating EO from Z. tingoassuiba, with a mean diameter of 9.37 4.69 m [111]. The in vitro antimicrobial activity was evaluated by the disc diffusion test. Antimicrobial activity against Staphylococcus aureus, Micrococcus luteus, and Streptococcus mutans, important pathogens related to the food infections, was observed [110].

Another possible application of EO-loaded liposomes for antimicrobial control is incorporation into food packaging, as recently described [51]. In this work, laurel EO was encapsulated by silver nanoparticles and liposomes. The samples were incorporated in pork packaging as preservatives. Liposomes with an average size of 200 nm and a sustained release profile of laurel EO were obtained for both nanosystems, with values around of 29.30% and 11.79% for laurel-EO-loaded liposomes and laurel-based silver nanoparticles, respectively, after 7 days and stored at room temperature. In addition, they presented satisfactory antimicrobial and antioxidant activity, increasing the storage of animal protein to 15 days when kept at 4 C[51].

Liposomes offer unique function in the transport of hydrophobic and hydrophilic molecules, allowing its applications in different food matrices (dairy, meat, beverages, and confectionery). Furthermore, the encapsulation of two or more EOs in the same systems can be exploited to achieve beneficial synergistic effects.

3.2. Nanoemulsions (NEs)

Nanoemulsions (NEs) are stable systems composed of two immiscible phases [112], being processed as water-in-oil or oil-in-water, stabilized by amphiphilic surfactant and co-surfactant. They exhibit excellent structural properties, with particle size between 50–250 nm and a monodisperse distribution [113]. Food-grade NEs containing bioactive components can be used to develop biodegradable packaging films. Promising results were provided of NEs that prevented foodborne diseases. In this sense, carvacrol-based NE was tested in minimal processes as an alternative to calcium hypochlorite use against Escherichia coli O157:H7 and Salmonella Typhimurium in contaminated broccoli and radish seeds. A system containing 0.4% carvacrol was able to inactivate contamination in low levels (about 2–3 log CFU/g) in radish seeds. The treatment was not effective for broccoli seeds [52].

NEscan also be applied in milk quality control. Jemaa et al. (2018) noted the efficiency of Thymus capitatus essential oil in controlling S. aureus, which resulted in colony count decreases of 202 103 CFU/mLwith the pure oil and 132 103 CFU/mLwhentreated with NEs after 24 h of bacterial inoculation [114]. In both treatments, increase in the inhibition of bacterial growth was observed. Such systems can also be used in animal products without changing the physicochemical characteristics of the product, such as chicken pâté, with the incorporation of NE with different amounts of oregano EO(Origanum vulgare) [56].

NEs have huge potential for application in FI. Several NE-based products are already commercially available. However, there are still some concerns related to the economic viability of large-scale production, due to the relatively expensive production costs.

3.3. Solid Lipid Nanoparticles (SLNs)

SLNs emerged in 1991, firstly described by Professors Müller and Gasco, as an alternative to traditional colloidal systems such as liposomes [115,116]. This system is based on a lipid matrix composed of solid lipid stabilized by a surfactant [30,116]. SLNs have high affinity to load hydrophobic active into the solid matrix [117]. SLNs allow to improve the stability of loaded EOs under various adverse conditions, including food processing, heating, high pressure, drying, UV radiation, and bile salt [118]. Therefore, such a system was designed for applications in food distribution because it has interesting advantages to be used in this sector, such as the effective uptake of fat-soluble assets, sustained release profile, and long-term stability [119,120].

In meat products, SLN loading curcumin was evaluated in the antimicrobial activity assay of hamburger patties inoculated with foodborne pathogens. It was shown that curcumin-based SLN was more effective against Gram-positive than Gram-negative bacteria, stored for 8 days [121]. However, this optimized effect against Gram-positive species is not a rule, since parameters of different lipid compositions of SLN and the cell wall of microorganisms are primary factors [122] that modulate the biological activities of NDS. The activity against Gram-negative bacteria such as Salmonella typhi was also demonstrated. SSLNs containing Eugenia caryophyllata EO were noted and less effective for Gram-positive bacteria (S. aureus), and more effective for Gram-negative bacteria and fungi, in microbial killing [83].

There are a lot of preparation methods and lipid excipients that act as structural bioactive matrices of SLNs, being reproductible at a large scale. Such systems present an emerging field to encapsulate EOs with bactericidal activity [123]. However, in order to allow SLNs application in the FI, more information is still needed regarding their compatibility with the food interface without negatively affecting the physicochemical and organoleptically properties of food products.

3.4. Nanostructured Lipid Carriers (NLCs)

NLCs are the second generation of lipid nanoparticles, composed of a lipid matrix formed by a blend between solid and liquid lipids in room temperature, stabilized by a surfactant [115]. They are also considered to be safe and promising nanocarriers for the delivery of hydrophobic compounds, despite their hydrophilicity (colloid liquid systems), which is paramount for the establishment of water-based foods. Therefore, NLCs have industrial relevance as these nanocarriers also combine specific advantages for food uses, such as the ability to be successfully incorporated into transparent and opaque foods and beverages [115]. The encapsulation of EOs by NLCs has been proposed to exhibit higher stability, minimized toxicity, and preserve the therapeutic properties of pristine EOs [124]. EO-loaded NLC formulations have been widely described to increase the antimicrobial activity of EOs and protect them against environmental damage conditions (light, hydrolysis, and evaporation, among others) [31,125].

It is hypothesized that the mechanisms of action of NLCs against bacterial species refer to the nanostructured particles’ sizes together with their surface charges, culminating in microorganism deaths. Cationic NLCs, when in contact with Gram-negative bacteria, will be electrostatically attracted by the membrane, increasing the EO release at the specific target and causing enzymatic reactions that lead to its rupture [126]. In the case of anionic NLCsin contact with Gram-negative bacteria, the hydrogen bonds between the NLC and bacteria membrane will govern their interactions [127].

Keivani Nahr et al. [61] described a versatile strategy to overcome the limitation of applying cardamom EO for food uses. In this sense, cardamom EO was loaded by NLC (with cocoa butter as solid lipid) and applied to water-based foods. Cardamom EO is used to provide flavor to a wide range of processed foods, and it was noticed that such a compound presented in vitro antimicrobial properties against S. aureus and E. coli, Gram positive and Gram-negative bacteria, respectively. The average nanoparticle size of NLC was in the range of 118.7–141.7 nm with a polydisperse size distribution (PDI = 0.271–0.468), with excellent negative zeta potential values in the range from 30 to 58 mV [61].

Ribeiro et al. [31] prepared different NLC formulations encapsulating some vegetable and EOsas active molecules against Campylobacter jejuni (CJ), in both free and sessile forms, with high interest in public health and food. The authors provided NLC formulations with particle size lower than 250 nm, monodisperse distribution (PDI < 0.170), and shelf life of over a year when stored at 25 C. Findings were a composition based on ucuuba butter and olibanum EO that presented in vitro minimum inhibitory concentration (MIC) values around 2.60–1.56 mg/mL and 0.78 mg/mL against free and sessile forms of CJ, respectively. Such nanotechnological approach also contributed to an increase in the stability and hydrophilicity of the EO.

These data are relevant and reflect possible advances in the delivery of NLCs with bactericidal effects in FI, since their efficiency was also evaluated on bacteria in a sessile form and whose elimination in these environments is critical. In addition, the use of bioactive excipients to prepare formulations with adequate physicochemical properties and reduce the limitations of pristine EO uses, allied to the large-scale production ability and low productions costs, make both SLN and NLC systems promising NDSs to reach the market.

3.5. Polymer Nanocapsules (NCs)

NCs are NDSs formed by a shell (polymer wall) and core (oil core) counterparts that have interesting physicochemical properties for application in food matrices, such as those derived from aliphatic polyester (-caprolactone), which are stable in storage and allow a sustained release of loaded actives. Such core–shell structure brings benefits that include the difficulty of contact with external substances and is better suited to lipophilic foods [128].

The encapsulation of EOs by polymer nanocapsules (NC) aims to improve hydrophilicity of insoluble compounds, protection against degradation, prevention of loss of volatile compounds caused by evaporation, and a sustained release. Their antimicrobial activity of NCloading Thymus capitatus EO with high amount of bioactives (43% thymol) was reported [8]. The association of polymers and thymol EO has immediate applicability against important pathogens in the FI (S. aureus, L. monocytogenes, B. cereus, S. typhi, S. dysenteriae, and E. coli).

Different synthetic polymers can also be used together with biopolymers. An NC com posed of Balangu seed gum (0.25%), PVA (poly-vinyl alcohol (1%), and Mentha longifolia EO was prepared and showed promising results. This system was tested for EO release profile in various food conditions and showed immediate release in the first 3 min, followed by a continuous and gradual release up to 180 min [129] in aqueous, acidic, and alcoholic media, according to the European Commission regulation 10/2011 EU (10/2011/EC) requirement (EUR-Lex—32011R0010—EN—EUR-Lex, n.d.) [130]. The studies suggest a greater search for compounds that can be incorporated into NCs to verify the action on bacteria commonly related to food products, exploring their action potential.

3.6. Cyclodextrins (CDs)

Cyclodextrins (CDs) are cyclic oligosaccharides that have glucose units linked by-(1,4)-glycosidic bonds, derived from the enzymatic degradation of starch [110], mainly by B. macerans [34]. They present a cone-shaped morphology with a hydrophobic cavity and 7.9 Å diameter, and are able to form inclusion complexes with low-aqueous-solubility molecules [50].

