Egg yolk antibodies (IgY) against Bovine Leukemia Virus
Published: October 18, 2018
By: Cecilia Martínez, Gerónimo Gutiérrez, Irene Alvarez, Natalia Porta, Marina Lomónaco, Andrés Wigdorovitz, Pablo Chacana, Karina Trono. / Instituto de Virologia, INTA, Castelar, Argentina.
Summary
Bovine Leukemia Virus (BLV) is distributed worldwide and causes important economic losses on dairy farms. Currently, there are no effective vaccines or antivirals against BLV. Egg yolk antibodies (IgY) has many advantages over mammalian IgG. Despite the higher yields, they are non-invasively extracted from egg yolk, do not cross react against mammalian antigens or activate the mammalian complement system. In this work, we evaluate the reactivity of Igy antibodies against Bovine Leukemia Virus p24 core protein and against the whole virus particle. Hens were immunized by intramuscular inoculation with purified p24 or the virus particle until the development of high antibody-titers. Total IgY was purified from egg yolks by ammonium sulfate precipitation. The purified egg yolk antibodies strongly reacted with BLV particles from a persistently infected cell line, with supernatants from ex vivo cultures of PBMCs from natural infected animals and also with purified p24 by both ELISA and Western blot. These data suggest that chicken IgY may be a suitable platform to produce large amounts of anti-BLV antibodies for diagnostic systems. Furthermore, the use of IgY for passive immunization against BLV infection should also be explored in order to develop new strategies to control the disease in cattle.
From 16th International Conference on Human Retroviruses: HTLV and Related Viruses Montreal, Canada. 26-30 June 2013
This article was originally published in Retrovirology 2014, 11(Suppl 1):P46. http://www.retrovirology.com/content/11/S1/P46. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0).
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13 de enero de 2019
Although production of IgY by hyper-immunizing laying bird and harvesting from eggs is an easy process there are few issues: (a) administering intravenous is not without problem due to immunological response and (b) when given orally these are very sensitive to proteolytic enzymes. There are reports to suggest that major part of IgY is destroyed in intestine.
Prof. (Dr) Abdul Samad
Ph.D. (OVC, Guelph)
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13 de enero de 2019
Good to hear about this disease related to dairy cattle, but my concerns is that, what dairy breed is very sensitive against this disease? Since nowadays the live animal business from dairy developing country to non-developed country is seriously running, but there are so many problems in imported or receiver country, especially in terms of feeding and housing, as well as proper selection of the cows during ordering process. Thanks.
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9 de enero de 2019
It’s a very interesting article, BLV is very important disease big challenge in Dairy cattle’s industry their accurate detection for the virus by using iiPCR high technology instruments used on the dairy filed DNA/RNA technology Rapid PCR.
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