Efficacy of Ascorbic Acid and Butylated Hydroxylanisole in Amelioration of Aflatoxicosis in Broiler Chickens

The effect of ascorbic acid on chickens fed with aflatoxin has no scientific explanation and is possibly accidental. In 1980-1983, I tested the action of butyloxytoluene, butyloxyanisole and sentoqin. Aflatoxin reduced broiler weight by about 10%, that is, up to 90% of the control. Additives of butyloxytoluene, butyloxyanisole and sentoqin weakened the effect of the toxin. The weight in the experimental groups increased to 98 -99% compared with the control. Acted more effectively with ethoxyquin. That is, from 10% of the action of the toxin, the additives were restored 8-9%. The mechanism of action of each additive is associated with the induction of cytochrome P-450. Its concentration in the liver increased by 2 -2.5 times. Cytochrome P-450 inactivates aflatoxin B1. Cytochrome P-450 measurements were performed and metabolites of aflatoxin B1 in vitro were determined. That is, the action of butyloxyanisole in your experiment is not random and is explained by the mechanism I studied. There are 2-3 such publications in world literature. Mine is in Russian and inaccessible to other scientists. Especially in the 80s, the Internet was not available. Wish you success. You are on the right way and only at the beginning. Study the involvement of cytochrome P-450 in the metabolism of mycotoxins. All works will be original. I no longer work in the laboratory. I am many years old. Sincerely, Prof. V.S. Kryukov

Thank you Prof. V.S. Kryukov for your valuable comment.

Ascorbic acid acts as per the mechanisms of anti-oxidant.