Thermostability of enzyme during pelleting is always a big question. It is very difficult to asses the effect of enzymes. Some enzyme producers claim it is intrinsically thermostable coated and so on. In true sense, post pelleting application is right method of enzyme application although we require sophisticated applicators. But still the efficacy of enzyme varies with the composition of diet, substrate present, enzyme concentration and favorable conditions like pH and temperature. Instead, we should develop a technology for in vitro application of enzymes to different feed ingredients based on their substrate analysis. This will be more economical and will serve the purpose of enzyme application to some extent although it looks to be more challenging today.