Comparative Efficacy of Three Newcastle Disease Vaccine Strain in Layers

Present study on the evaluation of Newcastle Disease vaccines was conducted in the Poultry farm The University of Agriculture Peshawar. Total 96 layer birds in production were purchased from the local market. Commercial layers ration @ 120 g per bird provided to all birds during the whole experimental period. The study was continued for a period of 6 weeks with one week adaptation period. Before starting the experiment all the birds were screened by HI test and diseased birds were culled. The birds showing nonspecific antibody titers (≤2) in HI were selected. The birds were divided into four major groups i.e. group LS, AV, MK, and Cont i.e. vaccinated with lasota (Hilton Company) VG/GA (Avinue Marial Company) Mukhtaswar (Veterinary Research Institute Peshawar) and control respectively. All the mentioned ND vaccines were purchased from farm and were administered to the birds at dose of 0.5cc subcutaneously wing. The antibody titer was determined by hemagglutination inhibition (HI) test. 1 ml Blood samples were collected for the determination of antibody titer. Blood and eggs samples were collected on the day of vaccination and with week interval post vaccination till day 42. Significant difference was found among all vaccinated groups as compared to control. Significantly higher immune response was observed in group that was vaccinated with Mukhteswar strain vaccine against the Newcastle disease. Among all the vaccines of different strains, it was found that after primary vaccination chickens of the group vaccinated with Mukhtaswar strain vitamin E+selinium produced higher immune response than the chickens of other groups vaccinated with lasota or Avineue strains. Mukhtaswar vaccine showed high response towards transmission from body to eggs. As time elapsed the titer of all vaccine gradually decreases. Birds vaccinated with Mukhtaswar+vitminE+Selinium vaccine showed significantly increase in antibody. The results showed that pullets vaccinated with Mukhtaswar+Vitamin E+Selinium vaccine was observed to have higher egg production as compared to other vaccinated and control groups. It was concluded from the present study that Mukhtaswar vaccine either individually or with supplementation significantly increase the immune response and no effect on egg production.