Kari Estes

Dry period heat stress significantly reduces milk yield in the subsequent lactation in cows. Altered methylation patterns in mammary tissues is associated with the decline in milk yield. We hypothesized that as a methyl donor, choline (RPC) supplementation reverses similar patterns of methylation and thus productivity in heat-stressed dams. Late-gestation Holstein cows (n = 51) housed in freestall ...

The TMR stability of rumen-protected lysine (RPL) products has been previously investigated, albeit without a positive control, potentially introducing bias. The objective of this experiment was to evaluate the TMR stability of 6 RPL products using a modified technique that used an unprotected Lys treatment as a positive control. Triplicate Ziploc bags containing RPL (equivalent of 1 g of Lys), no ...

A technique has been established to determine the TMR stability of rumen-protected (RP)-lysine products, but the TMR stability of RPcholine (RPC) products has not yet been explored. The objective of this experiment was to evaluate the TMR stability of 5 RPC products using a modified technique. Triplicate Ziploc bags containing RPC (equivalent of 1 g of choline chloride [CC]), no RPC (negative cont ...

Choline is a methyl donor, which may influence DNA methylation, gene expression, and cellular processes. Past studies have found an increase in milk yield when periparturient dairy cows were supplemented with dietary rumen-protected choline (RPC); however, the mechanism behind this response is unknown. Therefore, the objective of this study was to assess the effects of dietary RPC supplementation ...