The inclusion complexes formed by CDs and EOs improve the EO solubility and physicochemical stability, providing a sustained release of these compounds [131], also acting as physical barrier to protect the EO in procedures such as freezing, thawing, and/or microwaving during food processing [34]. In the FI, they are used to improve flavor and sorption of bitter compounds. Recently, CDs have been noticed as constituents of active packaging of edible products [132].

There are several commercially available CD-based products, such as dairy products, food supplements, processed foods, teas, coffees, clarified fruits, sweeteners, honey, and mustard sauce [103]. There are also some reports of industrially and enzymatically obtained CDs with modified starch glucosyltransferase, resulting in a nontoxic product fully processed by the microbiota of the gastrointestinal tract [133]. CD applicability also extends to antimicrobial sachets as food preservatives. Pimenta dioica EO, derived from the Myrtaceae family, was effective against S. aureus and food spoilage by B. nivea mycelia. These sachets, composed of cellulose fiber, allowed the diffusion of the volatile compounds complexed with-CD and proved to be effective in the control of food pathogens [134].

Freudenberg et al. were pioneers in the preparation of hybrid systems based on CDs linked to chitosan, aiming to reduce the unpleasant taste and odor of fish oil and vegetable oils. There is still need for more efforts to elucidate a wide range of EOs that can form CD inclusion complexes, since their use in active packaging is already studied and applied in the FI, in order to increase the applications ranges [135].

3.7. Chitosan-Based Delivery Systems (CHT)

Chitosan (CHT) is a linear cationic polysaccharide formed by (1,4)-amino-deoxy b-d-glucan chains derived from a deacetylated chitin of crabs and crustaceans, and is biocompatible, biodegradable, and cheap [64]. The use of CHT as a drug carrier has additional advantages as an NDS, such as the protection of the selected active, changes in temperature, and sustained release at the site of interest [136], given by the electrostatic interactions with anionic biological membranes. As a biopolymer, it can be processed as several forms and is abundant, cheap, and biodegradable [137].

CHT application in association with EOs has demonstrated higher antimicrobial activity against foodborne pathogens than biopolymer films and coatings [64]. Specifically, it has demonstrated its advantageous use as an NDS in food preservatives, as it allows a sustained release of active ingredients in the production of food films and coatings [64]. Its inherent antimicrobial activity has been attributed to the electrostatic forces between the cationic amino groups (NH3) of CHT and the negatively charged residues of the fatty acids located on the bacterial [138].

CHTprocessed as films has been shown to delay the release of EOs, also with high concentration of EOs, evaluated in packaging systems [139] for preservation of poultry meat [26], ground beef [140], fresh shrimp [62], and beef [97]. A recent work shows that CHTfilms functionalized with zein nanoparticles (ZNs) loading EO of Cinnamodendron dinisii Schwanke produced stable, homogeneous ZNs with zeta potential values around +30 mV and a polydispersity index less than 0.2, with size in the range of 70–110 nm. CHTNPsloading Thymus capitatus EO demonstrated an encapsulation efficiency around 68%, a maximum release profile after 360 min and optimized antimicrobial activity [141]. Other reports described the antimicrobial activity of CHT film through the disc diffusion test. It was demonstrated that the CHT film loading zein-EO nanoparticles increased the antimicrobial activity against Salmonella typhimurium and Shigella flexneri [142]. In fish, different EO incorporated into gelatin–CHT films were evaluated against different food pathogens and bacteria that lead to decay. Clove-EO-loaded CHT film showed desirable inhibitory action against six targeted microorganisms, including L. innocua and E. coli [96]. A novel proposed development of two-layer films composed of CHT–PVA carrying Zataria multiflora and cinnamon EO was investigated as an active food packaging effective against S. aureus and Aspergillus flavus. These results were attributed to the increased hydrophilic property of PVA, aiding in the desired release of antimicrobial agents [140].

The nanocarriers mentioned above can also be combined with other natural biomaterial, such as the association of liposomes and chitosan that results in chitosomes hybrid formulations that contribute to liposomal stability. The supramolecular organization of such a hybrid system is provided by the liposome surface functionalization, through the formation of a CHT-coating, which can also ensure a targeted delivery and increased antimicrobial efficacy of the EO [111]. The development of a CHT membrane containing tea tree EOloaded by liposomes was reported to have an observed rate of inhibition of Salmonella sp. around 99.9%, in an assay with raw chicken packages, after 4 days of treatment, without considerable impact on sensory quality [63]. These data are comparable to irradiation and acidified sodium chloride as chemical treatments of chicken carcasses for reducing the risk of Salmonella in chicken meat processing [143]. Additionally, CHT-based membrane loading Eos in liposomes presents low toxicity and cost.

There are still some factors to be elucidated to expand CHT uses, mainly because its allergen ability, low hydrophilicity, and organoleptic characteristics (fish-like odor and taste) depend on the concentration used. In general, NDS-based CHT seems to be a biocompatible product when combined with other active principles, in addition to its intrinsic antimicrobial capacity.

4. Nanopackaging and Smart Control of Food Pathogens

EOs are widely explored for use in food packaging due to their potential antimicrobial activity [98]. Antimicrobial packaging is also able to maintain both the nutritional and sensory quality of food, extending its shelf life. Such approach seems to be an effective alternative to the addition of synthetic preservatives in the packages, as it will be in contact with the food products. Therefore, EOs are currently incorporated into the packages, aiming to decrease the maximum population growth rate and/or prolong the lag phase of the target microorganism by contact inactivation. When used in adequate concentrations, in addition to inhibiting foodborne pathogenic bacteria (Listeria, Salmonella, Aeromonas, Clostridium botulinum, Enterobacter, and Staphylococci) and their toxins, EOs can also improve the sensory properties of the food products [144]. However, the EOs incorporation in active and smart packaging needs to meet crucial safety requirements. For instance, the slow denaturation of EOs from the packaging material to the food surface allows maintaining a high concentration of EOs over a long period of time when compared to the direct addition of EOs in food, the result of which differs by the rapid diffusion of the bioactive with a consequent reduction in antimicrobial activity [145].

The application of nanotechnology in packaging development presents interesting opportunities to exploit the antimicrobial properties of EOs [146]. This strategy has been widely explored, mainly because they act as a physical barrier leading to decreased spoilage and improved inhibition of pathogenic microorganism growth [147]. Nanofilms composed of a thin layer of polymers, created using layer-by-layer deposition techniques, have potential applications in the field of active food packaging. They have been applied directly on the product, whereas non-edible coatings have a protective function specifically on the packaging. Their applicability also extends to surfaces that are in contact with food during processing or to prevent the formation of biofilms [28].

Final Product: Pure or Nanoencapsulated Essential Oils in Dairy and Meat Products

A supply chain challenge is ensuring that the final product has a distribution of predefined and quality standards, particularly considering the globalization of the food trade and the many current centralized processing facilities that manage a standardized food distribution [148]. The EOs are considered GRAS by the U.S. Food and Drug Administration (FDA) [29,149,150], and are widely used for several purposes, including maintaining safety and improving factors such as microbial reduction and sensory characteristics when applied to meat, chicken, milk, and fruit juice products [56,79,81,151,152].

EOs have different bioactive components, with effects that can be additive or synergistic [153], useful for improving the sensory and nutritional properties of dairy products [27]. Table 1 summarizes different NDS properties with huge interests in the FI, demonstrating their ability to control or eliminate pathogens. The application of nanosystems encapsulating EOs offers several advantages, ensuring their use as promising nanostructured formulations for food application. A synergy between the mixtures of EOs in animal products is observed [79]. Such an approach has shown to be one of the best natural alternatives to pasteurization, to prevent food spoilage by microbes in various food matrices [154], and can be an effective alternative in microbiological control in countries where raw milk consumption is allowed [155,156].

However, it is important to ponder the use of higher concentrations of EOs, due to their sensory effects and the interactions with the physical components of dairy products. The interaction of EO phenolic compounds occurs from the formation of a layer on the surface of some hydrophobic components of proteins and fats in dairy products, which restricts the EO bioavailability. Xue and colleagues noted that the level of fat in dairy products also influenced the inhibition of L. monocytogenes. The antimicrobial activity of NE in low-fat milk (5 log CFU/mL) was higher for whole milk (3 log CFU/mL) after 48 h of storage [157]. The effect caused can be explained by the greater action of hydrophobic antimicrobial agents in whole milk, which has higher amounts of fat [158]. The mentioned approaches of incorporating EOs into films and edible coatings, as well as their nanoencapsulation by different nanocarriers, contribute to maintaining EOs’ therapeutic action [158]. These systems minimize the effects of interactions with the food matrix (fat, carbohydrates, or proteins) [27,159–161]. Several works have evaluated the addition of EOs to meat and milk products and their derivatives. Other reports evaluated EOs with antimicrobial effect against pathogens of ultra-filtrated soft cheese [162] and ice cream [163]. A decrease in total mesophilic and psychrophilic aerobic bacteria was evaluated. Similarly, NE-based edible antimicrobial coatings prepared from oregano EO extended the shelf life of low-fat cheese [164]. Mint, fennel, and basil EO in yoghurt were able to inhibit the microbial growth [165]. Meat and related products are more prone to spoilage due to the high levels of protein, high water activity, and diverse microorganisms [121]. Pathogenic bacteria in meat are responsible for a significant number of foodborne outbreaks (Listeria monocytogenes, Salmonella spp., E. coli O157:H, and S. aureus) and deaths [166].

