The study was conducted to evaluate the effect of herbs as feed additive on performance of broiler alternative to antibiotics. One-day-old (N=320) broiler chicks were divided into eight treatment groups with four replicates of ten chicks. Six treatments were three herbs i.e. Allium sativum, Cassia angustifolia and Artemisia scoparia @ 0.5 and 1.0% respectively, control (basal diet), and a positive control (Oxyfeed® @ 2g/kg). In the study, the treatment groups, which were given diet supplemented with herb and antibiotic, exhibited improved weight gain, average daily gain, and feed conversion (P<0.05). No significant difference was observed in feed intake (P>0.05). The treatment groups supplemented with Allium sativum showed higher growth performance compared to Cassia angustifolia and Artemisia scoparia and control (P<0.05). Relative organ weights and relative carcass yield of treatment groups were not significantly different (P>0.05). The Relative length of the intestine of the treatment groups showed significant difference (P<0.05). Supplementation of herbal additives and antibiotic had a positive effect on bacterial enumeration of Ileum (P<0.05). The use of antibiotic caused a reduction in all three forms of microbial population. On the other hand, herbs added as feed additive acted as prebiotic and enhanced the lactic acid bacteria. In conclusion, supplementation of herbs as feed additive improved growth parameters and present results suggests herbs could be used as alternative to antibiotics growth promoting feed additives.
Aarestrup, F.M., Bager, F. and Andersen, J.S., 2000. Association between the use of avilamycin for growth promotion and the occurrence of resistance among Enterococcus faecium from broilers: epidemiological study and changes over time. Microb. Drug Resist., 6: 71-75. https://doi. org/10.1089/mdr.2000.6.71
Aarestrup, F.M., Seyfarth, A.M., Emborg, H.D., Pedersen, K., Hendriksen, R.S. and Bager, F., 2001. Effect of abolishment of the use of antimicrobial agents for growth promotion on occurrence of antimicrobial resistance in fecal enterococci from food animals in Denmark. Antimicrob. Agents Chemother., 45: 2054-2059. https://doi. org/10.1128/AAC.45.7.2054-2059.2001
Alam, N. and Gupta, P.C., 1986. Structure of a watersoluble polysaccharide from the seeds of Cassia angustifolia. Pl. Med., 4: 308-310. https://doi. org/10.1055/s-2007-969161
Amagase, H., 2006. Clarifying the real bioactive constituents of garlic. J. Nutr., 136: 716S-725S.
Andleeb, S., Tahir, M., Khalid, M., Awan, U. A., Riaz, N. and Ali, S., 2014. Antibacterial and antioxidant activities of traditional herbs and honey against fish associated bacterial pathogens. Pakistan J. Zool, 46: 933-940.
Annison, G. and Choct, M., 1991. Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies minimizing their effects. World Poult. Sci. J., 47: 232-242. https://doi. org/10.1079/WPS19910019
Boling, S.D. and Firman, J.D., 1998. Digestible lysine requirement of female turkeys during the starter period. Poult. Sci., 77: 547-551. https://doi. org/10.1093/ps/77.4.547
Cabuk, M., Bozkurt, M., Alcicek, A., Akbas, Y. and Küçükyilmaz, K., 2006. Effect of an herbal essential oil mixture on growth and internal organ weight of broilers from young and old breeder flocks. S. Afri. J. Anim. Sci., 36: 135-141. https://doi.org/10.4314/ sajas.v36i2.3996
Casewell, M., Friis, C., Marco, E., McMullin, P. and Phillips, I., 2003. The European ban on growthpromoting antibiotics and emerging consequences for human and animal health. J. Antimicrob. Chemother., 52: 159-161. https://doi.org/10.1093/ jac/dkg313
Castanon, J.I.R., 2007. History of the use of antibiotic as growth promoters in European poultry feeds. Poult. Sci., 86: 2466-2471. https://doi.org/10.3382/ps.2007-00249
Choi, I.H., Park, W.Y. and Kim, Y.J., 2010. Effects of dietary garlic powder and α-tocopherol supplementation on performance, serum cholesterol level, and meat quality of chicken. Poult. Sci., 89: 1724-1731. https://doi.org/10.3382/ps.2009-00052
Chrubasik, S., Pittler, M.H. and Roufogalis, B.D., 2005. Zingiberisrhizoma: a comprehensive review on the ginger effect and efficacy profiles. Phytomedian, 12: 684-701. https://doi.org/10.1016/j. phymed.2004.07.009
Dahiya, N., Tewari, R. and Hoondal, G.S., 2006. Biotechnological aspects of chitinolytic enzymes: a review. Appl. Microb. Biotech., 71: 773-782. https://doi.org/10.1007/s00253-005-0183-7
Dibner, J.J. and Richards, J.D., 2005. Antibiotic growth promoters in agriculture: history and mode of action. Poult. Sci., 84: 634-643. https://doi. org/10.1093/ps/84.4.634
Dorhoi, A., Dobrean, V., Zahan, M. and Virag, P., 2006. Modulatory effects of several herbal extracts on avian peripheral blood cell immune responses. Phytother. Res., 20: 352-358. https://doi. org/10.1002/ptr.1859
Elagib, H.A., El-Amin, W.I.A. and Malik, H.E.E., 2013. Effect of dietary garlic (Allium sativum) supplementation as feed additive on broiler performance and blood profile. J. Anim. Sci. Adv., 3: 58-64. https://doi.org/10.5455/ jasa.20130219104029
El-Latif, A.S.A., Saleh, N.S., Allam, T.S. and Ghazy, E.W., 2013. The effects of rosemary (Rosemarinus officinalis) and garlic (Allium sativum) essential oils on performance, hematological, biochemical and immunological parameters of broiler chickens. Br. J. Poult. Sci., 2: 16-24.
