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Review of the chemistry, metabolism, and dose response of two supplemental methionine sources and the implications in their relative bioefficacy

Published: January 4, 2019
Summary
This review examines the relative bioefficacy of 2-hydroxy-4-(methylthio) butanoic acid (HMTBA) and DL-methionine (DL-Met) which includes chemical, metabolic, nutritional, and statistical aspects of its bioefficacy. The chemical, enzymatic and biological differences and similarities between these two products are explained and the evidence and reasons for HMTBA relative bioefficacy to DL-Met in m...
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Authors:
Mercedes Vazquez-anon
Novus
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gary reznik
Devenish Nutrition
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Influencers who recommended :
Mehran Mehri, Raquel Araujo and 2 more
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Rahat Mobeen
Shahzad Feeds Mill
22 de marzo de 2019

What is the absoption time of HMTBA and DLM? Which product more rapidly absorb in bird and absorbed in how much time? (Normal stay time of feed in GIT is 6.5-7 hours). Which product more appear in feaces?

Luiz Souza
ADM Animal Nutrition
27 de marzo de 2019

Rahat Mobeen
A few good articles for you to understand this issue:
1. Fang et al (2010) Brit J Nutr - Effects of DL-2-hydroxy-4-methylthiobutyrate on the first-pass intestinal metabolism of dietary methionine and its extra-intestinal availability
2. Jendza et al (2011) J Anim Sci - The site of intestinal disappearance of DL-methionine and methionine hydroxy analogue differs in pigs
3. Richards et al (2005) Poul Sci - Comparative in vitro and in vivo absorption of 2-Hydroxy-4(Methylthio) Butanoic Acid and Methionine in the broiler chicken

Roughly, sites and mechanisms of absorption are slightly different, resulting that HMTBA absorption is less influenced by other nutrients than DLM, and is absorbed before DLM in the GIT.
But check the articles and take your conclusions.

Alireza rostamkhani
25 de marzo de 2019

Please revision EFSA 2018 report and dose response test 3 level MHA for broiler and result used for comparison. MHA efficacy not more than 80% equimolar basis (88%*80%=70% potency)

Luiz Souza
ADM Animal Nutrition
27 de marzo de 2019

Alireza rostamkhani
The methodology used for determining the comparative Biological Value where this lower "potency" was found is wrong and it was revised by the main research group.
Please, check on "Kratzer et al (2006) Poul Sci - Appropriate statistical methods to compare dose responses of methionine sources".
EFSA will review their position in the future, since they also have used an obsolete method, based on a misconception.
If you look to the market, 75% of US feed industry use liquid HMTBA as TSAA source with efficiency.

Alireza rostamkhani
28 de marzo de 2019

Luiz W. O. Souza
Kinetics of Hydrolysis of Dimeric and Trimeric Methionine Hydroxy Analogue
Free Acid under Physiological Conditions of pH and Temperature
Hans G. Koban* and Edgar Koberstein
Liquid feed supplements baaed on DL-methionine hydroxy analogue free acid (DL-MHAfree acid,
~~-2-hydroxy-4-(methylthio)butyric acid) contain considerable amounts of oligomeric MHA free acids.
As a first approach to physiological decomposition the hydrolysis of dimeric and trimeric MHA free
acid in dilute hydrochloric acid solution is investigated. By use of HPLC it is possible to follow the
changes in concentration of all species. The hydrolysis reactions are found to be first order with respect
to the oligomer concentration. The rate constant increases with HC1 concentration and temperature
(activation energy for dimeric MHA free acid: 72 kJ/mol). The rate constants obtained at 37 OC in
0.1 N aqueous HC1correspond to half-lives of 1.8 and 1.6 days for dimeric and trimeric MHA free acid,
respectively. The results indicate that oligomeric MHA free acids show rather slow hydrolysis under
physiological conditions of pH and temperature.

Luiz Souza
ADM Animal Nutrition
28 de marzo de 2019

Alireza rostamkhani

Please, see also:

1. Martin-Venegas et al (2006) Poul Sci - Oligomers Are Not the Limiting Factor in the Absorption of DL-2-Hydroxy-4-(methylthio)butanoic Acid in the Chicken Small Intestine

2. Martin-Venegas et al (2008) Poul Sci - Partial Na+ dependence of DL-2-Hydroxy-4-(Methylthio)Butanoic Acid uptake in the chicken small intestine

3. Martin-Venegas et al (2009) Am J Physiol Cell Physiol - Effect of pH on L- and D-methionine uptake across the apical membrane of Caco-2 cells

4. Martin-Venegas et al (2011) Brit J Nutr - Intestinal cell conversion of DL-2-hydroxy-(4-methylthio)butanoic acid in vitro dietary up-regulation by this methionine precursor

5. Monocarboxylate transporter 1 is up-regulated in Caco-2 cells by the methionine precursor DL-2-hydroxy-(4-methylthio)butanoic acid



Alireza rostamkhani
28 de marzo de 2019

Luiz W. O. Souza
We do not how much price and purity mha used feed industry us. however mha very good source of TSAA for sow and different by DLM in efficacy and recovery rate and mix ability in broiler feed.

Rahat Mobeen
Shahzad Feeds Mill
26 de marzo de 2019
Alireza Sir kindly you e.mail me that article please . rahatmobeen.533@gmail.com I read many researches but i can not find my Question?
Rahat Mobeen
Shahzad Feeds Mill
5 de abril de 2019

Luiz W . Dear I also go through the these papers, but I cannot find my question.
No doubt liquid Methionine is a good source and have more share in market.

Luiz Souza
ADM Animal Nutrition
8 de abril de 2019
Rahat Mobeen Please, send your contact to lwsouza@dr.com. I will send papers discussing absorption with highlights where I think address the topic, for your own conclusion on this issue. Best regards, Luiz
Mehran Mehri
11 de agosto de 2023

Rahat Mobeen
The most important problem dealing with the estimation of the RBV on MHA is the inappropraite statistical approach used in most studies as Luiz noted above. Please check out "Kratzer et al (2006) Poul Sci - Appropriate statistical methods to compare dose responses of methionine sources".
In most studies, the common plateau has been fiited for both MHA and DLM, which is incorrect approach and these two sources of Met follow different pattern to reach plateau, so separate plateau should be fitted when estimating the RBV for HMA. Kratzer et al., (2006) showed that separate plateau resulted in better RBV for HMA.

Alireza rostamkhani
15 de agosto de 2023
According to EFSA 2018 report we designed conducted an experiment comparing MHA and DLM in practical farm condition with 6000 broilers ( 49 days trial ).A broiler house was divided in two equal parts , each ˜c3000 birds. We replaced 65 part of DLMET with 100 part MHA in this trial. Results indicated that body weight and FCR were the same in both groups confirming 65% RBV of MHA.
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