Several works have reported the promising effects of EOs when incorporated in meat products to control these problems. Pathogen control of meat has also been demonstrated with geraniol- and linalool-EO-loaded NEs. Growth inhibition of E. coli K12, Listeria in nocua, and Pseudomonas lundensis in a simulated meat system was observed [167]. Other work described a control of S. aureus and Enterobacteriaceae, using chitosan/cinnamon EO-loaded NE analyzed in meat patties [151]. The application of EO encapsulated by nanosystems should offer a set of advantages that ensures its use as promising nanostructured formulations in FI application. The use of EOs in the food chain is beneficial to inhibit microorganisms contaminations, which justifies its use. It is still necessary to spend more effort to expand the analyses of other EOs loaded by nanosystems still unknown, with novel properties to be explored, increasing the range of alternatives to synthetic preservatives in the FI.

5. Conclusions

Alternative control antimicrobials are provided to reduce the occasional food risk from pathogens, increasing the quality and shelf life of products. Specific in vitro and in vitro toxicity tests for application in the FI need to be standardized in order to ensure the safety of these new compounds.

Specifically, regarding the regulatory issues, there are some safety indications on nanoscience and nanotechnology established by the scientific committee of the European Food Safety Agency (2009) for humans [1] (EFSA, 2020 [168]), and in 2011, a guidance of the use of nanomaterials in animal feed by the FDA (Government of the United States, n.d.). According to Brazil, a robust international regulatory framework for the evaluation of nanotechnology-based products is necessary for approval of food and feed containing nanoparticles. Despite the advances already reported, there is no standard protocol for the toxicity testing of nanomaterials that assesses, for instance, the NP excretion mechanisms after digestion [169]. Specifically regarding NDSs for FI applications, it is indispensable to provide safe, stable, and efficient products. It was noted that the complexity of the food matrix (food matrix pH, temperature, and storage time) makes it difficult to investigate NP migration and toxicity. There are still no global regulations for manufacturing, labeling, or in vivo and in vitro toxicity testing, nor for potential environmental harm from nanoparticle residues [170,171].

We emphasized the use of NLCs as a promising alternative when analyzing the aspects of microbiological control and compatibility with foods. It can be prepared with different mixtures between liquid and solid lipids, molecularly planned according to the target bacterium, being resistant to pH variations, food conservation, and stored at room temperature, highlighting desirable aspects for the production of an effective compound that can be produced on a large scale, for use in industries.

The assessment of risk factors is certainly a concern for the subsequent approval of food-based nanostructured products. It is expected that in the near future, FIs will incorporate nanotechnological tools in their production processes, contributing to the effective microbiological control, according to the standards required for each type of food produced. It can increase the specificity of products against target microorganisms for each food.

This article was originally published in Microorganisms 2022, 10, 1504. https://doi.org/10.3390/ microorganisms10081504. This is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

References

1. Menossi, M.; Ollier, R.P.; A Casalongué, C.; Alvarez, A.V. Essential oil-loaded bio-nanomaterials for sustainable agricultural applications. J. Chem. Technol. Biotechnol. 2021, 96, 2019–2122. [CrossRef]

2. Román, S.; Sánchez-Siles, L.M.; Siegrist, M. The importance of food naturalness for consumers: Results of a systematic review. Trends Food Sci. Technol. 2017, 67, 44–57. [CrossRef]

3. Preethi, R.; Dutta, S.; Moses, J.A.; Anandharamakrishnan, C. Green nanomaterials and nanotechnology for the food industry. In Green Functionalized Nanomaterials for Environmental Applications; Elsevier: Amsterdam, The Netherlands, 2022; pp. 215–256.

4. Ezhilarasi, P.N.; Karthik, P.; Chhanwal, N.; Anandharamakrishnan, C. Nanoencapsulation Techniques for Food Bioactive Components: A Review. Food Bioprocess Technol. 2013, 6, 628–647. [CrossRef]

5. Nair, A.; Mallya, R.; Suvarna, V.; Khan, T.A.; Momin, M.; Omri, A. Nanoparticles—Attractive Carriers of Antimicrobial Essential Oils. Antibiotics 2022, 11, 108. [CrossRef]

6. Christaki, E.; Bonos, E.; Giannenas, I.; Florou-Paneri, P. Aromatic Plants as a Source of Bioactive Compounds. Agriculture 2012, 2, 228–243. [CrossRef]

7. Shatalov, D.O.; Kedik, S.A.; Zhavoronok, E.S.; Aydakova, A.V.; Ivanov, I.S.; Evseeva, A.S.; Beliakov, S.V.; Biryulin, S.I.; Kovalenko, A.V.; Mikhailenko, E.N. The current state and development of perspectives of application of synthetic antimicrobial agents. Polym. Sci. Ser. D 2017, 10, 293–299. [CrossRef]

8. Granata, G.; Stracquadanio, S.; Leonardi, M.; Napoli, E.; Consoli, G.M.L.; Cafiso, V.; Stefani, S.; Geraci, C. Essential oils encapsulated in polymer-based nanocapsules as potential candidates for application in food preservation. Food Chem. 2018, 269, 286–292. [CrossRef]

9. Medalla, F.; Gu, W.; Friedman, C.R.; Judd, M.; Folster, J.; Griffin, P.M.; Hoekstra, R.M. Increased Incidence of Antimicrobial Resistant Nontyphoidal Salmonella Infections, United States, 2004–2016. Emerg. Infect. Dis. 2021, 27, 1662–1672. [CrossRef]

10. Hashiguchi, T.C.O.; Ouakrim, D.A.; Padget, M.; Cassini, A.; Cecchini, M. Resistance proportions for eight priority antibiotic bacterium combinations in OECD, EU/EEA and G20 countries 2000 to 2030: A modelling study. Eurosurveillance 2019, 24, 1800445. [CrossRef]

11. Filipiak, W.; Mochalski, P.; Filipiak, A.; Ager, C.; Cumeras, R.; Davis, C.E.; Agapiou, A.; Unterkofler, K.; Troppmair, J. A Compendium of Volatile Organic Compounds (VOCs) Released by Human Cell Lines. Curr. Med. Chem. 2016, 23, 2112–2131.[CrossRef]

12. Capelezzo, A.P.; Mohr, L.C.; Dalcanton, F.; de Mello, J.M.M.; Fiori, M.A.-Cyclodextrins as Encapsulating Agents of Essential Oils. In Cyclodextrin. A Versatile Ingredient; Poonam, A., Neelima, D., Eds.; IntechOpen: London, UK, 2018. [CrossRef]

13. Dudareva, N.; Klempien, A.; Muhlemann, J.K.; Kaplan, I. Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytol. 2013, 198, 16–32. [CrossRef]

14. Thormar, H. Lipids and Essential Oils as Antimicrobial Agents; Thormar, H., Ed.; John and Wiley and Sons: Hoboken, NJ, USA, 2011; ISBN 9780470741788.

15. Leitão, S.G.; De Oliveira, D.R.; Sülsen, V.; Martino, V.; Barbosa, Y.G.; Bizzo, H.R.; Lopes, D.; Viccini, L.F.; Salimena, F.R.G.; Peixoto, P.H.P.; et al. Analysis of the chemical composition of the essential oils extracted from Lippia lacunosa Mart. Schauer and Lippia rotundifolia Cham. (Verbenaceae) by gas chromatography and gas chromatography-mass spectrometry. J. Braz. Chem. Soc. 2008, 19, 1388–1393. [CrossRef]

16. Yan, D.; Wong, Y.F.; Tedone, L.; Shellie, R.; Marriott, P.J.; Whittock, S.; Koutoulis, A. Chemotyping of new hop (Humulus lupulus L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry. J. Chromatogr. A 2018, 1536, 110–121. [CrossRef]

17. Bilia, A.R.; Guccione, C.; Isacchi, B.; Righeschi, C.; Firenzuoli, F.; Bergonzi, M.C. Essential Oils Loaded in Nanosystems: A Developing Strategy for a Successful Therapeutic Approach. Evid. Based Complement. Altern. Med. 2014, 2014, 651593. [CrossRef]

18. Garcia, A.; Barbas, C. Gas Chromatography-Mass Spectrometry (GC-MS)-Based Metabolomics. In Metabolic Profiling; Humana Press: Totowa, NJ, USA, 2010; pp. 191–204. [CrossRef]

19. Satyal, P.; Jones, T.H.; Lopez, E.M.; McFeeters, R.L.; Ali, N.A.A.; Mansi, I.; Al-Kaf, A.G.; Setzer, W.N. Chemotypic Characterization and Biological Activity of Rosmarinus officinalis. Foods 2017, 6, 20. [CrossRef]

20. Enioutina, E.Y.; Teng, L.; Fateeva, T.V.; Brown, J.C.S.; Job, K.M.; Bortnikova, V.V.; Krepkova, L.V.; Gubarev, M.I.; Sherwin, C.M.T. Phytotherapy as an alternative to conventional antimicrobials: Combating microbial resistance. Expert Rev. Clin. Pharmacol. 2017, 10, 1203–1214. [CrossRef]

21. Sharifi-Rad, M.; Varoni, E.M.; Salehi, B.; Sharifi-Rad, J.; Matthews, K.R.; Ayatollahi, S.A.; Kobarfard, F.; Ibrahim, S.A.; Mnayer, D.; Zakaria, Z.A.; et al. Plants of the Genus Zingiber as a Source of Bioactive Phytochemicals: From Tradition to Pharmacy. Molecules 2017, 22, 2145. [CrossRef]