Engberg, R.M., Hedemann, M.S., Leser, T.D. and Jensen, B.B., 2000. Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broiler. Poult. Sci., 79: 1311. https://doi.org/10.1093/ps/79.9.1311
FAO, 2003. OIE/WHO Expert workshop on non-human antimicrobial usage and antimicrobial resistance: First Workshop on Scientific Assessment, 1–5 December 2003, Geneva, World Health Organization.
Gilani, A.U.H. and Janbaz, K.H., 1993. Protective effect of Artemisia scoparia extract against acetaminophen-induced hepatotoxicity. Gen. Pharmacol. Vascu. Syst., 24: 1455-1458. https:// doi.org/10.1016/0306-3623(93)90434-Y
Hardy, H., Harris, J., Lyon, E., Beal, J. and Foey, A.D., 2013. Probiotics, prebiotics and immunomodulation of gut mucosal defences: homeostasis and immune pathology. Nutrients, 5: 1869-1912. https://doi. org/10.3390/nu5061869
Jamroz, D., Orda, J., Kamel, C., Wiliczkiewicz, A., Wertelecki, T. and Skorupinska, J., 2003. The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics, and gut microbial status in broiler chickens. J. Anim. Feed Sci., 12: 583-596. https://doi.org/10.22358/ jafs/67752/2003
Kabir, S.M., 2009. The role of probiotics in the poultry industry. Int. J. mol. Sci., 10: 3531-3546. https:// doi.org/10.3390/ijms10083531
Lu, L. and Walker, W.A., 2001. Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. Am. J. clin. Nutr., 73: 1124S-1130S.
Lukanov, H., Genchev, A. and Ribarski, S., 2015. Effect of feed supplementation with garlic power on meat productivity and meat quality traits of classic ross 308 male hybrid chickens. Trak. J. Sci., 13: 67. https://doi.org/10.15547/tjs.2015.01.009
Mathew, A.G., Cissell, R. and Liamthong, S., 2007. Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production. Fd. Borne Path. Dis., 4: 115- 133. https://doi.org/10.1089/fpd.2006.0066
McReynolds, J., Waneck, C., Byrd, J., Genovese, K., Duke, S. and Nisbet, D., 2009. Efficacy of multistrain direct-fed microbial and phytogenetic products in reducing necrotic enteritis in commercial broilers. Poult. Sci., 88: 2075-2080. https://doi.org/10.3382/ ps.2009-00106
Negahban, M., Moharramipour, S. and Sefidkon, F., 2006. Chemical composition and insecticidal activity of Artemisia scoparia essential oil against three coleopteran stored-product insects. J. AsiaPacific Ent., 9: 381-388. https://doi.org/10.1016/ S1226-8615(08)60276-9
Niewold, T.A., 2007. The non-antibiotic antiinflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poult. Sci., 86: 605-609. https://doi.org/10.1093/ ps/86.4.605
Ramiah, S.K., Zulkifli, I., Rahim, N.A.A., Ebrahimi, M. and Meng, G.Y., 2014. Effects of two herbal extracts and virginiamycin supplementation on growth performance, intestinal microflora population and fatty acid composition in broiler chickens. AsianAust. J. Anim. Sci., 27: 375. https://doi.org/10.5713/ ajas.2013.13030
Shin, L., Pelley, R.P. and Kripke, M.L., 1995. Use of Aloe vera yoghurt for acid production, viscosity, sensory properties and quality retention. J. Invest. Derm. 102: 197-204.
Singh, H.P., Mittal, S., Kaur, S., Batish, D.R. and Kohli, R.K., 2009. Chemical composition and antioxidant activity of essential oil from residues of Artemisia scoparia. Fd. Chem., 114: 642-645. https://doi. org/10.1016/j.foodchem.2008.09.101
Sultana, S., Ahmad, M., Zafar, M., Khan, M.A. and Arshad, M., 2012. Authentication of herbal drug Senna (Cassia angustifolia Vahl.): A village pharmacy for Indo-Pak subcontinent. Afri. J. Pharm. Pharmacol., 6: 2299-2308. https://doi. org/10.5897/ajpp12.446
Tareen, R.B., Bibi, T., Khan, M.A., Ahmad, M. and Zafar, M., 2010. Indigenous knowledge of folk medicine by the women of Kalat and Khuzdar regions of Balochistan, Pakistan. Pak. J. Bot., 42: 1465-1485.
Wati, T., Ghosh, T.K., Syed, B. and Haldar, S., 2015. Comparative efficacy of a phytogenic feed additive and an antibiotic growth promoter on production performance, caecal microbial population and humoral immune response of broiler chickens inoculated with enteric pathogens. Anim. Nutr., 1: 213-219. https://doi.org/10.1016/j. aninu.2015.08.003
Wang, Z.R., Qiao, S.Y., Lu, W.Q. and Li, D.F., 2005. Effects of enzyme supplementation on performance, nutrient digestibility, gastrointestinal morphology, and volatile fatty acid profiles in the hindgut of broilers fed wheat-based diets. Poult. Sci., 84: 875- 881. https://doi.org/10.1093/ps/84.6.875
Wenk, C., 2003. Herbs and botanicals as feed additives in monogastric animals. Asian Aust. J. Anim. Sci., 16: 282-289. https://doi.org/10.5713/ajas.2003.282
Zeybek, U., Yaylim, I., Yilmaz, H., Agaçhan, B., Ergen, A., Arikan, S., Bayrak, S. and Isbir, T., 2007. Methylene tetra hydro folate reductase C677T polymorphism in patients with gastric and colorectal cancer. Cell Biochem. Funct., 25: 419- 422. https://doi.org/10.1002/cbf.1317