Comparative analysis of microbiota from commercial broiler farms of two integration companies using AGP or chestnut/quebracho polyphenol extracts

Published on: 02/28/2020
Author/s : J. Diaz-Carrasco 1,2; L. Redondo 1; N. Casanova 1; and M. Fernandez-Miyakawa 1,2. / 1 Instituto de Patobiología Veterinaria, CICVyA – INTA, Buenos Aires, Argentina; 2 CONICET, Buenos Aires, Argentina.

The importance of the microbiota in the gastrointestinal tract of animals is recognized to have an important role in host health and productivity. In this study, we sought to assess and characterize the microbiota in the feces of poultry chickens from several farms of 2 different integrators in the same geographical region of South America. These companies were changing AGP program to an AGP-free ...

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February 28, 2020
Nice work.

Wondering if you are planning to try using just total RNA random sequencing instead of 16S variable region. In our hands when we use RNA, we obtain almost complete 16S, 18s and 23S for species level resolution and in some cases and viral RNAs as well. Since you are dealing with RNA random amplified you may obtain a sense of what bacteria/viruses are transcriptionally active.
February 28, 2020
Thank you, Claudio. Yes, we plan to do RNAseq in the future, it would provide additional information that could be very interesting. But its very expensive!
February 28, 2020
We are running 48 samples per Run on an Illumina Myseq. This gives lots of bacterial Ribosomal diversity at a cost of about $100 or less per sample for reagents.
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Utsav Prakash Tiwari
Utsav Prakash Tiwari
Ph.D. in Nutrition
  DURHAM, North Carolina, United States
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