22. Tajkarimi, M.M.; Ibrahim, S.A.; Cliver, D.O. Antimicrobial herb and spice compounds in food. Food Control. 2010, 21, 1199–1218. [CrossRef]

23. Saeed, F.; Afzaal, M.; Tufail, T.; Ahmad, A. Use of Natural Antimicrobial Agents: A Safe Preservation Approach. In Active Antimicrobial Food Packaging; IntechOpen: London, UK, 2019. [CrossRef]

24. Karásková, K.; Suchý, P.; Straková, E. Current use of phytogenic feed additives in animal nutrition: A review. Czech J. Anim. Sci. 2016, 60, 521–530. [CrossRef]

25. Kholif, A.E.; Anele, U.Y.; Patra, A.K.; Varadyova, Z. Editorial: The Use of Phytogenic Feed Additives to Enhance Productivity and Health in Ruminants. Front. Vet. Sci. 2021, 8, 685262. [CrossRef]

26. Zhang, X.; Ismail, B.B.; Cheng, H.; Jin, T.Z.; Qian, M.; Arabi, S.A.; Liu, D.; Guo, M. Emerging chitosan-essential oil films and coatings for food preservation—A review of advances and applications. Carbohydr. Polym. 2021, 273, 118616. [CrossRef]

27. Chivandi, E.; Dangarembizi, R.; Nyakudya, T.T.; Erlwanger, K.H. Use of Essential Oils as a Preservative of Meat. In Essential Oils in Food Preservation, Flavor and Safety; Academic Press: Cambridge, MA, USA, 2016; pp. 85–91. [CrossRef]

28. Joye, I.J.; Davidov-Pardo, G.; McClements, D.J. Nanotechnology in Food Processing. In Encyclopedia of Food and Health; Elsevier: Amsterdam, The Netherlands, 2016; pp. 49–55.

29. Kumar,A.; Singh, P.; Gupta, V.; Prakash, B. Application of nanotechnology to boost the functional and preservative properties of essential oils. In Functional and Preservative Properties of Phytochemicals; Elsevier Inc.: Amsterdam, The Netherlands, 2020; pp. 241–267; ISBN 9780128185933. [CrossRef]

30. Prakash, B.; Kujur, A.; Yadav, A.; Kumar, A.; Singh, P.P.; Dubey, N.K. Nanoencapsulation: An efficient technology to boost the antimicrobial potential of plant essential oils in food system. Food Control 2018, 89, 1–11. [CrossRef]

31. Ribeiro, L.N.D.M.; de Paula, E.; Rossi, D.A.; Martins, F.A.; de Melo, R.T.; Monteiro, G.P.; Breitkreitz, M.C.; Goulart, L.R.; Fonseca, B.B. Nanocarriers from Natural Lipids With In Vitro Activity Against Campylobacter jejuni. Front. Cell. Infect. Microbiol. 2021, 10, 571040. [CrossRef]

32. FDA.Guidance for Industry Use of Nanomaterials in Food for Animals; FDA: Silver Spring, MD, USA, 2015; p. 10.

33. Melo, R.T.; Galvão, N.N.; Guidotti-Takeuchi, M.; Peres, P.A.B.M.; Fonseca, B.B.; Profeta, R.; Azevedo, V.A.C.; Monteiro, G.P.; Brenig, B.; Rossi, D.A. Molecular Characterization and Survive Abilities of Salmonella Heidelberg Strains of Poultry Origin in Brazil. Front. Microbiol. 2021, 12, 674147. [CrossRef]

34. Pereira, A.G.; Carpena, M.; Oliveira, P.G.; Mejuto, J.; Prieto, M.; Gandara, J.S. Main Applications of Cyclodextrins in the Food Industry as the Compounds of Choice to Form Host-Guest Complexes. Int. J. Mol. Sci. 2021, 22, 1339. [CrossRef]

35. Wu,Z.Antimicrobial use in food animal production: Situation analysis and contributing factors. Front. Agric. Sci. Eng. 2018, 5, 301. [CrossRef]

36. Quinto, E.J.; Caro, I.; Villalobos-Delgado, L.H.; Mateo, J.; De-Mateo-Silleras, B.; Redondo-Del-Río, M.P. Food Safety through Natural Antimicrobials. Antibiotics 2019, 8, 208. [CrossRef]

37. Iriti, M.; Vitalini, S.; Varoni, E.M. Humans, Animals, Food and Environment: One Health Approach against Global Antimicrobial Resistance. Antibiotics 2020, 9, 346. [CrossRef]

38. Kon,K.V.; Rai, M.K. Plant essential oils and their constituents in coping with multidrug-resistant bacteria. Expert Rev. Anti-Infect.Ther. 2012, 10, 775–790. [CrossRef]

39. Ruddaraju, L.K.; Pammi, S.V.N.; Guntuku, G.S.; Padavala, V.S.; Kolapalli, V.R.M. A review on anti-bacterials to combat resistance: From ancient era of plants and metals to present and future perspectives of green nano technological combinations. Asian J.Pharm. Sci. 2019, 15, 42–59. [CrossRef]

40. Pérez-Rodríguez, F.; Taban, B.M. A State-of-Art Review on Multi-Drug Resistant Pathogens in Foods of Animal Origin: Risk Factors and Mitigation Strategies. Front. Microbiol. 2019, 10, 2091. [CrossRef]

41. Chouhan, S.; Sharma, K.; Guleria, S. Antimicrobial Activity of Some Essential Oils—Present Status and Future Perspectives. Medicines 2017, 4, 58. [CrossRef]

42. Ghorbanzade, T.; Jafari, S.M.; Akhavan, S.; Hadavi, R. Nano-encapsulation of fish oil in nano-liposomes and its application in fortification of yogurt. Food Chem. 2017, 216, 146–152. [CrossRef]

43. Ilyasoglu, H.; El, S.N. Nanoencapsulation of EPA/DHA with sodium caseinate–gum arabic complex and its usage in the enrichment of fruit juice. LWT 2013, 56, 461–468. [CrossRef]

44. McClements, D.J.; Öztürk, B. Utilization of Nanotechnology to Improve the Handling, Storage and Biocompatibility of Bioactive Lipids in Food Applications. Foods 2021, 10, 365. [CrossRef]

45. Dasgupta,N.; Ranjan, S. AnIntroduction to Food Grade Nanoemulsions; Springer: Singapore, 2018; Volume 1, ISBN 978-981-10-6985-7.

46. Ranjan, S.; Dasgupta, N.; Chakraborty, A.R.; Samuel, S.M.; Ramalingam, C.; Shanker, R.; Kumar, A. Nanoscience and nanotech nologies in food industries: Opportunities and research trends. J. Nanoparticle Res. 2014, 16, 2464. [CrossRef]

47. Silva, H.D.; Cerqueira, M.; Vicente, A.A. Nanoemulsions for Food Applications: Development and Characterization. Food Bioprocess Technol. 2012, 5, 854–867. [CrossRef]

48. Kirby, C.J. Nanotechnology in the Food Sector. In Food Processing Handbook, 2nd ed.; Brennan, G.J., Grandison, A.S., Eds.; John and Wiley and Sons: Hoboken, NJ, USA, 2009; Volume 1, pp. 693–726. [CrossRef]

49. Gupta, S.V.S. Nanotechnology and Food Science: Tomorrow Design the Food. Int. J. Curr. Microbiol. Appl. Sci. 2017, 6, 3553–3561. [CrossRef]

50. Detoni, C.B.; Cabral-Albuquerque, E.C.M.; Hohlemweger, S.V.A.; Sampaio, C.; Barros, T.F.; Velozo, E.S. Essential oil from Zanthoxylum tingoassuiba loaded into multilamellar liposomes useful as antimicrobial agents. J. Microencapsul. 2009, 26, 1–8. [CrossRef]

51. Wu,Z.;Zhou, W.; Pang, C.; Deng, W.; Xu, C.; Wang, X. Multifunctional chitosan-based coating with liposomes containing laurel essential oils and nanosilver for pork preservation. Food Chem. 2019, 295, 16–25. [CrossRef]

52. Landry, K.S.; Micheli, S.; McClements, D.J.; McLandsborough, L. Effectiveness of a spontaneous carvacrol nanoemulsion against Salmonella enterica Enteritidis and Escherichia coli O157:H7 on contaminated broccoli and radish seeds. Food Microbiol. 2015, 51, 10–17. [CrossRef]

53. BenJemaa,M.;Falleh,H.;Saada,M.; Oueslati, M.; Snoussi, M.; Ksouri, R.Thymuscapitatusessentialoilamelioratespasteurization efficiency. J. Food Sci. Technol. 2018, 55, 3446–3452. [CrossRef]

54. Asensio, C.M.; Quiroga, P.R.; Huang, Q.; Nepote, V.; Grosso, N.R. Fatty acids, volatile compounds and microbial quality preservation with an oregano nanoemulsion to extend the shelf life of hake (Merluccius hubbsi) burgers. Int. J. Food Sci. Technol. 2018, 54, 149–160. [CrossRef]

55. Jo,Y.-J.; Chun, J.-Y.; Kwon, Y.-J.; Min, S.-G.; Hong, G.-P.; Choi, M.-J. Physical and antimicrobial properties of trans-cinnamaldehyde nanoemulsions in water melon juice. LWT-Food Sci. Technol. 2015, 60, 444–451. [CrossRef]

