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Amino Acids: Looking beyond Protein Levels for Animal Performance

Published: July 21, 2017
By: Dave Albin, Insta-pro International
In this industry, we talk about protein a lot. This is because it helps evaluate the value of an ingredient or diet. The type of protein we are talking about though is “crude protein”, which is a routine chemical measure that’s been around since the 1800’s.
Amino Acids: Looking beyond Protein Levels for Animal Performance - Image 1
Of course, it’s been refined since then, but the chemical reaction is basically the same. Other predictive methods (LECO and NIR) have been based on results from the Kjeldahl method – the original chemical method named after the developer.
So, you’ll often hear us say that a soy meal has a certain protein level, or a complete fish diet has been formulated to contain a certain protein percentage, and so on.
This is all appropriate to do, but sometimes this creates confusion because animals and humans don’t need protein – it is the amino acids that make up proteins which are actually required. Without adequate amounts of these amino acids, it would not be possible to maintain muscle mass, create hormones that perform numerous functions, secrete enzymes that allow digestion, or repair skin, just to name a few.
Crude protein uses the term “crude” on purpose because this value reflects not only amino acids, but also anything with organic nitrogen. The amount of nitrogen is then used to reflect the amount of amino acids present. You can see the drawbacks right away – some of the nitrogen almost always comes from sources other than protein-building amino acids. In addition, two materials can have the same crude protein level, but have very different levels of individual amino acids.
So, to fully evaluate the value of an ingredient or diet, we have to get to the level of amino acids.
When formulating a diet, this often means not allowing yourself to get caught up with a particular protein percentage that you or your formulation program wants you to meet. I’ve formulated numerous diets over the years where I was able to target a lower percentage of crude protein by using higher-quality ingredients, such as extruded full-fat soy and extruded/pressed (ExPress®) meal, as well as synthetic amino acids, such as L-lysine and DL-methionine, to meet the nutrient requirements of the animal.
This approach is often cheaper because the protein ingredients, typically most expensive per unit mass, can be used at lower levels. In addition, as I’ve documented here before, excess protein cannot be stored, it has to be excreted, and this comes at an energy cost to the animal.
So, in conclusion, while it’s still important to understand the “protein” level of ingredients and diets for the numerous beneficial reasons outlined here, it’s important to look deeper to the amino acid level and understand the true value of what you’re working with. Please send us any questions about this topic, and we will work with you to optimize your operation.
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Luis Mansilla
13 de octubre de 2017

In relation to the opinion expressed by the member that preceded me, it is propitious to clarify that in Peru, country in process of a solid industrialization in South America; and with the largest biodiversity on the planet (sum of the variety of flora, fauna and marine biomass), if the importance of the Aa is fully understood. in its entire context, where, at the level of the analytical tests carried out by prestigious companies based in Peru, it is clearly understood that the analyzes are clearly directed to the characterization and calculation of protein concentration, to fix the coefficient of extinction (EC), determination of total Aa, without exclusion of the triad: cysteine, tryptophan and hydroxyproline; as well as free Aa, where asparagine and glutamine are considered. I conclude by mentioning that in the neighbors of the hemisphere, vaguely called Third World, they also have clear discernment and focus on the Aa. Greetings.

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Luis Mansilla
4 de septiembre de 2017

It is unquestionable that the level of crude protein and/or digestible protein is not sufficient according to the requirement of the mono or polygastric species. In fact, within the essentiality of Aa, whose concept is relative by the interaction between them and other molecules, the technical article commits us to deal with the term; Assertive Management of Aa, so we can not fail to take into account Aa hepatoprotectors, because the liver is an essential axis within the animal metabolism and because it is linked in the animal immune system. In this context, eg. the carnitine, to which I will make particular reference, is an amino acid synthesized from the lysine and methionine, mainly at the level of the liver and kidneys. being a very important lipotropic factor.

L-carnitine has indispensable functions in the intermediate metabolism and is received by endogenous synthesis and exogenous sources. It plays a role in the metabolism of fatty acids by directing them into the mitochondrial oxidative pathway through the action of specialized acyltransferases. In poultry production, L-carnitine has been shown to have a multifunctional purpose, which includes: promotion of growth, strengthening of the immune system, antioxidant effects and enhancement of broiler semen quality. In another study, L- supplemental carnitine increased body weight gain (BWG) and improved feed conversion (HR) during the first 2 weeks of study. FC was also improved during the fourth week of the experiment.

In a study in pigs, it was concluded that the stimulating effect of carnitine on the growth of the litters may be due to a better lactation behavior of the piglets born of L-carnitine, which causes that the milk production of the bristles increases. That is, dietary supplementation of l-carnitine increases the reproductive performance of the bristles. These findings suggest that endogenous L-carnitine synthesis is insufficient to meet the metabolic requirement of sows during gestation. Finally, through pet studies, it is recommended to incorporate L-carnitine in the hypocaloric diets of the obese dog because it modifies its body composition, allows to accentuate the loss of weight and stimulates the degradation of fat mass favoring the increase of muscle mass ( Gross and Zicker, 2000). Regards.

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Eliakunda
27 de julio de 2017

It is true that beyond the protein level we need more information on the levels of amino acids, however, the prevailing major limitation, especially to developing countries is not understanding the value of amino acids, but, availability and accessibility of analytical equipment and associated chemicals (if any) for amino acids.

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