Vaccination Against Infectious Bronchitis and Infectious Bursal Disease in poultry

The author misinterpreted the results in Table no.2 for 1 day of age class. Vaccine effects could not be demonstrated since unvaccinated controls had the same non-significant antibody titer as compared to vaccination ones. Else, you could make a mistake in running your ELISA since control titers of unvaccinated controls were always found and at high levels.
Multiple range tests among treatments were wrongly indicated for 1 day of age class in Table Nos 3 and 4. For instance, G3 was A (4929) , G1 was B (2943) then it impossible that G2 with titer of 5451 was AB ( Table 3).
Better rewrite the article and consult someone on statistical analysis and data interpretation.

Nice article but I have a comment on Ab titer in Table(3) regarding vaccination at age 7 days without to determine the optimal time of immunization may give you the result will be affected with maternal Ab and will give this difference, I hope for you all the best.

Regardless of all vaccination programs, whether this program is good or that program, first of all I hope we isolate the local strains and prepare vaccines from them then applying the best program, because antigenic variation can induce failures on the vaccination processes due to the difference of antigenic structures between vaccinal and field viruses, many out breaks of IB appeared in most provinces in Iraq, it was thought that they were due to vaccination with unsuitable strain of IB, all the IB vaccines, which used are commercially imported and are not matched with serotypes that exist in layers and broilers in Iraq and kurdistan region. Consequently, this may emerge new variant strains of IB viruses, also there are many publications revealed that IB vaccines have immunosuppressive effect on local immunity of Newcastle disease vaccines.

So it's better not to vaccination unless isolation of local strain of IB and prepare vaccines from them, because of unsuitabile vaccine lead to emerging new varient strains (rolling factor) and it's immunosuppressive effect on local immunity of Newcastle disease which is most important disease than IB in Iraq and kurdistan region.

Article presentation is good and timely. In table no 2, we got post vaccination titre for Treatment 1 as 1542, are we sure it is a protective titre. Though protective level differs from one elisa kit to other, i think it should b min of 5000.

From my field experience in Egypt and other Arab countries , always using a dead vaccine at day old of IB-ND-IBD
0.2 ml S/c at day old specially in endemic areas simultaneously with the recommended doses of life vaccines for the same mentioned diseases. This method of vaccination will be reflected on mortality rates, body gain & FCR

I am the student of M.Sc poultry science, my research topic is related to infectious bronchitis and vaccine preparation against IBV. I want you let me know how can I propagate the IB virus in laboratory for vaccine production? and what is the best method of killing this virus to prepare killed vaccines?

Haider Mansour
Doctor in vet hospital in Najaf /Iraq
These programs especially IB vaccine is not useful in my country because these strains don't match with the local strain.
To get a good vaccine for IB you must isolate the local strain and make from it vaccine this your method applied in my area and failure it.