56. Moraes-Lovison, M.; Marostegan, L.F.; Peres, M.S.; Menezes, I.F.; Ghiraldi, M.; Rodrigues, R.A.; Fernandes, A.M.; Pinho, S.C. Nanoemulsions encapsulating oregano essential oil: Production, stability, antibacterial activity and incorporation in chicken pâté. LWT 2017, 77, 233–240. [CrossRef]
57. Acevedo-Fani, A.; Salvia-Trujillo, L.; Rojas-Graü, M.A.; Martín-Belloso, O. Edible films from essential-oil-loaded nanoemulsions: Physicochemical characterization and antimicrobial properties. Food Hydrocoll. 2015, 47, 168–177. [CrossRef]

58. Liang, R.; Xu, S.; Shoemaker, C.F.; Li, Y.; Zhong, F.; Huang, Q. Physical and Antimicrobial Properties of Peppermint Oil Nanoemulsions. J. Agric. Food Chem. 2012, 60, 7548–7555. [CrossRef]

59. Shah, B.; Davidson, P.M.; Zhong, Q. Nanodispersed eugenol has improved antimicrobial activity against Escherichia coli O157:H7 and Listeria monocytogenes in bovine milk. Int. J. Food Microbiol. 2013, 161, 53–59. [CrossRef]

60. Hilbig, J.; Ma, Q.; Davidson, P.M.; Weiss, J.; Zhong, Q. Physical and antimicrobial properties of cinnamon bark oil co nanoemulsified by lauric arginate and Tween 80. Int. J. Food Microbiol. 2016, 233, 52–59. [CrossRef]

61. Keivani Nahr, F.; Ghanbarzadeh, B.; Hamishehkar, H.; Samadi Kafil, H. Food grade nanostructured lipid carrier for cardamom essential oil: Preparation, characterization and antimicrobial activity. J. Funct. Foods 2018, 40, 1–8. [CrossRef]

62. Alparslan, Y.; Baygar, T. Effect of Chitosan Film Coating Combined with Orange Peel Essential Oil on the Shelf Life of Deepwater Pink Shrimp. Food Bioprocess Technol. 2017, 10, 842–853. [CrossRef]

63. Cui, H.; Bai, M.; Li, C.; Liu, R.; Lin, L. Fabrication of chitosan nanofibers containing tea tree oil liposomes against Salmonella spp. in chicken. LWT 2018, 96, 671–678. [CrossRef]

64. Yuan, G.; Chen, X.; Li, D. Chitosan films and coatings containing essential oils: The antioxidant and antimicrobial activity, and application in food systems. Food Res. Int. 2016, 89, 117–128. [CrossRef]

65. Amiri, N.; Afsharmanesh, M.; Salarmoini, M.; Meimandipour, A.; Hosseini, S.; Ebrahimnejad, H. Effects of nanoencapsulated cumin essential oil as an alternative to the antibiotic growth promoter in broiler diets. J. Appl. Poult. Res. 2020, 29, 875–885. [CrossRef]

66. Soltanzadeh, M.; Peighambardoust, S.H.; Ghanbarzadeh, B.; Mohammadi, M.; Lorenzo, J.M. Chitosan nanoparticles encapsulating lemongrass (Cymbopogon commutatus) essential oil: Physicochemical, structural, antimicrobial and in-vitro release properties. Int. J. Biol. Macromol. 2021, 192, 1084–1097. [CrossRef]

67. Sotelo-Boyás, M.E.; Correa-Pacheco, Z.N.; Bautista-Baños, S.; Corona-Rangel, M.L. Physicochemical characterization of chitosan nanoparticles and nanocapsules incorporated with lime essential oil and their antibacterial activity against food-borne pathogens. LWT 2017, 77, 15–20. [CrossRef]

68. Yousefi, M.; Mohammadi, V.G.; Shadnoush, M.; Khorshidian, N.; Mortazavian, A.M. Zingiber officinale essential oil-loaded chitosan-tripolyphosphate nanoparticles: Fabrication, characterization and in-vitro antioxidant and antibacterial activities. Food Sci. Technol. Int. 2021, 108201322110409. [CrossRef]

69. Hadidi, M.; Pouramin, S.; Adinepour, F.; Haghani, S.; Jafari, S.M. Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities. Carbohydr. Polym. 2020, 236, 116075. [CrossRef]

70. Frank, K.; Garcia, C.V.; Shin, G.H.; Kim, J.T. Alginate Biocomposite Films Incorporated with Cinnamon Essential Oil Nanoemul sions: Physical, Mechanical, and Antibacterial Properties. Int. J. Polym. Sci. 2018, 2018, 1519407. [CrossRef]

71. Vahedikia, N.; Garavand, F.; Tajeddin, B.; Cacciotti, I.; Jafari, S.M.; Omidi, T.; Zahedi, Z. Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes. Colloids Surf. B Biointerfaces 2019, 177, 25–32. [CrossRef]

72. Beyki, M.; Zhaveh, S.; Khalili, S.T.; Rahmani-Cherati, T.; Abollahi, A.; Bayat, M.; Tabatabaei, M.; Mohsenifar, A. Encapsulation of Mentha piperita essential oils in chitosan-cinnamic acid nanogel with enhanced antimicrobial activity against Aspergillus flavus. Ind. Crop. Prod. 2014, 54, 310–319. [CrossRef]

73. Wen,P.; Zhu, D.-H.; Feng, K.; Liu, F.-J.; Lou, W.-Y.; Li, N.; Zong, M.-H.; Wu, H. Fabrication of electrospun polylactic acid nanofilm incorporating cinnamon essential oil/-cyclodextrin inclusion complex for antimicrobial packaging. Food Chem. 2016, 196, 996–1004. [CrossRef] [PubMed]

74. Ellahi, H.; Sadrabad, E.K.; Hekmatimoghaddam, S.; Jebali, A.; Sarmast, E.; Mohajeri, F.A. Application of essential oil of Pistacia atlantica Gum, polypropylene and silica nanoparticles as a new milk packaging. Food Sci. Nutr. 2020, 8, 4037–4043. [CrossRef]

75. Hosseinzadeh, S.; Partovi, R.; Talebi, F.; Babaei, A. Chitosan/TiO2 nanoparticle/Cymbopogon citratus essential oil film as food packaging material: Physico-mechanical properties and its effects on microbial, chemical, and organoleptic quality of minced meat during refrigeration. J. Food Process. Preserv. 2020, 44, e14536. [CrossRef]

76. Lin, L.; Zhu, Y.; Cui, H. Electrospun thyme essential oil/gelatin nanofibers for active packaging against Campylobacter jejuni in chicken. LWT 2018, 97, 711–718. [CrossRef]

77. Nut ,ă, D.; Limban, C.; Chirit ,ă, C.; Chifiriuc, M.; Costea, T.; Ionit ,ă, P.; Nicolau, I.; Zarafu, I. Contribution of Essential Oils to theFight against Microbial Biofilms—A Review. Processes 2021, 9, 537. [CrossRef]

78. Rossi, C.; Chaves-López, C.; Serio, A.; Casaccia, M.; Maggio, F.; Paparella, A. Effectiveness and mechanisms of essential oils for biofilm control on food-contact surfaces: An updated review. Crit. Rev. Food Sci. Nutr. 2020, 62, 2172–2191. [CrossRef]

79. Mishra, A.P.; Devkota, H.P.; Nigam, M.; Adetunji, C.O.; Srivastava, N.; Saklani, S.; Shukla, I.; Azmi, L.; Shariati, M.A.; Coutinho, H.D.M.; et al. Combination of essential oils in dairy products: A review of their functions and potential benefits. LWT-Food Sci.Technol. 2020, 133, 110116. [CrossRef]

80. Maurya, A.; Prasad, J.; Das, S.; Dwivedy, A.K. Essential Oils and Their Application in Food Safety. Front. Sustain. Food Syst. 2021,5, 133. [CrossRef]

81. Ferreira, C.D.; Nunes, I.L. Oil nanoencapsulation: Development, application, and incorporation into the food market. Nanoscale Res. Lett. 2019, 14, 9. [CrossRef]

82. Duong,V.-A.; Nguyen, T.-T.-L.; Maeng, H.-J. Preparation of solid lipid nanoparticles and nanostructured lipid carriers for drug delivery and the effects of preparation parameters of solvent injection method. Molecules 2020, 25, 4781. [CrossRef] [PubMed]

83. Bazzaz, B.F.; Khameneh, B.; Namazi, N.; Iranshahi, M.; Davoodi, D.; Golmohammadzadeh, S. Solid lipid nanoparticles carrying Eugenia caryophyllata essential oil: The novel nanoparticulate systems with broad-spectrum antimicrobial activity. Lett. Appl. Microbiol. 2018, 66, 506–513. [CrossRef] [PubMed]

84. Abreu, F.O.M.S.; de Oliveira, E.F.; Paula, H.C.B.; de Paula, R.C.M. Chitosan/cashew gum nanogels for essential oil encapsulation. Carbohydr. Polym. 2012, 89, 1277–1282. [CrossRef] [PubMed]

85. Linklater, D.P.; Baulin, V.A.; Le Guével, X.; Fleury, J.; Hanssen, E.; Nguyen, T.H.P.; Juodkazis, S.; Bryant, G.; Crawford, R.J.; Stoodley, P.; et al. Antibacterial Action of Nanoparticles by Lethal Stretching of Bacterial Cell Membranes. Adv. Mater. 2020, 32, e2005679. [CrossRef]

