This study was conducted to assess the effect of 17α- methyl-testosterone, wet testes of boar (Sus. domesticus) and pawpaw seed powder based diet (Carica papaya) on masculinization of Nile tilapia fry in an indoor experimental ponds. The efficacies in producing monosex males were determined after 4months. Fifty five fry were assigned randomly to five experimental ponds in triplicate. The feed composed of soya-bean, fish meal, wheat bran, cassava flour in Pond A1, A2,A3, A4 and A5 were formulated by adding 0.06g of 17α- methyl-testosterone (A1) 17.47g of bull wet testes (A2) 25.59g of boar wet testes (A3), 20.48g of bull/African mud catfish (A4) at (80:20), and (A5) 20g of pawpaw seed powder respectively per kg of feed. Results from the morphological examination revealed significant different (p<0.05) in phenotypic males of Nile tilapia fry fed with the highest percent in MT-treated diet (A1) of 100±0.00a while A2 and A3 had males of 93.40±0.76b 78.48±2.40d respectively. The phytochemical constituent found in the experimental pawpaw seed powder showed that it contains Cyanogenic glycoside of 0.042% which was the lowest constituent followed by Tannin 9.23% having the highest constituent. Therefore, natural product can be an alternative method to produce all-male tilapia population in an environment-friendly manner
Keywords: Oreochromis niloticus, monosex, 17αmethyl-testosterone, organic farming.
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