Efficacy of toxibond use in swine diets in the growing stage, as a toxin adsorbent in the presence of aflatoxin b1 (ab1) + ochratoxin

A trial was performed with castrated male hogs of the Belgian Landrace x Belgian Landrace, in the finishing stage, from 23 Kg. of weight to 90 Kg., during the 101 days of the test; feed consumed was based on corn-soybean with an average content of 5 ppm aflatoxin B1 (AB1) and 50 ppb of Ochratoxin; a parallel trial was carried with added TOXIBOND as a sequestering agent, and; a third one, with the mycotoxins in question absent was verified in the laboratory.

20 animals per treatment were used in the trial, distributed into groups of 10 animals with two repetitions each for a total of 60 animals; measurement of data was performed from the first day of ration consumption and then every 7 days.

The treatments were: Treatment 1 or control, where a diet without added mycotoxins under study was offered, verified by analysis at the Colombian National University; Treatment 2, where the same diet was provided, but contaminated with 5 ppm. Aflatoxin B1+ 50 ppb. Ochratoxin, without adding the adsorbent TOXIBOND and Treatment 3, in which the same contaminated diet was used adding TOXIBOND at a rate of 2.5 Kg. per ton of feed.

In the three cases an isoproteic, isoenergetic, isovitaminic and mineral diet based on corn-soy was provided in compliance with NRC requirements and in which the absence of Aflatoxins and Ochratoxins was verified in the laboratory; treatments 2 and 3 were contaminated with Aflatoxin B1 and with Ochratoxin.

In the statistical analysis of the trial we employed a linear trend statistical design to observe the additive’s effect on weight gain and final weight of the hogs in the presence or absence of the adsorbent in the diet, adjusted to a fully randomized design.

EFFECT OF THE USE OF TOXIBOND ON SWINE CONSUMING DIETS CONTAMINATED WITH AFB1 + OCHRATOXIN

Efficacy of toxibond use in swine diets in the growing stage, as a toxin adsorbent in the presence of aflatoxin b1 (ab1) + ochratoxin - Image 1

Mycotoxins used were from SIGMA Laboratories in the USA; the determination of mycotoxins was performed through Neogen Laboratories’ quantitative ELISA method and through Thin Film Chromatography at the Colombian National University's mycotoxins laboratory.

The results obtained at the end of the experiment, after a 101 day trial, show how treatment 1 (toxin-free- TOXIBOND-free) shows better results than the other two; however, we can observe that, although treatment 3 (toxins+TOXIBOND) exhibited lower performance than T1, it was not significantly lower despite the presence of the toxins AFB1+OCR, which have, according to the literature, a negative synergistic effect on all production parameters.

Treatment 2 (with toxins- TOXIBOND-free) exhibited worse results; mortality was seen, lesions were found upon necropsy at the level of liver, kidneys, muscles and intestinal mucosa.

Treatments 1 and 3 did not exhibit mortality nor were lesions observed upon necropsy.

In T2, we observed the worst feed conversion and the worst weight gain, in addition to mortality, for which reason at the trial’s end, 254 Kg. less meat than in T1 and 227 Kg. less than in T3 were produced; figures that are economically very significant.

It is important to highlight the palliative effect exercised by TOXIBOND on the synergistic action of the mycotoxins Alfatoxin B1 and Ochratoxin.

In the course of the trial, animals in T3 were observed to be calm and with good appetites, a situation not seen in T2 where the animals were always agitated.