86. Wu, Y.; Bai, J.; Zhong, K.; Huang, Y.; Qi, H.; Jiang, Y.; Gao, H. Antibacterial Activity and Membrane-Disruptive Mechanism of 3-p-trans-Coumaroyl-2-hydroxyquinic Acid, a Novel Phenolic Compound from Pine Needles of Cedrus deodara, against Staphylococcus aureus. Molecules 2016, 21, 1084. [CrossRef]

87. Doost, A.S.; Nasrabadi, M.N.; Kassozi, V.; Nakisozi, H.; Van der Meeren, P. Recent advances in food colloidal delivery systems for essential oils and their main components. Trends Food Sci. Technol. 2020, 99, 474–486. [CrossRef]

88. Lopez-Romero, J.C.; González-Ríos, H.; Borges, A.; Simões, M. Antibacterial Effects and Mode of Action of Selected Essential Oils Components against Escherichia coli and Staphylococcus aureus. Evid. Based Complement. Altern. Med. 2015, 2015, 795435. [CrossRef]

89. Helander, I.M.; Alakomi, H.-L.; Latva-Kala, K.; Koski, P. Polyethyleneimine is an effective permeabilizer of Gram-negative bacteria. Microbiology 1997, 143, 3193–3199. [CrossRef]

90. Yang, L.; Zhan, C.; Huang, X.; Hong, L.; Fang, L.; Wang, W.; Su, J. Durable Antibacterial Cotton Fabrics Based on Natural Borneol-Derived Anti-MRSA Agents. Adv. Health Mater. 2020, 9, e2000186. [CrossRef]

91. Cristani, M.; D’Arrigo, M.; Mandalari, G.; Castelli, F.; Sarpietro, M.G.; Micieli, D.; Venuti, V.; Bisignano, G.; Saija, A.A.; Trombetta, D. Interaction of Four Monoterpenes Contained in Essential Oils with Model Membranes: Implications for Their Antibacterial Activity. J. Agric. Food Chem. 2007, 55, 6300–6308. [CrossRef]

92. Gabel, C.V.; Berg, H.C. The speed of the flagellar rotary motor of Escherichia coli varies linearly with protonmotive force. Proc. Natl. Acad. Sci. USA 2003, 100, 8748–8751. [CrossRef] [PubMed]

93. DiPasqua, R.; Betts, G.; Hoskins, N.; Edwards, M.; Ercolini, D.; Mauriello, G. Membrane Toxicity of Antimicrobial Compounds from Essential Oils. J. Agric. Food Chem. 2007, 55, 4863–4870. [CrossRef] [PubMed]

94. Nazzaro, F.; Fratianni, F.; De Martino, L.; Coppola, R.; De Feo, V. Effect of Essential Oils on Pathogenic Bacteria. Pharmaceuticals 2013, 6, 1451–1474. [CrossRef] [PubMed]

95. DiStefano, V.; Schillaci, D.; Cusimano, M.G.; Rishan, M.; Rashan, L. In Vitro Antimicrobial Activity of Frankincense Oils from Boswellia sacra Grown in Different Locations of the Dhofar Region (Oman). Antibiotics 2020, 9, 195. [CrossRef] [PubMed]

96. Gómez-Estaca, J.; de Lacey, A.L.; López-Caballero, M.E.; Gómez-Guillén, M.C.; Montero, P. Biodegradable gelatin–chitosan films incorporated with essential oils as antimicrobial agents for fish preservation. Food Microbiol. 2010, 27, 889–896. [CrossRef]

97. Lin, L.; Mao, X.; Sun, Y.; Rajivgandhi, G.; Cui, H. Antibacterial properties of nanofibers containing chrysanthemum essential oil and their application as beef packaging. Int. J. Food Microbiol. 2018, 292, 21–30. [CrossRef]

98. Zarzycki, P.K.; ena Fenert, B.; Głód, B.K. 17—Cyclodextrins-based nanocomplexes for encapsulation of bioactive compounds in food, cosmetics, and pharmaceutical products: Principles of supramolecular complexes formation, their influence on the antioxidative properties of target chemicals, and rec. In Encapsulations; Grumezescu, A.M., Ed.; Nanotechnology in the Agri-Food Industry; Academic Press: Cambridge, MA, USA, 2016; pp. 717–767, ISBN 978-0-12-804307-3.

99. European Chemicals Agency. Essential Oils-ECHA; ECHA: Helsinki, Finland, 2016.

100. Turek, C.; Stintzing, F.C. Stability of Essential Oils: A Review. Compr. Rev. Food Sci. Food Saf. 2013, 12, 40–53. [CrossRef]

101. Ilmi, A.; Praseptiangga, D.; Muhammad, D.R.A. Sensory Attributes and Preliminary Characterization of Milk Chocolate Bar Enriched with Cinnamon Essential Oil. IOP Conf. Ser. Mater. Sci. Eng. 2017, 193, 012031. [CrossRef]

102. Ghaderi-Ghahfarokhi, M.; Barzegar, M.; Sahari, M.A.; Azizi, M.H. Nanoencapsulation Approach to Improve Antimicrobial and Antioxidant Activity of Thyme Essential Oil in Beef Burgers During Refrigerated Storage. Food Bioprocess Technol. 2016, 9,1187–1201. [CrossRef]

103. Borthakur, P.; Boruah, P.K.; Sharma, B.; Das, M.R. Nanoemulsion: Preparation and its application in food industry. In Emulsions; Academic Press: Cambridge, MA, USA, 2016; pp. 153–191. [CrossRef]

104. Pathak, M. Nanoemulsions and Their Stability for Enhancing Functional Properties of Food Ingredients. In Nanotechnology Applications in Food; Academic Press: Cambridge, MA, USA, 2017; pp. 87–106. [CrossRef]

105. McClements, D.J. Nanoparticle-and Microparticle-Based Delivery Systems: Encapsulation, Protection and Release of Active Compounds; CRCPress: Boca Raton, FL, USA, 2019; ISBN 9781447167655.

106. Ribeiro, L.N.M.; Alcântara, A.C.S.; Da Silva, G.H.R.; Franz-Montan, M.; Nista, S.V.G.; Castro, S.R.; Couto, V.M.; Guilherme, V.A.; DePaula, E. Advances in Hybrid Polymer-Based Materials for Sustained Drug Release. Int. J. Polym. Sci. 2017, 2017, 1231464. [CrossRef]

107. Forensic Science. In Handbook of Analytical Separations; Elsevier: Amsterdam, The Netherlands, 2008.

108. de Paula, E.; Oliveira, J.D.; de Lima, F.F.; de Morais Ribeiro, L.N. Liposome-Based Delivery of Therapeutic Agents. In Controlled Drug Delivery Systems; CRC Press: Boca Raton, FL, USA, 2020; pp. 299–324.

109. Taylor, T.M.; Weiss, J.; Davidson, P.M.; Bruce, B.D. Liposomal Nanocapsules in Food Science and Agriculture. Crit. Rev. Food Sci. Nutr. 2005, 45, 587–605. [CrossRef] [PubMed]

110. Hammoud, Z.; Gharib, R.; Fourmentin, S.; Elaissari, A.; Greige-Gerges, H. New findings on the incorporation of essential oil components into liposomes composed of lipoid S100 and cholesterol. Int. J. Pharm. 2019, 561, 161–170. [CrossRef] [PubMed]

111. Sebaaly, C.; Trifan, A.; Sieniawska, E.; Greige-Gerges, H. Chitosan-Coating Effect on the Characteristics of Liposomes: A Focus on Bioactive Compounds and Essential Oils: A Review. Processes 2021, 9, 445. [CrossRef]

112. Ingle, A.P.; Shende, S.; Gupta, I.; Rai, M. Recent trends in the development of nano-bioactive compounds and delivery systems. In Biotechnological Production of Bioactive Compounds; Elsevier: Amsterdam, The Netherlands, 2019; pp. 409–431. [CrossRef]

113. Singh, Y.; Meher, J.G.; Raval, K.; Khan, F.A.; Chaurasia, M.; Jain, N.K.; Chourasia, M.K. Nanoemulsion: Concepts, development and applications in drug delivery. J. Control. Release 2017, 252, 28–49. [CrossRef]

114. Jemaa, M.B.; Falleh, H.; Serairi, R.; Neves, M.A.; Snoussi, M.; Isoda, H.; Nakajima, M.; Ksouri, R. Nanoencapsulated Thymus capitatus essential oil as natural preservative. Innov. Food Sci. Emerg. Technol. 2018, 45, 92–97. [CrossRef]

115. Müller, R.H.; Alexiev, U.; Sinambela, P.; Keck, C.M. Nanostructured Lipid Carriers (NLC): The Second Generation of Solid Lipid Nanoparticles. In Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement; Dragicevic, N., Maibach, H.I., Eds.; Springer: Berlin/Heidelberg, Germany, 2016; pp. 161–185, ISBN 978-3-662-45012-3.

