A survey on mycotoxins detected in corn samples received from Serbia and Bosnia & Herzegovina during August to November 2017
Published: April 12, 2018
By: Marko Vasiljevic, Jog Raj, Jasna Bošnjak Neumüller, Robert Cepela, Igor Pol, Hunor Farkas, PATENT CO
The aim of the corn survey 2017 was to screen corn samples received from Serbia and Bosnia & Herzegovina during August to November 2017. The samples were analysed by UHPLC-based multi-mycotoxin method for the determination and quantitation of all mycotoxins (Aflatoxin B1, B2, G1 and G2, Ochratoxin A, Zearalenone, Deoxynivalenol, Fumonisin B1&B2, T-2 &HT-2 toxins) regulated in feed (EU Directive 2002/32/EC, 2006/576/EC and 2013/165/EU) by liquid chromatography coupled with tandem mass spectrometry. The method is based on “dilute and shoot” principle. It involves two-step extraction and centrifugation of the extracts. To compensate the matrix effects in electrospray ionization, the extracts are mixed with [13C] labelled internal standards for each group of mycotoxins U-[13C17]-AB1, U-[13C15]-DON, U-[13C18]-ZON, U-[13C20]-OTA, U-[13C34]-FB1 and U-[13C24]-T-2 before injection onto LC-MS/MS (Agilent 6460c LC-MS/MS).
- A total of 113 samples were received for analysis at Patent Co., Mišicevo, Serbia.
- 53 % samples were found contaminated with mycotoxins and 28% of these contaminated samples were found to contain more than one mycotoxins.
- Aflatoxin B1 (AB1), Fumonisin B1 (FB1), Fumonisin B2 (FB2) and HT-2 were detected in 13%, 44%, 24% and 8% samples.
- AB1 ranging from 0.59 to 5644 ppb, HT-2 ranging from 9 to 66 ppb, FB2 ranging from 53 to 2540 ppb and FB1 ranging from 48 to 8623 ppb, were detected in corn samples.
Therefore, this survey concludes that the corn harvested in 2017 has high levels of Aflatoxin B1, Fumonisin B1, Fumonisin B2 in Serbia and Bosnia & Herzegovina regions.
This poster was presented at the World Mycotoxin Forum, which was held in Amsterdam, the Netherlands, on 12-14 March 2018.
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3 de octubre de 2018
Dear Sirs,
Could you say what is the total (just for orientation!) corn quantity which represented those 113 samples from Serbia and BiH for UHPLC-based multi-mycotoxin determination?
Does such results justify embedding for example Min-a-zel plus into the mixture in which such maize is installed?
What is total timing for UHPLC-based multi-mycotoxin determination? (from sampling to final results)
What is the analysing price?
Do you have any draft expectations or results for 2018.?
Thanks a lot and best regards,
Do you have any draft forecast or results for 2018.?
6 de septiembre de 2018
Well. I'm very much interested about this article and mycotoxin contamination in food and feed grains. And, our team also initiated projects to analyze toxin contamination levels in samples of food and feed collected from various parts of Ethiopia. However, we are in short of ELISA kits and other columns to undertake as planned. Thus I would like to kindly urge those involved in related studies to help my team to solve the challenges of kits and reagents.
Thanks.
25 de abril de 2018
Poultry farmers have used this maize with proper toxin binders.
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