what is the effect of heat treatment of feed in pelleting incase of long time of steam mixing (180 second at 80 degree) on both clostridia and bacillus
Dr.R.Chanthirasekaran probably will beter answer to your question but based on our experiences from EU markets where high temperature pelleting is commonly used to control microbiological quality of the feed we see that 95[percent] of spores of bacillus subtilis PB6 survive 4 minuts of double connditioning and pelleting at 85C. We not maesured Clostridia survive rate in those conditions.
Clostridium as an anaerobe with its spore forming characteristics is extreme resistance to physical changes and also to chemical agents. Spores of Clostridium perfringens survive in the boiling temperature (100 degree celsius) for more than an hour. Also, the pellet feed is prone for recontamination till it is taken by the animal.
As rightly said by Mr.Robert , Bacillus subtilis PB6 spores survive (95[percent]), the pelleting temperature. The concentration of PB6 offered in the product, meets the demand of the chicken, to control Necrotic enteritis.
Thanks for the details brought to the attention of the esteemed readers. As you have informed rightly, the organism Bacillus subtilis PB6 discovered by Kemin possess the important characteristics which make it unique. They include:
 Derived from the healthy chicken gut
 Has definitive mode of action against Clostridium perfringens by producing antimicrobial substances
 Stimulates the growth of commensals by producing an important substance
 Heat stable, and industry friendly
 Patented product.
 Compatible with commonly used anticoccidials in the feed
More details are available in the EFSA’s scientific opinion.
The article quoted by you in British Poultry Science journal has compared Oregano essential oil and enzymes in its study.