Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses

Published on: 03/02/2017
Author/s : Magdalena Escorcia 1, Karol Carrillo-Sánchez 2, Santiago March-Mifsut 2, Joaquin Chapa 3, Eduardo Lucio 3, Gerardo M. Nava 4. / 1 Departamento de Producción Animal Aves. Facultad de Medicina Veterinaria y Zootecnia. Universidad Nacional Autónoma de México, Coyoacán, D.F., México; 2 Instituto Nacional de Medicina Genómica, Álvaro Obregón, D.F., México; 3 Investigación Aplicada, Tehuacán, Puebla, México; 4 Washington University School of Medicine. Dept. Pathology and Immunology, St. Louis, MO, USA.

  Background Avian Influenza (AI) virus belongs to the Orthomyxoviridae family, Influenzavirus A genus. This virus possesses eight segments of single-stranded RNA genome. Two of these segments encode for two important membrane glycoproteins, hemagglutinin (HA) and neuraminidase (NA) [1], that play a key role during cellular infection. These two proteins are used for virus subtype classifica...

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Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
02/03/2017 |

Very interesting research work. I agree with the authors and it is now the turn for other researchers to confirm the drift in other AI viruses. Previously we were accustomed to the Highly Pathogenic H5N1 virus. Nowadays, we find various different strains of Highly Pathogenic AI virus. In 2016 & 2017, many European and Asian countries had reported cases of AI in both poultry and wild birds. One author had raised a point concerning no outbreak of AI in South America.

Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
02/03/2017 | Good morning Dr, I appreciate your comments. At the moment we are developing the project to analyze the viruses that have been reported to the OIE and whose sequence is available in the information banks. The results could give us a lot of information regarding what is happening.
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
02/03/2017 | In relation to the comment made on the presence of AI in South America, the information given by the Brazilian authorities in a presentation that I had in that country, was that the migratory routes of the Anatides did not reach so far south of the continent.
Dr. Jignesh Barot
M. V. Sc. (Avian Diseases)
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
07/03/2017 | Congratulations!!!
Really very good research.

"The HI assay demonstrated highly variable results when field LPAI H5N2 viruses where tested against antisera derived from year-specific AI H5N2 strains.

Could you please explain what variation was observed in HI test (differences in HI titers - log2n)?

Thanks
Dr. Jignesh Barot
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
07/03/2017 | Thank you for your congratulations. Your question is very good! Dr. Jignesh Barot

When the AI virus is antigenically disassociated even though it is the same subtype, the standard antisera no longer adequately recognize the virus that presents antigenic variation and that causes the difference reported with the HI technique.
Dr. Jignesh Barot
M. V. Sc. (Avian Diseases)
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
07/03/2017 | Thanks for reply
Does it mean that there will not be inhibition of haemagglutination?
Panya Boonprasert
Agriculturist
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
08/03/2017 | That very interesting and we can get more knowledge by this matter.
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
08/03/2017 | Thank you, your comments encourage us to follow our line of research.
Re: Impact of antigenic and genetic drift on the serologic surveillance of H5N2 avian influenza viruses
08/03/2017 | Thank you for your question Dr Jignesh Barot. According to what we have observed, as the virus distances itself in relation to its ancestor, due to the antigenic changes that it suffers, the recognition of the standard antisera towards the virus is no longer effective and the test gives us false Negative results. Hence the importance of updating the antisera.
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