Prevalence and Antimicrobial Resistance of Salmonella Isolated from Workers, Internal Organs and Carcasses at Slaughterhouse, Northeastern Thailand.

Food-borne disease caused by Salmonella spp. is an important public health problem. The antimicrobial resistance causes failure of regular therapy and increases the cost of treatment. Salmonella-free carcasses at the slaughterhouse may not happen because there are many stages where workers, liver, intestine, and pig carcasses can be contaminated.Food-borne disease caused by Salmonella spp. is an important public health problem. The antimicrobial resistance causes failure of regular therapy and increases the cost of treatment. Salmonella-free carcasses at the slaughterhouse may not happen because there are many stages where workers, liver, intestine, and pig carcasses can be contaminated. The objective of this study were to determine antimicrobial resistance of Salmonella spp. isolated from workers, liver, intestine and pig carcasses at slaughterhouse in Northeastern Thailand.

A total of 143 samples from workers, livers, intestines and pig carcasses consisted of were collected 38, 22, 25 and 58, respectively at slaughterhouse, Northeastern Thailand. The samplings were collected during April 2012 to September 2013. All samples were examined for Salmonella spp. isolation and identification by ISO 6597:2002. To assess the prevalence of antimicrobial resistance patterns was done using disk diffusion technique among 10 antimicrobials.

Salmonella spp. contaminated to workers, livers, intestines and pig carcasses were 23.7%, 22.7%, 28.0% and 27.6% respectively. An identified serovar from workers were S. Rissen (45%), S. Stantey (11%), S. Bareilly (11%), S. Hindmarsh (11%), i.4,5,12:i:- (11%) and iv.43:Z4Z23:- (11%); from livers were S. Rissen (20%), S. Weltevreden (20%), S. Panama (20%), S. Kedougou (20%) and S. Gaminara (20%); from intestines were i.4,5,12:i:- (29%), S. Panama (14%), S. Stantey (14%), S. Weltevreden (14%), S. Kedougou (14%) and S. Anatum (14%); from pig carcasses were S. Rissen (56%), S. Weltevreden (19%), S. Stantey (13%), S. Panama (6%) and S. Virchow (6%). Ampicillin was high resistance of Salmonella spp. isolated from workers, livers, intestines and pig carcasses were 75%, 60%, 86% and 75%, respectively.

Contamination of Salmonella spp. isolated from workers, livers, intestines and pig carcasses at slaughterhouse may be due to improper sanitation and hygienic management. The prevention and control of Salmonella spp. contaminated to pork was standard slaughterhouse, good processing, hygiene, sanitation and careful handling from healthy workers. Finally, meat inspection for save to consume.

Disclosure of Interest: None Declared.