Detection of Mycoplasma Hyopneumoniae Infections in Pigs of Nepal by Elisa

Mycoplasma hyopneumoniae infection is one of the causes of respiratory disease in pigs which is the primary agent in enzootic pneumonia. Enzootic Pneumonia is a chronic disease with low mortality but high morbidity causing economic loss in swine production through retarded growth, poor feed conversion, and predisposal to bacterial pulmonary infections. In Nepal, pig farming is increasing widely and the respiratory and reproductivity problem is also increasing causing loss to the farmers. Therefore this study aims to reveal the status of Mycoplasma infections in pigs.

A descriptive cross sectional study was carried out in pigs of Chitwan, Kathmandu and far-western region of Nepal from January, 2014 to January 2015 at Animal Health Research Division, Nepal. Blood samples of pigs from ear vein were collected and serum was separated and stored in deep freeze at -800C until tested. Samples were tested by ELISA for the detection of the antibodies against the M hyopneumonia.

Out of 384 pig sera, 59 sera were found positive, 13 sera were found doubtful indicating 15.33% prevalence. Pigs rearing under confinement (modern and semi conventional) were found significant in causing disease. Prevalence rate was higher in improved breeds compared to Local breeds. Age wise prevalence showed highest rate of infection during growing period.

This study therefore confirms the exposure to Mycoplasma infections in pigs of Nepal. Environmental and management factors contribute a major role in introduction and spread of this disease. Further study needs to be conducted on the strains of M hyopneumonia, epidemiology and control of infection.

Disclosure of Interest: None Declared.