sreenivas
Thanks for your question and sorry for late reply.
Chitosan is a biopolymer carrying positively charged ions. Therefore, when mixed with wastewater containing suspended particles which are largely negatively charged; charge neutralisation takes place. This reaction leads to aggregation of suspended particles into flocs. Heavy flocs then settle by gravity leaving a top supernatant clean water.
The above process is currently being carried out in my laboratory as a green technology for aquaculture wastewater treatment, moving away from chemical coagulants and flocculants.