RAPID DETECTION OF MYCOPLASMA BOVIS FROM MASTITIS MILK SAMPLES OF BUFFALO USING POLYMERASE CHAIN REACTION
Published: November 18, 2011
By: S. Nadeem*, S. U. Rahman*
Summary
This study describes the PCR based detection of Mycoplasma Bovis among other mycoplasmal contaminants causing buffalo mastitis. Mycoplasmal mastitis
caused by mycoplasma bovis is reported in dairy cattle but it is also present in variable quantity in buffalo. As an opportunistic pathogen causing mastitis, myoplasma bovis causes considerable economic losses to the dairy industry. Two hundred milking buffalo from Buffalo Research Institute Pattoki were screened for mycoplasma mastitis. Milk samples were collected from all the four quarters and for the detection of subclinical cases Whiteside Mastitis test was performed. Weekly collection showed increase in subclinical cases by 32 from 200 buffalo, 37 from 198 buffalo, 38 from 204 buffalo and 39 from 202 buffalo respectively. Subclinical cases and normal cases were then processed on modified freys medium for the detection of mycoplasma. Among other mycoplasma (M. bovigenitalium, M. bovirhinus, M. dispar) M. bovis was detected in 10 (33%) from 30 subclinical cases by traditional cultural method and biochemical testing. Thirty normal milk samples, positive broth samples and positive agar samples were screened by PCR technique which showed 10%, 60% and 73% prevalence
respectively as comparable to old traditional method 3.44%, 28% and 33% respectively. The results of PCR were available within 12 hr, which is far rapid than old
conventional culture method. Mycoplasma bovis was detected in more than 70% of mastitis milk samples by PCR method while by conventional culture method only 33% positive cases were detected.
Keywords: mastitis, mycoplasma, Polymerase Chain Reaction (PCR.)
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