How to conduct protein expression in bacteria? I searched the internet, and learned that The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and s

Question

How to conduct protein expression in bacteria? I searched the internet, and learned that The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins. (source: http://www.creative-biolabs.com/bacterial-expression-systems-e-coli-bacillus.html)

Can anyone show me more? Thanks!

Adrian Calderon · Accepted Answer

Saludo cordial al Doctor Cadú, y todos los foreros que me puedan aconsejar..
Es un gusto formar parte de esta gran comunidad que es Engormix.com, Somos Adrián y Patricia de Costa Rica, estamos interesados en impletar esta excelente técnica. 
Tenemos muy buenos números comprarados a la técnica que hoy empleamos y aún tenemos algunas dudas 

1. Cuál es tamaño optimo del hato a manejar por módulo.
2. Un hato de 18 a 22 animales por modulo de 4.5 Has dividido en 30 apartos de 1500 m2 cada uno será bueno, mi correo es acero332001@gmail.com

 Mucha gracias

How to conduct protein expression in bacteria? I searched the internet, and learned that The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and s

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