Survivability of Porcine Epidemic Diarrhea Virus in Slurry

Porcine Epidemic Diarrhea (PED) virus is a major enteric swine disease of economic significant in many Asia country and PED was first recognized in late April of 2013 in USA.

According to some research, PED virus survived in manure slurry for 28 days at 4℃ and possible longer.

The main purpose of this study is to investigate the time to inactivate of the PED virus in manure slurry on farm.

Experimental farm : This farm has 4barns with 1600 pigs as finishing site. PED has occurred on January 23, 2014, and all pigs were removed after 7 days from this farm.

PED virus detection : which have been posted depopulation on the day 41 to 161, to verify the presence of PED virus. The samples were tested via PED virus PCR.

Inoculation in piglets : The samples which post depopulation, day82 and day107, were passed into ped virus naïve to 5days old piglets via oral-gastric tube. These pigs served as a bioassay to detect the presence of infectious PED virus. Fecal swabs were collected every 24 hours. Swabs were tested via PED virus PCR. Necropsy & IHC (Immunohistochemistry) for small intestine was performed on 48 hours and 72 hours.

PED virus PCR test of slurry was positive until 161days post depopulation.

Inoculated piglet via slurry at 82 days(a) post depopulation was infected with PED virus. But inoculated piglet via slurry at 107 days(b) post depopulation was not infected with PED virus.

PED virus can present in slurry during 161 days post depopulation. But It doesn`t mean infectious virus.

And PED virus in slurry has possibility to infect pigs during 82 days since post depopulation.

These results suggest that PED virus in slurry could be longer has infectivity than we know. Therefore, management strategy of slurry is necessary to control PED virus in the farm.

Disclosure of Interest: None Declared.