116. Trevaskis, N.L.; Charman, W.N.; Porter, C.J.H. Lipid-based delivery systems and intestinal lymphatic drug transport: A mechanistic update. Adv. Drug Deliv. Rev. 2008, 60, 702–716. [CrossRef]

117. Pink, D.L.; Loruthai, O.; Ziolek, R.M.; Wasutrasawat, P.; Terry, A.E.; Lawrence, M.J.; Lorenz, C.D. On the Structure of Solid Lipid Nanoparticles. Small 2019, 15, e1903156. [CrossRef]

118. Azar, F.A.N.; Pezeshki, A.; Ghanbarzadeh, B.; Hamishehkar, H.; Mohammadi, M. Nanostructured lipid carriers: Promising delivery systems for encapsulation of food ingredients. J. Agric. Food Res. 2020, 2, 100084. [CrossRef]

119. Katouzian, I.; Esfanjani, A.F.; Jafari, S.M.; Akhavan, S. Formulation and application of a new generation of lipid nano-carriers for the food bioactive ingredients. Trends Food Sci. Technol. 2017, 68, 14–25. [CrossRef]

120. Shegokar, R.; Athawale, R.; Kurup, N.; Yang, R.; Chougule, M.B. Lipid-Based Nanoparticles for Targeted Drug Delivery of Anticancer Drug. In Nanotechnology-Based Approaches for Targeting and Delivery of Drugs and Genes; Academic Press: Cambridge,
MA,USA,2017; pp. 287–321.

121. Alanchari, M.; Mohammadi, M.; Yazdian, F.; Ahangari, H.; Ahmadi, N.; Emam-Djomeh, Z.; Homayouni-Rad, A.; Ehsani, A. Optimization and antimicrobial efficacy of curcumin loaded solid lipid nanoparticles against foodborne bacteria in hamburger patty. J. Food Sci. 2021, 86, 2242–2254. [CrossRef]

122. Diab, R.; Khameneh, B.; Joubert, O.; Duval, R. Insights in Nanoparticle-Bacterium Interactions: New Frontiers to Bypass Bacterial Resistance to Antibiotics. Curr. Pharm. Des. 2015, 21, 4095–4105. [CrossRef] [PubMed]

123. Montoto, S.S.; Muraca, G.; Ruiz, M.E. Solid Lipid Nanoparticles for Drug Delivery: Pharmacological and Biopharmaceutical Aspects. Front. Mol. Biosci. 2020, 7, 587997. [CrossRef] [PubMed]

124. Ribeiro, L.N.; Alcantara, A.C.; Franz-Montan, M.; Couto, V.M.; Nista, S.V.; de Paula, E. Nanostructured organic-organic bio-hybrid delivery systems. In Biomedical Application of Nanoparticles; Elsevier: Amsterdam, The Netherlands, 2019; pp. 341–374. [CrossRef]

125. Khezri, K.; Farahpour, M.R.; Rad, S.M. Efficacy of Mentha pulegium essential oil encapsulated into nanostructured lipid carriers as an in vitro antibacterial and infected wound healing agent. Colloids Surf. A Physicochem. Eng. Asp. 2020, 589, 124414. [CrossRef]

126. Verma, A.; Uzun, O.; Hu, Y.; Hu, Y.; Han, H.-S.; Watson, N.; Chen, S.; Irvine, D.J.; Stellacci, F. Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. Nat. Mater. 2008, 7, 588–595. [CrossRef] [PubMed]

127. Siek, M.; Kandere-Grzybowska, K.; Grzybowski, B.A. Mixed-Charge, pH-Responsive Nanoparticles for Selective Interactions with Cells, Organelles, and Bacteria. Acc. Mater. Res. 2020, 1, 188–200. [CrossRef]

128. Esmaeili, A.; Gholami, M. Optimization and preparation of nanocapsules for food applications using two methodologies. Food Chem. 2015, 179, 26–34. [CrossRef]

129. Rezaeinia, H.; Ghorani, B.; Emadzadeh, B.; Tucker, N. Electrohydrodynamic atomization of Balangu (Lallemantia royleana) seed gumfor the fast-release of Mentha longifolia L. essential oil: Characterization of nano-capsules and modeling the kinetics of release. Food Hydrocoll. 2019, 93, 374–385. [CrossRef]

130. European Commission. European Commission Commission Regulation (EU) No 10/2011; Official Journal of the European Union: Brussels, Belgium, 2011.

131. Chiriac, A.; Rusu, A.; Nita, L.; Chiriac, V.; Neamtu, I.; Sandu, A. Polymeric Carriers Designed for Encapsulation of Essential Oils with Biological Activity. Pharmaceutics 2021, 13, 631. [CrossRef]

132. Petitjean, M.; García-Zubiri, I.X.; Isasi, J.R. History of cyclodextrin-based polymers in food and pharmacy: A review. Environ. Chem. Lett. 2021, 19, 3465–3476. [CrossRef]

133. Szente, L.; Szejtli, J. Cyclodextrins as food ingredients. Trends Food Sci. Technol. 2004, 15, 137–142. [CrossRef]

134. Marques, C.S.; Carvalho, S.G.; Bertoli, L.D.; Villanova, J.C.O.; Pinheiro, P.F.; dos Santos, D.C.M.; Yoshida, M.I.; de Freitas, J.C.C.; Cipriano, D.F.; Bernardes, P.C.-Cyclodextrin inclusion complexes with essential oils: Obtention, characterization, antimicrobial activity and potential application for food preservative sachets. Food Res. Int. 2019, 119, 499–509. [CrossRef] [PubMed]

135. Cramer, F.; Freudenberg, K.; Plieninger, H. Process for the Preparation of Inclusion Compounds of Physiologically Active Organic Compounds. Patent No. 895769, 5 November 1953.

136. Mujtaba, M.; Khawar, K.M.; Camara, M.C.; Carvalho, L.B.; Fraceto, L.F.; Morsi, R.E.; Elsabee, M.Z.; Kaya, M.; Labidi, J.; Ullah, H.; et al. Chitosan-based delivery systems for plants: A brief overview of recent advances and future directions. Int. J. Biol. Macromol. 2020, 154, 683–697. [CrossRef] [PubMed]

137. Bonetti, F.M.R.; de Paula, E.; Fonseca, B.B.; da Silva, G.R.; da Silva, L.S.S.; de Moura, L.D.; Breitkreitz, M.C.; da Silva, G.H.R.; Ribeiro, L.N.D.M. Hybrid Nanobeads for Oral Indomethacin Delivery. Pharmaceutics 2022, 14, 583. [CrossRef] [PubMed]

138. Möller, H.; Grelier, S.; Pardon, A.P.; Coma, V. Antimicrobial and Physicochemical Properties of Chitosan HPMC-Based Films. J. Agric. Food Chem. 2004, 52, 6585–6591. [CrossRef] [PubMed]

139. Li, Z.; Lin, S.; An, S.; Liu, L.; Hu, Y.; Wan, L. Preparation, characterization and anti-aflatoxigenic activity of chitosan packaging films incorporated with turmeric essential oil. Int. J. Biol. Macromol. 2019, 131, 420–434. [CrossRef]

140. Xavier, L.O.; Sganzerla, W.G.; Rosa, G.B.; da Rosa, C.G.; Agostinetto, L.; Veeck, A.P.D.L.; Bretanha, L.C.; Micke, G.A.; Costa, M.D.; Bertoldi, F.C.; et al. Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation. Int. J. Biol. Macromol. 2020, 169, 183–193. [CrossRef]

141. Sotelo-Boyás, M.; Correa-Pacheco, Z.; Bautista-Baños, S.; Gómez, Y.G.Y. Release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria. Int. J. Biol. Macromol. 2017, 103, 409–414.[CrossRef]

142. Ebrahimzadeh, S.; Bari, M.R.; Hamishehkar, H.; Kafil, H.S.; Lim, L.-T. Essential oils-loaded electrospun chitosan-poly(vinyl alcohol) nonwovens laminated on chitosan film as bilayer bioactive edible films. LWT 2021, 144, 111217. [CrossRef]

143. González, R.J.; Sampedro, F.; Feirtag, J.M.; Sánchez-Plata, M.X.; Hedberg, C.W. Prioritization of Chicken Meat Processing Interventions on the Basis of Reducing the Salmonella Residual Relative Risk. J. Food Prot. 2019, 82, 1575–1582. [CrossRef]

144. Vergis, J.; Gokulakrishnan, P.; Agarwal, R.K.; Kumar, A. Essential Oils as Natural Food Antimicrobial Agents: A Review. Crit. Rev. Food Sci. Nutr. 2013, 55, 1320–1323. [CrossRef]

145. Quintavalla, S.; Vicini, L. Antimicrobial food packaging in meat industry. Meat Sci. 2002, 62, 373–380. [CrossRef] 146. Singh, T.; Shukla, S.; Kumar, P.; Wahla, V.; Bajpai, V.K.; Rather, I.A. Application of Nanotechnology in Food Science: Perception and Overview. Front. Microbiol. 2017, 8, 1501. [CrossRef] [PubMed]

147. Anvar, A.A.; Ahari, H.; Ataee, M. Antimicrobial Properties of Food Nanopackaging: A New Focus on Foodborne Pathogens. Front. Microbiol. 2021, 12, 690706. [CrossRef] [PubMed]

148. Sung, S.-Y.; Sin, L.T.; Tee, T.-T.; Bee, S.-T.; Rahmat, A.; Rahman, W.; Tan, A.-C.; Vikhraman, M. Antimicrobial agents for food packaging applications. Trends Food Sci. Technol. 2013, 33, 110–123. [CrossRef]

