How many mycotoxins should be analyzed in Indian Poultry Feeds and Raw Materials?
Published: January 19, 2021
By: Dr. Swamy Haladi / Trouw Nutrition India
The so called Mycotoxins are not a new subject to Indian poultry industry. Perhaps, it is not an overstatement to say that Indian poultry feed millers, integrators and farmers understand mycotoxin challenges much better than their counterparts from the other countries. This is due to the extensive applied research carried on mycotoxins in poultry as well as high occurrence of aflatoxicosis in the field.
Mycotoxins are secondary metabolites of molds and their presence in the raw materials and feeds depends on many factors. Today scientists have discovered more than 500 mycotoxins but all of them may not be of economic significance. Some of these factors include moisture, temperature, relative humidity, insect damage, etc. during plant growth as well as during storage of raw materials and feeds in feed mills.
Rule of Thumb for Mycotoxin Analyses
Given that molds are ubiquitous, it is impossible to find any grains or feeds without one or the other mycotoxins. Since there are more than 500 mycotoxins that are known today, it is practically impossible to analyze all of them. It is well accepted globally that the analyses of feeds and raw materials for aflatoxins, ochratoxins, fumonisins, T-2 toxin, DON (vomitoxin) and zearalenone will provide a fair understanding of potential toxicity in animals. Since mycotoxin analysis can be expensive, the following rule of thumb should be applied while considering mycotoxin analyses;
1. When feed millers need to make a decision about accepting or rejecting incoming raw materials, individual raw materials especially grains should be analyzed for mycotoxins.
2. When bird performance and health is questioned in the field, poultry feed should be analyzed first for mycotoxins. If the feed contains more than accepted levels of mycotoxin/s, then raw materials should be analyzed to ascertain the root cause of the problem.
Types of Molds and Mycotoxins
The molds and mycotoxins that are produced in the field during crop growth are respectively called as “field molds” and “field mycotoxins”.Aspergillus, Penicillium and Fusarium are the most studied field molds (Figure 1 and 2). Aspergillus generally grows in tropical countries such as India while Fusarium molds are capable of growing both in tropical, semi tropical and temperate regions of the world. Penicillium molds are more common in semi tropical regions such as Eastern Europe.
Aspergillus and Penicillium molds are well known to be grown during storage of raw materials and feeds (Figure 3). Storage molds and the mycotoxins these produce create a significant problem in tropical regions such as India.
From Table 1, it can be clearly understood that India being a tropical country (some parts are semi-tropical in winter season) the type of mycotoxins that can be expected in raw materials and feeds are aflatoxins, ochratoxins, cyclopiazonic acid, T-2 toxin, HT-2 toxin, DAS, fumonisins and moniliformin. As mentioned earlier, today Indian poultry industry has the practice of analyzing raw materials and feeds for aflatoxins, ochratoxins, T-2 toxin, DON, zearalenone and fumonisins. This practice may not be relevant to Indian poultry (Table 2) and that raises few questions.
Mycotoxin Survey in India
Many surveys have been conducted over the years on mycotoxin contamination in Indian raw materials and feeds. One such survey (Biomin Mycotoxin Survey 2017, Table 2) clearly shows that the occurrence of fumonisins was the highest (88%) followed by aflatoxins (81%) and ochratoxins (76%).
LD50 Values for Mycotoxins
Mycotoxin toxicity depends on the species of animal in question, age of the animal, type and number of toxins detected, and the concentration of mycotoxins in feed. LD50 values are commonly used in laboratory animals and poultry to determine the severity of toxicity of mycotoxins to a specific species. LD50 (mg/kg body weight) means the amount of single dose of toxin that is required to kill 50% of experimental population. In poultry, such measurements are generally made using one-day-old broiler chickens. Table 3 shows LD50 values for some of the mycotoxins of importance in poultry. According to Table 3, poultry species is more sensitive to ochratoxins followed by T-2 toxin, aflatoxins and DON. Poultry is quite resistant to DON.
Taking into account the kind of mycotoxin analyzed in India today, type of mycotoxins actually detected in survey and LD50 values of mycotoxins in poultry, the following questions can raise. An honest effort has been to answer them with scientific facts.
Why are we analyzing DON and Zearalenone in Indian poultry raw materials and feeds?
Table 2 clearly shows that the concentrations of these two toxins are quite low (76 and 21ppb). Moreover, these two toxins are very less toxic to poultry. Please refer to LD50 value in Table 3. Zearalenone levels as high as 800ppm did not cause much toxicity in poultry (Leeson et al., 199%).
Does the high occurrence of fumonisins in Indian feeds warrant its regular analysis?
The answer is “No” as the concentrations detected are too low (Table 2) to cause toxicity in poultry. Weibking et al., (1993) proposed minimum effective dose of 75ppm (75,000ppb) fumonisins to cause toxicity in poultry. This is about 180 times that of the average fumonisins detected in Indian raw materials and feeds (Table 2).
Do we need to analyze T-2 toxin?
There is no doubt that poultry is sensitive to T-2 toxicity (please see Table 3). However, the concentrations are too low to cause toxicity (Table 2). Given their high toxicity in poultry, it is recommended to analyze T-2 toxin case-by-case basis. This means whenever the birds are experiencing immunity and gut health challenges, the analysis of T-2 toxin in the feedis recommended.
Analysis of Mycotoxins
Rapid analysis of mycotoxins at feed mills is very critical to maintain quality of raw materials and poultry feeds. In this regard, a reliable and relatively cheaper technology has been developed which can analyze mycotoxins in less than 15 minutes.
Conclusions
Mycotoxins continue to pose threat to Indian poultry industry by compromising feed quality and health and performance of broilers, layers and breeders. Quality control measures at feed mill such as analyzing moisture and mycotoxin levels of incoming raw materials will help to accept or reject raw materials. Analysis of feed is warranted only when poor bird performance is reported in the field. Frequent analysis of raw materials is recommended only for aflatoxins and ochratoxins and T-2 toxin analysis can be done case-by-case basis. Taking into consideration the scientific facts, the analysis of poultry raw materials and feeds for DON, zearalenone and fumonisins is not required.
This article was originally published on benisonmedia.com and it is reproduced here with permission from the author.
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Trouw Nutrition
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29 de junio de 2021
Dear Navale, I agree with you that very high levels Fumonisin and Zearalenone concentrations are required to induce toxicity in birds. However, the synergistic effect of these mycotoxins with other mycotoxins, if any, need to be explored.
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Trouw Nutrition
24 de junio de 2021
Dear Navale, testing as many mycotoxins as possible always helps to ascertain toxicity more precisely. But under field conditions, such analysis can be expensive and time consuming. A field miller has to make a quick decision whether to accept or reject raw materials very quickly and he can't analyze all mycotoxins. Scientifically, Aflatoxins, OTA and T-2 are the most toxic mycotoxins to poultry and particularly in Asia we need to focus on analyzing them. If you have resources, please do DON and fumonisins also. But zearalenone is not at all required as the concentration required to cause toxicity in poultry is very high.
If you look at pigs, it is a different story. There you need to focus more on DON, fumonisins and zearalenone for testing. An informed and economical decisions need to be made when comes to mycotoxin analysis. I hope this helps.
Regards
Swamy
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National Chemical Laboratory (NCL)
22 de junio de 2021
Dear Haladi,
As per your study and conclusion analysis of DON, zearalenone and fumonisins in poultry raw materials and food is not required. But in our study we found that these raw materials are highly contaminated with zearalenone and fumonisins which surpassed the legal limits.
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