The objective of this study is to evaluate the effects that supplementing cattle with C18 fatty acids of differing saturation can have on rumen tissue fatty acid composition and reticulo-rumen short chain fatty acid absorption. Eight heifers were used in a duplicate 4 × 4 Latin Square Design. Heifers were supplemented with fatty acids at a fixed rate of 0.06% of BW. Treatments included control (CON; no supplemental fatty acid), saturated (SAT; supplemental stearic acid, 70% C18:0), unsaturated (UNSAT; supplemental linoleic acid, 74% C18:2, 36% conjugated linoleic acid), and control isocaloric treatment (CON-ISO; no supplemental fatty acid, but isocaloric to both SAT and UNSAT). Total-tract permeability was measured using Cr-EDTA and short chain fatty acid absorption across the reticulo-rumen was measured by the temporarily isolated and washed reticulo-rumen technique. Rumen tissue biopsies and blood samples were collected at the beginning and end of each period. Rumen tissue will be measured for fatty acid composition and blood will be analyzed for fatty acid composition, non-esterified fatty acids, beta-hydroxy butyrate, and cholesterol.
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