Screening of Seventeen Asian Medicinal Plants for Antiviral Activity against Field PRRSV Strain in Taiwan
Published: December 5, 2024
By: K. Kaewprom 1, 2, C.-N. Lin 2, 3, M.-T. Chiou 2, 3 / 1 Program of Veterinary Technology, Faculty of Agricultural Technology, Rajabhat Mahasarakham University, Mahasarakham, Thailand; 2 Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology; 3 Veterinary Medicine, Animal Disease Diagnostic
Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan, Province of China.
Summary
Keywords: Asian herb extracts, field PRRSV strain, qPCR
Introduction:
Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure of sows and respiratory problems of nursery and growing pigs. Present management strategies mainly focus on the prevention of infection using vaccination but are not sufficient to eradicate the virus and provide complete immunity. Therefore, in searching for agents that may prove clinically effective against PRRSV infection, seventeen Asian medicinal plant extracts were screened for their antiviral activity against field PRRSV strain in MARC-145 cells.
Materials and Methods:
An immunofluorescence assay (IFA) was detected viral replication, MTT assay was employed for analysis of cytotoxicity test and quantitative polymerase chain reaction (qPCR) were conducted to detected the effect of these extract on viral loads.
Results:
Based on the results obtained from the cell viability tests, the highest concentration showing less than 10% cytotoxicity in MARC-145 cells was chosen for each for the plant extracts. The antiviral activity assay indicated that Nepeta cataria, Cinnamomum cassia and Perilla fructescence extracts had a significant reducing the PRRSV load in vitro.
Conclusion:
In the future, we will be investigating further the antiviral mechanisms of this plant extract on PRRSV infection and applying to clinical trials on a swine farm.
Disclosure of Interest: None Declared.
Published in the proceedings of the International Pig Veterinary Society Congress – IPVS2016. For information on the event, past and future editions, check out https://www.theipvs.com/future-congresses/.
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