Dear Users,
This is an interesting forum from our Spanish community, generated by Ester Gómez from Spain:
We are a research group that seeks to develop a methodology for AFM1 by HPLC analysis, previous extraction in immune-columns, based on the ISO14501 guidelines and AOAC. Despite being a widely studied topic we found the following practical problems:
- In certain solvents or mixture of these, eg:. methanol, standard AFM1 presents some additional peaks found when the pattern is dissolved in acetonitrile 10 v / v (recommendation of the above standard ISO). Is there a reference or studies about the degradation of the analyte as the solvent?
- When following the protocol described for a type determined immunoaffinity column, HPLC analysis revealed retention times than those obtained for ACN10 pattern, matching with those additional peaks. This would represent evidence of degradation AFM1 in the effluent from the column? How could it be solved?
Ester Gómez
I have been using VICAM immunoaffinity column and we get better results on both VICAM reader and HPLC by uising methanol as solvent and mobile phase. check if you can use methanol.
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