The Effect of Nano-Selenium Particles and Sodium Selenite on Humoral Immunity Indices of Quails using Foods Contaminated induce with Aflatoxin B1

This experiment was conducted to evaluate the ability of inhibition of aflatoxin B1 by various sources of selenium, comparison between the effect of nano selenium and sodium selenite in humoral immunity on quails. The experiment was performed in a completely randomized design (CRD) using six treatments and four replicates of ten quail chicks per replicate. Two hundred forty quails were divided in six groups vis. control: without aflatoxin B1 and without selenium. Group2: 1ppm aflatoxin B1 and without selenium. Group3: 1 ppm aflatoxin B1 and 0.3 ppm nano selenium. Group4: 1 ppm aflatoxin B1 and 0.3 ppm sodium selenite. Group5: 1 ppm aflatoxin B1 and 0.6 ppm nano selenium. Group6: 1ppm aflatoxin B1 and 0.6 ppm sodium selenite. To evaluate the humoral immunity response 0.2ml of sheep red blood cell (SRBC) solution was injected into breast muscle of quails at days 35 as well as, the injection blood sampling was collected after a week. Newcastle vaccine was injected at days 28 and the antibody was tittered after two weeks. The highest level of titer of antibody against the SRBC solution was related to the group which receiving 0.06 ppm nanoselenium (p<0.01). These results indicated that nano selenium in comparison with selenium selenite can improve humoral immunity and blood biochemical parameters.

Keywords: Aflatoxin, Quail, Selenium, Humoral immunity.