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Blood heat shock proteins evoked by some Salmonella strains infection in ducks

Published on: 5/17/2022
Author/s : Kamelia Osman 1, Ihab Ibrahim 2, Ashgan Yousef 3, Tanios Nabil 4 & AlAtfeehy Nayerah 4 / 1 Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt; 2 Center of Excellence in Biotechnology, King Saud University, Riyadh, Kingdom of Saudi Arabia; 3 College of Applied Studies and Community Services, King Saud University, Riyadh, Kingdom of Saudi Arabia; 4 Department of Poultry Diseases, Animal Research Institute, Dokki, Egypt.
Summary

Bacterial heat-shock response is a global regulatory system required for effective adaptation to changes (stress) in the environment. An in vitro study was conducted to investigate the impact of a sublethal temperature (42°C) on heat shock protein (HSP) expression in 6 Salmonella strains (Salmonella Enteritidis, S. Typhimurium, S. Virchow, S. Shubra, S. Haifa and S. Eingedi). The 6 Salmonella strains were isolated from the tissues of ducklings that had died from avian salmonellosis. To determine the induction of HSP in the 6 Salmonella strains, they were exposed to the selected temperature level for 24 h and further kept for 48 h at culturing condition of 42°C. Growth under a sublethal temperature of 42°C increased the expression of several proteins of Salmonella, including a 63 kDa protein in addition to the generation and/or overexpression of 143 proteins which were specific to heat shock, concurrent to this acquired thermotolerance. The 6 Salmonella strains responded to 24 h of thermal stress at an elevated temperature 42°C by synthesizing different heat shock proteins (HSP) with molecular weights ranging between 13.62 and 96.61 kDa. At 48 h, the 6 Salmonella strains synthesized different HSPs with molecular weights ranging between 14.53 and 103.43 kDa. It follows that salmonellae would produce HSPs during the course of the infectious process. Salmonellosis produced several proteins after 24 and 48 h of infection. Seven of these proteins (100, 80, 60, 40, 30, 20 and 10 kDa) were recognized in the serum obtained from the ducklings infected with S. Enteritidis, S. Typhimurium, S. Virchow, S. Shubra, S. Haifa and S. Eingedi after 24 h of infection. After 48 h, the 1–7 kDa HSP became more evident and indicated their de novo generation.

    

Abstract published in World J Microbiol Biotechnol 28, 1997–2001 (2012). https://doi.org/10.1007/s11274-012-1002-9.

 
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