Explore

Communities in English

Advertise on Engormix

Effect of in ovo injection of raffinose on growth performance and gut health parameters of broiler chicken

Published: September 9, 2020
By: J. D. Berrocoso, R. Kida, A. K. Singh, Y. S. Kim, and R. Jha. / Department of Human Nutrition, Food and Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Summary

ABSTRACT

The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers. A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents). The RFO solution was injected into the air sac on d 12 of incubation. In total 144 birds were fed a standard diet and management and sacrificed at d 21 post hatch for collection of samples. Total RNA was extracted from the small intestine, and RT-qPCR was performed to quantify mRNA levels of marker genes of immune cells. Injection of RFO had no significant effect (P > 0.05) on d one body weight of chicks. On d 21, the relative weight of the proventriculus, drumstick, breast, and gizzard was not affected (P > 0.05) by RFO. On hatch d, the villus height increased linearly (P < 0.01) with an increasing dose of RFO. Also, an increasing dose of RFO increased the villus height and villus height:crypt depth ratio (P < 0.05) but did not affect the crypt depth on d 21. The expression levels of CD3 and chB6, which are T cell and B cell marker genes, respectively, were significantly enhanced by high dose RFO (4.5 mg). In conclusion, although an increasing dose of RFO in ovo injection did not significantly influence growth performance or slaughter yield of broilers, RFO has the potential of enhancing ileum mucosa morphology and improving immunity in the small intestine, which are indicators of improved gut health.

Key words: growth performance, in ovo injection, ileum mucosa morphology, prebiotic, immunology.

 

Abstract published in 2017 Poultry Science 96:1573–1580 http://dx.doi.org/10.3382/ps/pew430.

Related topics:
Authors:
Julio Diaz Berrocoso
FARM Faes
Rajesh Jha
University of Hawai
Recommend
Comment
Share
Koot van der Walt
16 de octubre de 2020
How can this technology be used in racing pigeons where a healthy gut is also very important to build immunity at an early age? I would appreciate a discussion on Zoom.
Recommend
Reply
Profile picture
Would you like to discuss another topic? Create a new post to engage with experts in the community.
Featured users in Poultry Industry
Padma Pillai
Padma Pillai
Cargill
United States
Kendra Waldbusser
Kendra Waldbusser
Pilgrim´s
United States
Karen Christensen
Karen Christensen
Tyson
Tyson
PhD, senior director of animal welfare at Tyson Foods
United States
Join Engormix and be part of the largest agribusiness social network in the world.