The Development of An Animal-Specific Metagenomic Sequencing Pipeline to Increase Our Understanding of the Microbiome
Shaimaa Ahmed, Product Manager Diversigen
• The lack of complete animal-specific databases for microbiome studies has limited our understanding of the animal microbiome
• Diversigen has developed MetaGene™ Mouse, a mouse-specific shotgun metagenomic sequencing pipeline and a mouse-specific microbiome database that significantly improve the detection and identification of bacterial species and strains unique to mice, outperforming publically available databases
• MetaGene™ Mouse increases the overall median mapping rate from 12% to 45%. Furthermore, it increases the understanding of the microbiome function thanks to a 5-fold increase in functional mapping rate based on our mouse-specific database.
• The approach we took with the mouse microbiome can be applied to any animal species, including companion, and production, to facilitate a better understanding of each animal’s unique microbiome