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Amino Acid Digestibility and Energy Utilization of a High Corn-DDGS Diet Supplemented with a Carbohydrase Mixture

Published: November 30, 2012
By: Lee H, Ramirez E, Price KL, Jeffery Escobar(Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA.)
Introduction
The non-starch polysaccharide content of corn-derived distiller dried gains with soluble (DDGS) is 3-4 times higher compared to corn. Some of the anti-nutritional effects of its high fiber content may be ameliorated with the supplementation of carbohydrases. Our objective was to measure in pigs increases in amino acid (AA) digestibility and energy utilization in a diet with high inclusion of DDGS and supplemented with a carbohydrase mixture.
Materials and Methods
Eight crossbred pigs (47.0 ± 1.5 kg) were surgically fitted with a simple T cannula at the distal ileum (Wubben et al., 2001). Apparent and standardized (SID) AA digestibility and ENL (Stein et al., 2007), and apparent metabolizable energy (aME) and apparent energy metabolizability (AEM, Adeola et al., 2011) were determined. Treatments were corn-soybean meal-based or 40% DDGS-based diet supplemented or not with a carbohydrase mixture. A 7-d washout period was used between sample collection periods. Data were analyzed as a replicated 4x4 Latin Square Design using proc mixed (SAS® Institute, Gary, NC).
Results and Discussion
Carbohydrase supplementation improved the SID of Arg, Ile, Met, Phe, Thr, Ala, Glx, Ser, and Tyr (P < 0.04), while Leu, Lys, Val, and Gly tended (P < 0.10) to increase. Pigs given DDGS-based diet had lower SID for all measured AA (P < 0.04) except Leu, Met, Ala, Glx, and Tyr. Carbohydrase supplementation had no effect (P= 0.48 to 0.85) on aME or AEM. Compared to corn-soybean meal diet, inclusion of DDGS increased urinary energy excretion and carbohydrase supplementation tended to reduce it (P < 0.09).
Conclusions
Carbohydrase supplementation increased AA digestibility but had no effect on energy utilization in growing pigs.
Implications
Carbohydrase supplementation may be used to increase AA digestion in diets containing high inclusion levels of DDGS or other high-fiber containing feedstuffs.
References
Adeola L et al. 2011. J. Anim. Sci. 89:3189-3218; Stein H et al., 2007. J. Anim. Sci. 85:172-180; Wubben J et al., 2001. Contemp. Top. Lab. Anim. Sci. 40:27-31. Keywords: Amino acid, digestibility, metabolizable energy, carbohydrase.
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Jeffery Escobar
Mazen Animal Health
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Rafael Duran
IFF - International Flavors & Fragrances
25 de julio de 2013
Hello Jeffery, interesting findings you came across with but I am missing - I cannot find the whole of the paper - which activities you included in that cocktail and why you decided those activities and not others. Since corn-DDGS at 40% would increase the NSP/DF content in the diet (dilute), did you increase the fat/oil level in the tested diet?; if so, could this not have an influence in the final findings?. How would you explain that your cocktail only improved sign. the AA digestibilities but not the ME?. Many thanks for your answer.
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