Dr. Andrew Van Kessel
I congratulate the work team for the study conducted. The results obtained seem to me to be consistent with those of the current literature. I would like to highlight, among other details, some of the evaluated parameters, such as; genetic expression of gut health markers and immune response, concentration of antioxidants in the serum, as well as the concentrations of ammonia, SCFA and BCFA in the cecum and colon digestes, depending on the levels of fiber and threonine in the diets, and their possible interactions. One detail that caught my attention was the fact that the high protein diet not only decreased the requirement for threonine to maximize protein deposition, but also increased the amount deposited. As experimental diets are isolisinic, this lower protein deposition, in diets with lower levels of protein, regardless of fiber level, could not be associated with a worse in the relationship of essential amino acids to non-essential.