149. Asensio, C.M.; Quiroga, P.R.; Al-Gburi, A.; Huang, Q.; Grosso, N.R. Rheological Behavior, Antimicrobial and Quorum Sensig Inhibition Study of an Argentinean Oregano Essential Oil Nanoemulsion. Front. Nutr. 2020, 7, 569913. [CrossRef]

150. FDA. Title 21 Code of Federal Regulations; Food and Drug: Silver Spring, MD, USA, 2016; pp. 1–711.

151. Ghaderi-Ghahfarokhi, M.; Barzegar, M.; Sahari, M.; Gavlighi, H.A.; Gardini, F. Chitosan-cinnamon essential oil nano-formulation: Application as a novel additive for controlled release and shelf life extension of beef patties. Int. J. Biol. Macromol. 2017, 102, 19–28. [CrossRef]

152. Pateiro, M.; Barba, F.J.; Domínguez, R.; Sant’Ana, A.S.; Khaneghah, A.M.; Gavahian, M.; Gómez, B.; Lorenzo, J.M. Essential oils as natural additives to prevent oxidation reactions in meat and meat products: A review. Food Res. Int. 2018, 113, 156–166. [CrossRef]

153. Benchaar, C.; Greathead, H. Essential oils and opportunities to mitigate enteric methane emissions from ruminants. Anim. Feed Sci. Technol. 2011, 166–167, 338–355. [CrossRef]

154. Licon, C.C.; Moro, A.; Librán, C.M.; Molina, A.M.; Zalacain, A.; Berruga, M.I.; Carmona, M. Volatile Transference and Antimicrobial Activity of Cheeses Made with Ewes’ Milk Fortified with Essential Oils. Foods 2020, 9, 35. [CrossRef]

155. Liu, J.; Zhu, Y.; Jay-Russell, M.; Lemay, D.G.; Mills, D.A. Reservoirs of antimicrobial resistance genes in retail raw milk. Microbiome 2020, 8, 99. [CrossRef]

156. Tóth, A.G.; Csabai, I.; Krikó, E.; T˝ozsér, D.; Maróti, G.; Patai, Á.V.; Makrai, L.; Szita, G.; Solymosi, N. Antimicrobial resistance genes in raw milk for human consumption. Sci. Rep. 2020, 10, 7464. [CrossRef] [PubMed]

157. Xue, J.; Davidson, P.M.; Zhong, Q. Inhibition of Escherichia coli O157:H7 and Listeria monocytognes growth in milk and cantaloupe juice by thymol nanoemulsions prepared with gelatin and lecithin. Food Control 2017, 73, 1499–1506. [CrossRef]

158. McClements, D.J.; Das, A.K.; Dhar, P.; Nanda, P.K.; Chatterjee, N. Nanoemulsion-Based Technologies for Delivering Natural Plant-Based Antimicrobials in Foods. Front. Sustain. Food Syst. 2021, 5, 643208. [CrossRef]

159. Al Abbasy, D.W.; Pathare, N.; Al-Sabahi, J.N.; Alam Khan, S. Chemical composition and antibacterial activity of essential oil isolated from Omani basil (Ocimum basilicum Linn.). Asian Pac. J. Trop. Dis. 2015, 5, 645–649. [CrossRef]

160. Rattanachaikunsopon, P.; Phumkhachorn, P. Antimicrobial Activity of Basil (Ocimum basilicum) Oil against Salmonella Enteritidis in Vitro and in Food. Biosci. Biotechnol. Biochem. 2010, 74, 1200–1204. [CrossRef] [PubMed]

161. Thabet, H.M.; Nogaim, Q.A.; Qasha, A.S.; Abdoalaziz, O.; Alnsheme, N. Evaluation of the effects of some plant derived essential oils on shelf life extension of Labneh Evaluation of the effects of some plant derived essential oils on shelf life extension of Labneh. Merit Res. J. Food Sci. Technol. 2014, 2, 8–14.

162. Kholy, W.E.; Aamer, R.A.; Mailam, M.A. Effect of Some Essential Oils on the Quality of UF-Soft Cheese During Storage. Alex. J. Food Sci. Technol. 2017, 14, 13–28. [CrossRef]

163. Tomar, O.; Akarca, G. Effects of Ice Cream Produced with Lemon, Mandarin, and Orange Peel Essential Oils on Some Physico chemical, Microbiological and Sensorial Properties. Kocatepe Vet. J. 2019, 12, 62–70. [CrossRef]

164. Artiga-Artigas, M.; Acevedo-Fani, A.; Martín-Belloso, O. Improving the shelf life of low-fat cut cheese using nanoemulsion-based edible coatings containing oregano essential oil and mandarin fiber. Food Control 2017, 76, 1–12. [CrossRef]

165. Sytar, O.; Hemmerich, I.; Zivcak, M.; Rauh, C.; Brestic, M. Comparative analysis of bioactive phenolic compounds composition from 26 medicinal plants. Saudi J. Biol. Sci. 2018, 25, 631–641. [CrossRef]

166. Kaur, R.; Kaur, L. Encapsulated natural antimicrobials: A promising way to reduce microbial growth in different food systems. Food Control 2020, 123, 107678. [CrossRef]

167. Balta, I.; Brinzan, L.; Stratakos, A.C.; Linton, M.; Kelly, C.; Pinkerton, L.; Corcionivoschi, N. Geraniol and Linalool Loaded Nanoemulsions and Their Antimicrobial Activity. Bull. Univ. Agric. Sci. Vet. Med. Anim. Sci. Biotechnol. 2017, 74, 157–161. [CrossRef]

168. Quik, J.T.K.; Meesters, J.A.J.; Peijnenburg, W.J.G.M.; Brand, W.; Bleeker, E.A.J. Environmental Risk Assessment (ERA) of the Application of Nanoscience and Nanotechnology in the Food and Feed Chain; European Food Safety Authority: Parma, Italy, 2020; Volume 17, ISBN 4870980622.

169. Aswathanarayan, J.B.; Vittal, R.R. Nanoemulsions and Their Potential Applications in Food Industry. Front. Sustain. Food Syst. 2019, 3, 95. [CrossRef]

170. Jeevanandam, J.; Barhoum, A.; Chan, Y.S.; Dufresne, A.; Danquah, M.K. Review on nanoparticles and nanostructured materials: History, sources, toxicity and regulations. Beilstein J. Nanotechnol. 2018, 9, 1050–1074. [CrossRef]

171. Zhang, H.; Chen, S. Nanoparticle-based methods for food safety evaluation. In Evaluation Technologies for Food Quality; Woodhead Publishing: Sawston, UK, 2019; pp. 817–835. [CrossRef]

How do essential oils interact with the bacterial cytoplasmic membrane and what are the consequences of these interactions?

Such interactions can promote stretching and compression forces that generate important modifications and deconfiguration in their structure, directly related to the shape of the NP and that allows a higher affinity of interaction with the membrane, causing diffuse elongation and its rupture, due to a mechanical process, induced by tension. As a result, an exacerbated permeability process can occur that affects cellular metabolism and ion leakage, destroys genetic material, and inhibits bacterial quorum sensing signaling, also promoting the release of microbial cellular contents, leading to the death of the pathogen.

What are some of the potential mechanisms of action of essential oils on microorganisms, and how do they contribute to food safety?

Preventive mechanisms against reactive oxygen species, protein oxidation, and mitochondrial alterations complement the cell damage, promoting injury and interruption of microbial development. Other mechanisms of action include access to the periplasm, adenosine triphosphate ATP alteration and cellular hyperpolarization, modification of the melting point of membrane microenvironment, reduction of the membrane protein interaction, interference in flagella formation, and microbial movement, and alteration of the fatty acid organization of the membrane. Essential oils can act as a powerful tool to reduce bacterial resistance, which is essential for food safety in various food fields like fish, chicken pâté, milk, pork, and beef.

What are the advantages of using Solid Lipid Nanoparticles (SLNs) in food distribution?

SLNs allow to improve the stability of loaded EOs under various adverse conditions, including food processing, heating, high pressure, drying, UV radiation, and bile salt. Such a system was designed for applications in food distribution because it has interesting advantages to be used in this sector, such as the effective uptake of fat-soluble assets, sustained release profile, and long-term stability.

What are the advantages of encapsulating essential oils (EOs) in nanosystems for food industry applications?

The application of EO encapsulated by nanosystems should offer a set of advantages that ensures its use as promising nanostructured formulations in FI application. The use of EOs in the food chain is beneficial to inhibit microorganisms contaminations, which justifies its use.

What regulatory aspects and challenges are associated with the use of nanotechnology-based products in food and feed industries?

According to Brazil, a robust international regulatory framework for the evaluation of nanotechnology-based products is necessary for approval of food and feed containing nanoparticles. Despite the advances already reported, there is no standard protocol for the toxicity testing of nanomaterials that assesses, for instance, the NP excretion mechanisms after digestion. There are still no global regulations for manufacturing, labeling, or in vivo and in vitro toxicity testing, nor for potential environmental harm from nanoparticle residues.

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Essential Oil-Based Nanoparticles as Antimicrobial Agents in the Food Industry

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How do essential oils interact with the bacterial cytoplasmic membrane and what are the consequences of these interactions?

What are some of the potential mechanisms of action of essential oils on microorganisms, and how do they contribute to food safety?

What are the advantages of using Solid Lipid Nanoparticles (SLNs) in food distribution?

What are the advantages of encapsulating essential oils (EOs) in nanosystems for food industry applications?

What regulatory aspects and challenges are associated with the use of nanotechnology-based products in food and feed industries?

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